EMAGE mouse gene expression spatial database

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EMAGE:11346

Nelf nasal embryonic LHRH factor ( MGI:1861755)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003546. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003546_01	euxassay_003546_02	euxassay_003546_03	euxassay_003546_04

euxassay_003546_05	euxassay_003546_06	euxassay_003546_07	euxassay_003546_08	euxassay_003546_09

euxassay_003546_10	euxassay_003546_11	euxassay_003546_12	euxassay_003546_13	euxassay_003546_14

euxassay_003546_15	euxassay_003546_16	euxassay_003546_17	euxassay_003546_18	euxassay_003546_19

euxassay_003546_20	euxassay_003546_21	euxassay_003546_22	euxassay_003546_23	euxassay_003546_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11346_wholemount_strong.wlz

11346_wholemount_moderate.wlz

11346_wholemount_weak.wlz

11346_wholemount_possible.wlz

11346_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11346_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 13 14 15 16 weak expression: see section 06

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5943
Entity Detected:	Nelf, nasal embryonic LHRH factor ( MGI:1861755)
Sequence:	sense strand is shown >T5943 CGGACGCGTGGGCTGGACGCGCTGGGCGCTGGGTGCGGCCATGCGGGGCCTGGGCCTAAGTCTGACCCTG ACCCTGAGGCTGAGACCTAGCGCGACCTCAGGCGCCGTACGGCTCCCCCGGCAGAGGCGGCGGGCGCGAG GCGCGCTCTGAAATCCGGGGGATGCGCGGGCGCCGTGACCATGGGTGCCGCCGCCTCCAGGAGGAGGGCG CTGAGGAGCGAGGCCATGTCCTCGGTAGCGGCCAAAGTAAGAGCAGCCCGAGCGTTTGGAGAGTACCTGT CCCAGAGTCACCCTGAGAACCGCAACGGTGCAGACCACCTGCTGGCTGACGCCTACTCTGGCCACGACGG GTCCCCAGAGATGCAACCTGCACCCCAGAACAAGCGCCGCCTCTCCCTCGTCTCCAATGGCCGATATGAG GGCAGCATCTCAGATGAGGCAGTCAGCGGGAAGCCGGCTATAGAGGGCCCCCAGCCCCACGTGTACACCA TCTCTAGAGAGCCCGCCCTGCTACCTGGCTCTGAAGCTGAAGCCATTGAGCTAGCAGTGGTAAAAGGGAG GAGACAGCGGGAACGGCACCCTCACCACCACAGCCAGCCCCTGCGTGCCAGCCCAGGCAGCAGCCGGGAG GACATCAGCAGGCCCTGCCAAAGCTGGGCAGGAAGCCGCCAGGGCTCCAAAGAGTGCCCAGGATGTGCCC AGCTGGTCCCTGGTCCCTCTTCTCGGGCCTTTGGGCTGGAGCAGCCACCTCTACCTGAGGCTCCTGGCCG CCACAAGAAGCTGGAAAGGATGTATAGCGTTGATGGAGTGTCTGATGATGTCCCCATCCGTACCTGGTTC CCCAAGGAAAACCTTTTCAGCTTCCAGACGGCAACCACAACTATGCAAGCGGTATTCAGGGGCTACGCGG AGAGGAAGCGTCGGAAACGGGAGAATGATTCCGCGTCTGTAATCCAGAGGAACTTCCGCAAACACCTGCG CATGGTTGGCAGTCGGCGGGTGAAGGCCCAGACGTTCGCTGAGCGTCGCGAACGGAGCTTCAGCCGGTCC