EMAGE mouse gene expression spatial database

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EMAGE:11411

Sdc1 syndecan 1 ( MGI:1349162)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003549_01	euxassay_003549_02	euxassay_003549_03	euxassay_003549_04	euxassay_003549_05

euxassay_003549_06	euxassay_003549_07	euxassay_003549_08	euxassay_003549_09	euxassay_003549_10

euxassay_003549_11	euxassay_003549_12	euxassay_003549_13	euxassay_003549_14	euxassay_003549_15

euxassay_003549_16	euxassay_003549_17	euxassay_003549_18	euxassay_003549_19	euxassay_003549_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11411_wholemount_strong.wlz

11411_wholemount_moderate.wlz

11411_wholemount_weak.wlz

11411_wholemount_possible.wlz

11411_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11411_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

strong

regional

strong expression: see section 05 06 07 08 09 10 11 18 19 20

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08

lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6262
Entity Detected:	Sdc1, syndecan 1 ( MGI:1349162)
Sequence:	sense strand is shown >T6262 CGGACGCGTGGGCGGACGCGTGGGCTCGGCGGGAGAGGTGCGGGCCAGAGGAGACAGAGCCTAACGCAGA GGAAGGGACCTGGCAGTCGGGAGCTGACTCCAGCCGGCGAAACCTACAGCCCTCGCTCGAGAGAGCAGCG AGCTGGGCAGGAGCCTGGGACAGCAAAGCGCAGAGCAATCAGCAGAGCCGGCCCGGAGCTCCGTGCAACC GGCAACTCGGATCCACGAAGCCCACCGAGCTCCCGCCGCCGGTCTGGGCAGCATGAGACGCGCGGCGCTC TGGCTTTGGCTCTGCGCGCTGGCGCTGCGCCTGCAGCCTGCCCTCCCGCAAATTGTGGCTGTAAATGTTC CTCCTGAAGATCAGGATGGCTCTGGGGATGACTCTGACAACTTCTCTGGCTCTGGCACAGGTGCTTTGCC AGATACTTTGTCACGGCAGACACCTTCCACTTGGAAGGACGTGTGGCTGTTGACAGCCACGCCCACAGCT CCAGAGCCCACCAGCAGCAACACCGAGACTGCTTTTACCTCTGTCCTGCCAGCCGGAGAGAAGCCCGAGG AGGGAGAGCCTGTGCTCCATGTAGAAGCAGAGCCTGGCTTCACTGCTCGGGACAAGGAAAAGGAGGTCAC CACCAGGCCCAGGGAGACCGTGCAGCTCCCCATCACCCAACGGGCCTCAACAGTCAGAGTCACCACAGCC CAGGCAGCTGTCACATCTCATCCGCACGGGGGCATGCAACCTGGCCTCCATGAGACCTCGGCTCCCACAG CACCTGGTCAACCTGACCATCAGCCTCCACGTGTGGAGGGTGGCGGCACTTCTGTCATCAAAGAGGTTGT CGAGGATGGAACTGCCAATCAGCTTCCCGCAGGAGAGGGCTCTGGAGAACAAGACTTCACCTTTGAAACA TCTGGGGAGAACACAGCTGTGGCTGCCGTAGAGCCCGGCCTGCGGAATCAGCCCCCGGTGGACGAAGGAG CCACAGGTGCTTCTCAGAGCCTTTTGGACAGGAAGGAAGTGCTGGGAGGTGTCATTGCCGGAGGCCTGGT GGGCCTCATCTTTGCTGTGTGCCTGGTGGCTTTCATGCTGTACCGGATGAAGAAGAAGGACGAAGGAAGC TACTCCTTGGAGGAGCCCAAACAAGCCAATGGCGGTGCCTACCAGAAACCCACCAAGCAGGAGGAGTTCT ACGCCTGATGGGGAAATAGTTCTTTCTCCCCCCACAGCCCCTGCCACTCACTAGGCTCCCACTTGCCTCT TCTGTGAAAAACTTCAAGCCCTGGCCTCCCCACCACTGGGTCATGTCCTCTGCACCCAGGCCCTTCCAGC TGTTCCTGCCTGAGTGGTCCCAGGGTGTGCTGGGAACTGATTCCCCTCCTTTGACTTCTGCCTAGAAGCT TGGGTGCAAAGGGTTTCTTGCATCTGATCTTTCTACCACAACCACACCTGTCGTCCACTCTTCTGACTTG GTTTCTCCAAATGGGAGGAGACCCAGCTCTGGACAGAAAGGGGACCCGACTGCTTTGGACCTAGATGGCC TATTGCGGCTGGAGGATCCTGAGGACAGGAGAGGGGCTTCGGCTGACCAGCCATAGCACTTACCCATAGA GACCGCTAGGGTTGGCCGTGCTGTGGTGGGGGATGGAGGCCTGGGCTCCTTGGAATCCACTTTTCATTGT GGGGAGGTCTACTTTAGACAACTTGGTTTTGCACATATTTTCTCTAATTTCTCTGTTCAGAGCCCCAGCA GACCTTATTACTGGGGTAAGGCAAGTCTGTTGACTGGTGTCCCTCACCTCGCTTCCCTAATCTACATTCA GGAGACCGAATCGGGGGTTAATAAGACTTTTTTTGTTTTTTGTTTTTGTTTTTAACCTAGAAGAACCAAA TCTGGACGCCAAAACGTAGGCTTAGTTTGTGTGTTGTCTCTGAGTTTGTCGCTCATGCGTACAACAGGGT ATGGACTATCTGTATGGTGCCCCATTTTTGGCGGCCCGTAAGTAGGCTGGCTAGTCCAGGATACTGTGGA ATAGCCACCTCTTGACCAGTCATGCCTGTGTGCATGGACTCAGGGCCACGGCCTTGGCCTGGGCCACCGT GACATTGGAAGAGCCTGTGTGAGAACTTACTCGAAGTTCACAGTCTAGGAGTGGAGGGGAGGAGACTGTA GAGTTTTGGGGGAGGGGTGGCAAGGGTGCCCAAGCATCTCCCACCTTTGGTACCATCTCTAGTCACCCTT TCTCCCGGAAGTTGACAAGACACATCTTGAGTATGGCTGGCACTGGTTCCTCCATCAAGAACCAAGTTCA CCTTCAGCTCCTGTGGCCCCGCCCCCAGGCTGGAGTCAGAAATGTTTCCCAAAGAGTGAGTCTTTTGCTT TTGGCAAAACGCTACTTAATCCAATGGGTTCTGTACAGTAGATTTTGCAGATGTAATAAACTTTAATATA AAGGAGTCCTATGAACTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3501359 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3501359 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11412 same embryo
	EMAGE:11409 same embryo
	EMAGE:11410 same embryo
	EMAGE:11413 same embryo
	EMAGE:11408 same embryo
	EurExpress:euxassay_003549 same experiment
	MGI:4827918 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source