EMAGE mouse gene expression spatial database

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EMAGE:11375

Trem2 triggering receptor expressed on myeloid cells 2 ( MGI:1913150)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006762. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006762_01	euxassay_006762_02	euxassay_006762_03	euxassay_006762_04

euxassay_006762_05	euxassay_006762_06	euxassay_006762_07	euxassay_006762_08	euxassay_006762_09

euxassay_006762_10	euxassay_006762_11	euxassay_006762_12	euxassay_006762_13	euxassay_006762_14

euxassay_006762_15	euxassay_006762_16	euxassay_006762_17	euxassay_006762_18	euxassay_006762_19

euxassay_006762_20	euxassay_006762_21	euxassay_006762_22	euxassay_006762_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11375_wholemount_strong.wlz

11375_wholemount_moderate.wlz

11375_wholemount_weak.wlz

11375_wholemount_possible.wlz

11375_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11375_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

interdigital region between forelimb digits 1 and 2

moderate

single cell

moderate expression: see section 02 04 07

interdigital region between forelimb digits 2 and 3

moderate

single cell

moderate expression: see section 02 04 07

interdigital region between forelimb digits 3 and 4

moderate

single cell

moderate expression: see section 02 04 07

interdigital region between forelimb digits 4 and 5

moderate

single cell

moderate expression: see section 02 04 07

interdigital region between hindlimb digits 1 and 2

moderate

single cell

moderate expression: see section 08 09

interdigital region between hindlimb digits 2 and 3

moderate

single cell

moderate expression: see section 08 09

interdigital region between hindlimb digits 3 and 4

moderate

single cell

moderate expression: see section 08 09

interdigital region between hindlimb digits 4 and 5

moderate

single cell

moderate expression: see section 08 09

mesenchyme

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

body-wall mesenchyme

strong

single cell

strong expression: see section 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8170
Entity Detected:	Trem2, triggering receptor expressed on myeloid cells 2 ( MGI:1913150)
Sequence:	sense strand is shown >T8170 GGCACGAGGGCGCCTACCCTAGTCCTGACTGTTGCTCAATCCAGGAGCACAGTTCCTGTGGGCTGAGCCT GACTGGCTTGGTCATCTCTTTTCTGCACTTCAAGGGAAAAGCAAGATCTTGCACAAGGTCCCCTCCGGCT GGCTGCTGGCAAAGGAAAGGTGCCATGGGACCTCTCCACCAGTTTCTCCTGCTGCTGATCACAGCCCTGT CCCAAGCCCTCAACACCACGGTGCTGCAGGGCATGGCCGGCCAGTCCTTGAGGGTGTCATGTACTTATGA CGCCTTGAAGCACTGGGGGAGACGCAAGGCCTGGTGTCGGCAGCTGGGTGAGGAGGGCCCATGCCAGCGT GTGGTGAGCACACACGGTGTGTGGCTGCTGGCCTTCCTGAAGAAGCGGAATGGGAGCACAGTCATCGCAG ATGACACCCTTGCTGGAACCGTCACCATCACTCTGAAGAACCTCCAAGCCGGTGACGCGGGCCTCTACCA GTGTCAGAGTCTCCGAGGCCGAGAGGCTGAGGTCCTGCAGAAAGTACTGGTGGAGGTGCTGGAGGACCCT CTAGATGACCAAGATGCTGGAGATCTCTGGGTCCCCGAGGAGTCATCGAGTTTCGAGGGTGCCCAAGTGG AACACAGCACCTCCAGGAATCAAGAGACCTCCTTCCCACCCACCTCCATTCTTCTCCTCCTGGCCTGCGT TCTCCTGAGCAAGTTTCTTGCAGCCAGCATCCTCTGGGCTGTGGCCAGGGGCAGGCAGAAGCCGGGAACA CCTGTGGTCAGAGGGCTGGACTGTGGCCAAGATGCTGGGCACCAACTTCAGATCCTCACTGGACCCGGAG GTACGTGAGAGAATTCTGAGTGGGAGGAGAACTACAGCTTAAGTCCAGCCAGGAGTCAATCCAGCCTGCA TGCTCTCCCCTCCTCCACCAAGACTTCTGTTTCTGCTACTTTTGCTTCAGAGGCCGCCTCTGCCTCAAGC CCACCTATCCTGGGAGCAGGAATACTGGTGTGTACATCTGTGTTGAGTGGGGAAGACAGCTGGATGGTTG TCTGTCAACTTCTGCACTTTGGACATTAAACATTCTCCACACACCAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5057491 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5057491 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11377 same embryo
	EMAGE:11378 same embryo
	EMAGE:11376 same embryo
	EMAGE:11379 same embryo
	EurExpress:euxassay_006762 same experiment
	MGI:4828900 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source