EMAGE mouse gene expression spatial database

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EMAGE:11343

Cdc42ep4 CDC42 effector protein (Rho GTPase binding) 4 ( MGI:1929760)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003531. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003531_01	euxassay_003531_02	euxassay_003531_03	euxassay_003531_04

euxassay_003531_05	euxassay_003531_06	euxassay_003531_07	euxassay_003531_08	euxassay_003531_09

euxassay_003531_10	euxassay_003531_11	euxassay_003531_12	euxassay_003531_13	euxassay_003531_14

euxassay_003531_15	euxassay_003531_16	euxassay_003531_17	euxassay_003531_18	euxassay_003531_19

euxassay_003531_20	euxassay_003531_21	euxassay_003531_22	euxassay_003531_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11343_wholemount_strong.wlz

11343_wholemount_moderate.wlz

11343_wholemount_weak.wlz

11343_wholemount_possible.wlz

11343_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11343_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 03 04 05 06 17 18 19

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 07 08 09 13

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 weak expression: see section 04 05

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 14 weak expression: see section 10 11 12 13

pons ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 14 15 16 17

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

spinal cord ventricular layer

moderate

regional

moderate expression: see section 13 14

left lung

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12

right lung

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5957
Entity Detected:	Cdc42ep4, CDC42 effector protein (Rho GTPase binding) 4 ( MGI:1929760)
Sequence:	sense strand is shown >T5957 GCCATGGGTCGCCAGCCTTGCCGAGGCGCCAAGCACACCGAGGGGAGCGTACAGCCGCACCTGGTCCGCG CTCGGGGAGCTGGGGACCGAGCCCTCTGATTGCCCTGTCTACCCTTTGCATTGCTGGACTTCAGATCTGA CCCCATACCTGCCTGTTGCCTTGGGAGTGCCCAGCTCCCCCGCAGCCAGCACGATGCCCATTCTCAAACA GCTGGTGTCCAGCTCTGTGAACTCGAAGCGCCGCTCACGTGCGGACCTCACAGCCGAGATGATCAGTGCC CCGCTGGGTGACTTCCGCCACACCATGCATGTGGGCCGGGCTGGGGACGCCTTTGGGGACACGTCTTTCC TCACTAGCAAGGCCAGGGAGGCAGACGACGAGTCCCTGGATGAACAGGCCTCGGCTTCCAAGCTCAGCCT CCTGTCCAGGAAGTTCCGGGGCAGCAAGCGTTCACAGTCCGTGACCAGAGGGGACCGGGAGCAGAGAGAC ATGCTGGGCTCCCTGCGGGACTCAGCACTGTTTGTCAAGAATGCCATGTCCCTGCCTCAGCTCAATGAGA AGGAAGCCGCGGAGAAGGACTCGAGCAAGCTGCCCAAGAGCCTGTCGTCCAGCCCTGTGAAGAAGGCAGA CGCTAGAGATGGTGGCCCGAAGAGTCCCCATCGGAACGGGGCCACAGGCCCCCACTCACCTGACCCACTC CTTGACGAGCAGGCCTTTGGGGACCTGATGGATCTGCCCATCATGCCCAAAGTCAGCTACGGGCTGAAGC