TGGAGCGACCCCACCCCTATGAAAGCCGACACTTCCCACGACTCCCGAGACAGCAGTGACCTACAGAGCT CACACTGCACCCTGGATGAGGCTTGTGAGGACCTGGACTGGGACACAGAGAAAGGTCTGGAGGCCATGGC CTGCAACACCGAGGGCTTCTTGCCACCCAAGGTCATGCTCATCTCCTCCAAGGTGCCAAAGGCCGAGTAT ATCCCTACTATCATCCGCAGAGATGACCCGTCCATCATTCCCATCCTCTACGACCACGAGCATGCAACCT TCGAGGACATCCTGGAGGAAATAGAGAAGAAATTGAACATCTATCACAAGGGGGCCAAGATCTGGAAGAT GCTGATTTTCTGCCAGGGCGGCCCTGGACACCTTTATTTGCTCAAGAACAAGGTGGCCACCTTTGCCAAA GTGGAGAAGGAAGAGGACATGATCCACTTCTGGAAGAGGCTGAGCCGCCTGATGAGCAAGGTGAACCCCG AGCCGAATGTCATCCACATCATGGGCTGCTACATTCTGGGGAACCCTAACGGGGAGAAGCTATTCCAGAA CCTCAGGACCCTCATGACCCCTTACAAGGTTACCTTTGAGTCGCCTCTCGAGCTGTCTGCCCAAGGGAAG CAGATGATTGAGACCTACTTTGACTTCCGGCTGTACCGCCTGTGGAAGAGCCGCCAGCACTCAAAGCTGC TGGACTTTGATGACGTCCTGTGAGAAGCATGGGTCATGCCCAGGAACCACCAGGCCATTTCCTCTGGGCT GGGGACCTAGAGCTGGGCTGCCTCTTGCACCCGGGACAGTGTGGCCCTGCTCTTGCCCACAGCCACTTCA GGGACCCTCAGACGTTGCTCAGGTTCTCGTAGGGTCTGGTCCTCACTGCACCAGTGGGCCACAGAGGCCA CACTCCTGGTGGTCCAGATTAACAATCTCAGCCCATGACCAGTGGCCTCTCAATGGCCAGGGCATTGACC TGTCATGCCTCGCCTGGAGGCAGCAGCATTGGGGATCCCCTCCATGGGGTTCATAGAAATAAGTGCAATT TTTTTACACCCCTGAAACAATTTGAAGGGAAGCAGCATTACTAATTAACTAGTTAAGCACTCTGCTACTA GTTACAGTGTAGACAACCAGCCCAGTGTGTGTTCAGGTCCTCCTGTCCCCCAGCCTCTCAGCCCTGCTGT ATTTGATCACCAGCACCAGGATGGCCCATAAGTGTGGGGCTAGCTCTGTCAGGCTGCCTAGCCTGGCCCA GTCTACACTGGCCAAATCTGGCCTGCTTTCACCCTTTTGTCACCCCTGGCTTACTTTGACCTCATCCTGG CAAGGGGTAGGCTGGGCAGTTAGGAGGGAAGAGCCTCACTCACCCTCCCTCTTGTCAGGGGAGCACTGGG TTGCCATCTGTTGCCCACCTCCTCAAGCCAACCCATTTCCCTTTGCTGCTGCTGAACTCGGCTTTTTCTA GCCTTGGTCTGGAAGCCACATCCCAGCACAGTGGCTTAGGGGTGTGGTGGGGACAGCCGGGGCTGGGCCT GGGAAGGCAAGAACCTCACCAGCATTGTGGTGGGGGGCCTAGATCTGTCACCCCAGGCTGGAGAGTCAAG TAGCTGGTGAACTGTGAGGTCCCCATGACTCCCTTCAGTCTTGTCCAGGGTCTAGCAGGGTGGGCTCTCT TGTACATAGTTGGAGCTTGGGGGCAGGCCCCCCTTTTGTAGAGCCTGGGGTCTGGGGACACCTGGCCGTG TCCCTGACCCAGGGGAGTTAGGATTGGGCACTGAACAATGTACGATATCCCTTAGAGTGACCATTAAACT TGACCCTCTGCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3483536 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3483536 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11347 same embryo
	EMAGE:11343 same embryo
	EMAGE:11348 same embryo
	EMAGE:11345 same embryo
	EMAGE:11344 same embryo
	EurExpress:euxassay_003546 same experiment
	MGI:4826664 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source