EMAGE mouse gene expression spatial database

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EMAGE:11379

Rab27b RAB27b, member RAS oncogene family ( MGI:1931295)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006777. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006777_01	euxassay_006777_02	euxassay_006777_03	euxassay_006777_04

euxassay_006777_05	euxassay_006777_06	euxassay_006777_07	euxassay_006777_08	euxassay_006777_09

euxassay_006777_10	euxassay_006777_11	euxassay_006777_12	euxassay_006777_13	euxassay_006777_14

euxassay_006777_15	euxassay_006777_16	euxassay_006777_17	euxassay_006777_18	euxassay_006777_19

euxassay_006777_20	euxassay_006777_21	euxassay_006777_22	euxassay_006777_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11379_wholemount_strong.wlz

11379_wholemount_moderate.wlz

11379_wholemount_weak.wlz

11379_wholemount_possible.wlz

11379_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11379_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand

moderate

regional

moderate expression: see section 01 02 03 04 05 06

foot

moderate

regional

moderate expression: see section 04 05 06 07 08 21 22 23

leg

moderate

regional

moderate expression: see section 03

mesenchyme

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

adenohypophysis

moderate

regional

moderate expression: see section 10 11 12 13 weak expression: see section 14

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

telencephalon mantle layer

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 06 07 13 14 weak expression: see section 05 08 09 10 11 12 15 16 17

pons mantle layer

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain mantle layer

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 16 moderate expression: see section 05 06 17

trigeminal v ganglion

strong

regional

strong expression: see section 16 17 20 21 moderate expression: see section 03 04 05 06 07 08 09 18 19

spinal cord mantle layer

weak

regional

weak expression: see section 08 09 10 11 12 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 14 15 16 weak expression: see section 10 11 12

pharyngo-tympanic tube

strong

regional

strong expression: see section 03 04 05 06 07 08 09 15 16 17 18 19 20 moderate expression: see section 01 02 weak expression: see section 21 22 23

conjunctival sac

strong

regional

strong expression: see section 04 22 23 moderate expression: see section 01 02 03 05

anterior naris

strong

regional

strong expression: see section 12 13 14 15 16 17

external naris

strong

regional

strong expression: see section 12 17

posterior naris

strong

regional

strong expression: see section 12

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 15 16 17 moderate expression: see section 09 18 19

naso-lacrimal duct

moderate

regional

moderate expression: see section 04 05 06 07 08 09 18 19 20 21 22 23

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10

midgut

moderate

regional

moderate expression: see section 11 12 13 14 15 16 weak expression: see section 17 18