ATGCAGAGTCGATCCTGTCCTTCCACATCGACCTGGGGCCTTCCATGCTGGGAGATGTTCTCAGCATCAT GGACAAGGACCAGTGGGGCTCAGAGGAGGAGGAGGAAGCTGGCGGGTACCGTGACAAGGAAGGCCCCAGC AGCATTGTCCAGGCGCCCCCTGTGCTGGAGGTGGTTCCTCCTCTAGGGAGACAGGAAAGCAAGGCCAGCT GGGACCAGGCCTCTATGCTGCCCCCCCACGCTGTGGAGGATGACGGATGGGCGGTGGTAGCCCCCAGCCC CAGCTCAGCACGCAGTGTGGGCAGCCACACCACTCGGGACAGCAGCTCCCTGTCCAGCTACACCTCAGGC GTCCTTGAGGAGCGCAGCCCAGCTTTCAGAGGCCCAGACAGGGTGGCGGCTGCTCCCCCAAGGCAGCCAG ACAAGGAATTCTGCTTCATGGATGAGGAGGAGGAAGATGAGATCCGAGTTTGAGGCTGGACCGAAAGTTG AAAGTCGCCTCCACATCTCTGGGCTGCATCTTTTCCCTGCTGCTGCTGCCGCTGCCACCTTCTCTATGAC CTTTGACCTTACTCTGTAGGTGCAGCTAAGAGCCTCGTTGGAGTAGACCCTGGACCTCAGGGATTGGGGT GCCAGGGAAGTAAAGTAGCAGGGGCACTGGTGCCTGGTGGCCTGACTCTGACCTAACCTTCCTTACCACA CCAGCCTGGGACGGATCCGAGCCCCAGCGGGAAGCCATTCTCTTCCAGGCCCTTTGCTCCCAGCCTGGCC TCAGATGGATGCCAGATGTTAGTTTGAGTTGCTGCCGCAATGGCAGGGAAGCCAGGTGTGGGCTTCTCTC CAAGGTCATGGACACTTGGCTCCCTTCTGTTCACCTTGTGTCTGGAGCCTACCCAGGAGATATATGCAGC AGGAGGCAGGGGTGCTCACGTGTTTGAGCTCCTCCTTCTTCCACCTCTGGCTCCTCACTGAAGCCTGGTA TTCTTTGGGGTTCAGCTGAAGCCAGGGGACGGCCTCAACTATTACACTAAATGCAGCCTGTCCCAGAGGC TGCATCCCAGGACAGGATGGGGCACAGAAGGAACGAACACCCCCTGCTTCTAGCTTCTTGGGCTCTCAAG ACACTAGGAACAGGAAAGGGGCCAGCTCTGCCCATGGGTGTGTTTGTTCCTTGTTACTATGTTTTAAACC TGAATAGAGCACTGCCAAAATCCCCTGCCAGGAGACTAGTGACCGTCTTGGTGGCTGAGTGACTGTTATC TCACCTGAATCTGGTGTACAGACCCTGCCCAGGTTGGACTGTGGCGGGGCAGCCACCACCAGTGTTTGTC CCGGAACCCCCACCTCCATAGCTGTTAGTGGCTGAAGGAGGGCTGTGTAGGAAAAGTTCTGGAATACGAT GCTTAAATGATACATTATTCCCCGGGGTGTGAGCTACGCTTTGGCTAGTTAGTGGCCTCTCCTTGGGCTC TGGGCCAGGCCTCGCAAAAACACAAAACAAGACGGGACTGTATTTGATTGGAATTCCAGGGAAATGAAAG TGGGTTTTTTGTTTGTTTGTTTTTGGCTTTTTGGGATACGGTCTCACTCTAGCCCAAGCCGGCCTCAAGC TCATAGCGAGCCTCCCTTACCCTAGGAGCTTCGGTTGCAAGTGTGCACCACTGCTCCTGGCTGTTTTGTT CTTCTGAAAGCTGTTTTGCTCAGCTCCTGTAACTCTACCCAGACCGCTAGTAGAGGCAGGGGCCTGAGCC CTGGGGGTGGTAGCTCATTACTTTCCTGTGCTGGGAGCAGGCTGACCTGCTCCCTGGTCTAGCACAGCCA GTGGGAATCTGGGGAGAGAGCAAGCTAGATTACAAGCAGAGTCTGGCCAGATGGTGGCCAGCCATGGGCT GCTGTGCTTCCAGAGGCCACCCTCCTAGATTCAGACCCTGAAGTGCTCACTGGGCCCCTGACAGGTGCTC CTACGCAGGTAGGCAGTGACTGAGCTACAGAGCTGTGCCTCTGGCCAGCAGTACACACACAACACACACA CACCCCTCTCCTAAATCTACCTGGTTGGTTCTAAACTCTAAACTTTATATTTTTTTCCATGACATTGAAA AAGCAGTAAGAAAACATTAAAATTTCCTCCTAACAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3484427 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3484427 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11347 same embryo
	EMAGE:11348 same embryo
	EMAGE:11346 same embryo
	EMAGE:11345 same embryo
	EMAGE:11344 same embryo
	EurExpress:euxassay_003531 same experiment
	MGI:4823754 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source