EMAGE mouse gene expression spatial database

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EMAGE:11333

Slc6a13 solute carrier family 6 (neurotransmitter transporter, GABA), member 13 ( MGI:95629)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003550. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003550_01	euxassay_003550_02	euxassay_003550_03	euxassay_003550_04

euxassay_003550_05	euxassay_003550_06	euxassay_003550_07	euxassay_003550_08	euxassay_003550_09

euxassay_003550_10	euxassay_003550_11	euxassay_003550_12	euxassay_003550_13	euxassay_003550_14

euxassay_003550_15	euxassay_003550_16	euxassay_003550_17	euxassay_003550_18	euxassay_003550_19

euxassay_003550_20	euxassay_003550_21	euxassay_003550_22	euxassay_003550_23	euxassay_003550_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11333_wholemount_strong.wlz

11333_wholemount_moderate.wlz

11333_wholemount_weak.wlz

11333_wholemount_possible.wlz

11333_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11333_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon meninges

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

telencephalon meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain meninges

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain meninges

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

spinal cord meninges

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23 24

kidney calyx

moderate

regional

moderate expression: see section 08 09 10 11 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7410
Entity Detected:	Slc6a13, solute carrier family 6 (neurotransmitter transporter, GABA), member 13 ( MGI:95629)
Sequence:	sense strand is shown >T7410 CTCCGACAAGACCTCAGGCAGAAACTTCACCAAGGTGGGATGGAGAACAGGGCCTCGGGAACAACCAGTA ATGGAGAGACAAAGCCAGTGTGTCCAGCCATGGAGAAAGTGGAGGAAGATGGTACCCTGGAACGGGAGCA CTGGAACAACAAGATGGAGTTTGTGCTGTCTGTGGCTGGGGAGATCATTGGCTTAGGCAACGTCTGGAGG TTTCCCTATCTCTGCTACAAGAACGGGGGAGGTGCCTTCTTTATCCCCTACCTCATCTTCCTTTTTACCT GTGGCATCCCTGTCTTCTTCTTGGAGACAGCGCTTGGCCAGTACACCAACCAGGGAGGCATCACAGCCTG GAGGAGAATCTGTCCCATCTTTGAAGGCATCGGCTATGCCTCACAGATGATCGTCAGCCTCCTCAATGTC TACTACATCGTTGTCCTGGCCTGGGCCCTCTTCTACCTCTTCAGCAGCTTCACCACTGACCTCCCCTGGG GAAGTTGCAGCCATGAGTGGAATACAGAAAACTGTGTGGAATTCCAGAAGGCCAATGATTCCATGAATGT GACCTCCGAGAATGCCACCTCCCCTGTCATCGAGTTCTGGGAGAGGCGAGTCCTGAAGCTCTCGGATGGC ATCCAGCACCTGGGGTCCCTGCGCTGGGAACTGGTCCTGTGCCTCCTGCTCGCCTGGATCATCTGCTACT TCTGCATCTGGAAGGGGGTCAAGTCCACGGGCAAGGTGGTCTACTTCACAGCCACTTTCCCTTACCTCAT GCTGGTGGTCCTGTTGATTCGAGGAGTGACACTGCCCGGAGCAGCCCAAGGAATTCAGTTTTACCTGTAT CCCAACATCACACGTCTGTGGGATCCCCAGGTGTGGATGGATGCAGGCACCCAGATCTTCTTCTCCTTTG CCATCTGTCTGGGGTGCCTCACGGCCCTGGGAAGCTACAACAAGTACCACAACAATTGCTACAGGGACTG CATCGCCCTTTGCATCCTCAACAGCAGCACCAGCTTCATGGCCGGGTTTGCTATCTTCTCCATCCTGGGC TTCATGTCTCAGGAGCAGGGTGTGCCCATATCTGAGGTTGCTGAATCAGGCCCTGGCCTGGCATTCATCG CCTACCCTCGAGCTGTGGTGATGTTACCCTTCTCACCGTTGTGGGCCTGCTGTTTCTTCTTCATGGTCGT TCTCCTGGGACTAGATAGCCAGTTTGTGTGTGTAGAAAGCCTTGTGACAGCATTAGTGGACATGTATCCC CGGGTGTTCCGTAAGAAGAACCGGAGGGAGGTCCTCATCCTTATTGTGTCCGTCATCTCGTTCTTCATCG GGCTCATCATGCTCACAGAGGGCGGCATGTACGTGTTCCAGCTCTTTGACTACTATGCAGCCAGTGGCAT GTGTCTTCTCTTCGTGGCCATCTTTGAGTCCCTCTGTGTGGCTTGGGTTTATGGAGCCGGCCGCTTCTAC GACAACATTGAGGATATGATTGGGTATAAGCCATGGCCTCTTATCAAATATTGTTGGCTCTTTTTCACGC CAGCTGTGTGCCTGGCGACCTTCCTGTTCTCCCTGATCAAATACACGCCACTGACCTACAACAAGAAGTA CACGTACCCATGGTGGGGGGATGCCCTAGGGTGGCTCCTAGCTCTGTCCTCCATGATCTGCATTCCTGCC TGGAGCATCTACAAGCTGAGGACTCTCAAGGGCCCACTCAGAGAGAGACTTCGCCAGCTCGTGTGCCCGG CTGAAGACCTTCCCCAGAAGAACCAACCAGAGCCAACTGCTCCTGCGACACCGATGACATCCCTTCTCAG GCTCACAGAACTGGAGTCTAACTGCTAGGGCCAGGGCCTTTGACACACCTGTGAGCCTGGCTGTGGGGGC AGCTATAGAGGCAGAGGGGCGGAACTACCCCTCTGTGGTGGGGCAGAAAGACGAAAGGGGGTATTTGGGA CTCACCTTCTGAACTGGGTACCTCCTATCTTGATGCCCCTATTCCAGGGTGGTCCACACATTGTGACGTG CCCGAATCAATAACATCCCATTGAGGCAGCTTGGGAAGCCCAGGGAGGGCTGGGGAGGCAAAAGCCTGGC TTGTTGCTGGGCCCTCTTCCTTCCATGTCATTAAATCCAACTTCCTCTCAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4195373 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4195373 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11330 same embryo
	EMAGE:11334 same embryo
	EMAGE:11332 same embryo
	EMAGE:11329 same embryo
	EMAGE:11331 same embryo
	EurExpress:euxassay_003550 same experiment
	MGI:4828260 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source