mandible

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 16 17 18 19 20 21 22

maxilla

moderate

regional

moderate expression: see section 06 07 08 09 17 18 19 20 21 22

liver

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

bladder

strong

regional

strong expression: see section 13 14 15

urethra of male

strong

regional

strong expression: see section 13 14

left lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12

right lung

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8174
Entity Detected:	Rab27b, RAB27b, member RAS oncogene family ( MGI:1931295)
Sequence:	sense strand is shown >T8174 CGATCAGCAGCCACAGGTTGCAGACGCAAGGAGCAGTGCCCATGACCGCGACTCTGTGAGGCTGGCTGCC GGCGCGCGGTGGTTGCTGGCGGGTACCCGGAGCTCGGGAAGACCGGAGCGGGCCCCGAAACCTGACACCA GAGCCCTCGCTGCCCCCGCTCCGCGGGCTGGCCCTCCAAGACCATCACTATGACTGATGGAGACTATGAT TATCTGATTAAACTCCTGGCCCTTGGAGACTCAGGAGTCGGGAAGACAACATTTCTCTATAGATACACAG ACAATAAATTCAATCCCAAATTCATCACCACAGTGGGAATAGATTTTCGGGAAAAACGTGTGGTTTATGA CACACAAGGAGCAGATGGAGCGTCAGGAAAAGCGTTTAAGGTACATCTGCAGCTTTGGGACACTGCTGGA CAAGAGCGGTTCCGGAGCCTTACCACTGCCTTCTTCAGAGATGCCATGGGCTTCTTACTGATGTTTGACC TCACCAGTCAACAGAGCTTCTTGAATGTCAGAAACTGGATGAGTCAACTGCAGGCAAATGCTTACTGTGA AAACCCAGACATAGTATTAATTGGCAACAAGGCAGACCTGCCAGACCAAAGGGAAGTCAATGAACGGCAA GCACGGGAGCTGGCTGAAAAATATGGCATACCATACTTCGAAACAAGCGCAGCTACAGGACAGAATGTAG AGAAGTCAGTGGAAACGCTTCTGGACTTAATCATGAAGAGAATGGAAAAGTGTGTAGAGAAGACACAGGT TCCGGACACTGTCAATGGAGGAAATTCTGGAAAGCTGGATGGGGAAAAGCCAGCAGAAAAGAAATGTGCC TGCTAGACACTCAGAGGAACCAGTGATGGAGAGCCCACCCAAGTCCGACGTCCTGAGACAATGTCAAACC ATGATGTTGTCATTGAGCAGTAGCTCATGAGCAATGGCATGTCTTTCTCCCCTGACCCCAATCTATTTTA ATAAAGCAGAGTAGTCATAGTGTTGAAAGAACTGTACTGTTATCCCGAGTCCCTTCCAAACAAAAAAGGC AAAGTTGTCCTAAGGTGATCTCACTGTTGTGAATGCCCAGTTGTATTTTTATAAGGTTGTATATATGGGG AAAAAAAGTATCGGTGAGTTTTAAATGAAAGCACATATTACTGCCAGCGTGGAGAGAGGAAGAGTCTAAT TAAAGGGAGGATATAAAAGGATGATTTCTGTTTTCATCTACTTATGTGGAGTATGACTCACACTTCTATA TTTGCACTGGGGAAGACATATTCCTAAAAATGGATACATAACAAAGAATTCTCTGTGACTTATTAAGAAT GACTGTGTCTGCCCAGTAACCTGTTCTGAGTTACTGGTAAAGTCGTGGGTGGGTGAATGGCAGGCTTCTG TAGGAACTTGTGTAATTATGCCATTTTTATATTCCTAATAAATGGCAAAGAGACAAGAATAGGACCTTGG ATGGAAATTCCCTGGCAGAAAATGAAACATGGGAATAAGATGCTTGAACAAGTTAGATGAAATGGATCTT TGAGATAAGTCTGAGAGACAGAGTTACTTAGACAAATGACACTGAAGCAGATTAGAAGCTAGTATCACCC ATTGGGATTGGCAATGCCTTCAAAGGCTCCTAGTCAAATGATCCAAACCTTTAACATCGTTTCTGATGGT ACCCAAGTTCACATTTTATTTTTATTTTTTATCAAATTCTTAGAAAGAGTCATAAGAAAAAAAAAAGACA TTTTATTGTAAATAACTTACAAGTGAAATTAAGAAAACATAAGATTTAGTCTTACAGTTTCTAAATATAT GATTTGTTGTTGAGAGAAAACAGTGATTGTTAATTATGGCTTTTACTTCATTTTAGAAAAGCACTAGAAA TTCTAAATTTACTTTTCCTCTAGACCACACTTAATGCCTGCCTTGAAGACATTACAGAAATGAAGGTGTG TTCATGGGTCAATAACAGCAAAGTAATTATGGACCAATAACTATGTTCACCCTGAAACTTTTATGCCACC AATCACCAAAAATTGATACCACATTCTTTATTATAATGCTTTATACTTTGCCAGGAAGAAGTATAGCTAT AGTTGAGGTGTACCTAAGAAATTAGCAAGAATGGATCTGCGTCTCAGCTCAGCAGAACTATAGCCAATGT TTTGTTTTGTTTTCCTGAATGCTATCTTATTTTTTTTCTTATAGAATTAATCACAGGTCTTCTTATCATT GTGAAAACTCATGTTGTATTACTTTTTATTAATTGTAATTTGGGATACATATAAGTTAATCTTATCAAAT AATATTTAAAATTGTCAATCAATTTTACCAATAACAAAGCCAATAATATACTGTTAACAAAGAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5120275 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5120275 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11377 same embryo
	EMAGE:11378 same embryo
	EMAGE:11376 same embryo
	EMAGE:11375 same embryo
	EurExpress:euxassay_006777 same experiment
	MGI:4827571 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source