EMAGE mouse gene expression spatial database

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EMAGE:11443

Cdc45 cell division cycle 45 homolog (S. cerevisiae) ( MGI:1338073)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006791. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006791_01	euxassay_006791_02	euxassay_006791_03	euxassay_006791_04

euxassay_006791_05	euxassay_006791_06	euxassay_006791_07	euxassay_006791_08	euxassay_006791_09

euxassay_006791_10	euxassay_006791_11	euxassay_006791_12	euxassay_006791_13	euxassay_006791_14

euxassay_006791_15	euxassay_006791_16	euxassay_006791_17	euxassay_006791_18	euxassay_006791_19

euxassay_006791_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11443_wholemount_strong.wlz

11443_wholemount_moderate.wlz

11443_wholemount_weak.wlz

11443_wholemount_possible.wlz

11443_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11443_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 05 06 13 14

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 10 11

telencephalon ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

rest of cerebellum marginal layer

strong

regional

strong expression: see section 03 04 05 06 07 08 11 13 14 15 16 17 18 19

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 05 06 13 14

midbrain ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8229
Entity Detected:	Cdc45, cell division cycle 45 homolog (S. cerevisiae) ( MGI:1338073)
Sequence:	sense strand is shown >T8229 GAGTCTTGACGACCTCCGGGGCTGTCCGTGTCTCAGCGCCAGTTCTAGGTATCCGCCGCTGTGGCTATGT TCGTGACCGATTTCCGCAAGGAGTTCTACGAGACGGTCCACAACCAGAGGGTCCTTCTCTTTGTGGCCTC GGACGTGGATGCCTTGTGTGCTTGCAAGATCCTCCAGGCCCTGTTTCAGTGTGACCACGTCCAGTATACG CTGGTTCCGGTTTCTGGGTGGCAAGAACTTGAAACTGCATATCTTGAACATAAAGAGCAGTTCTCCTATT TCATCCTCATAAACTGTGGAGCCAACGTGGACCTGTTGGATATCCTTCAGCCTGATGAAGACAGTATATT TTTTGTGTGTGATACCCACAGGCCAGTGAACGTTGTGAATGTGTACAATGACACTCAGATCAAATTGCTT ATTAAACAAGAGGATGACCTTGAGGTTCCTGCCTACGACGACATCTTTAGAGATGAAGCAGAGGATGAAG ATCTTTCAGACAGTGACGGTGATGGGTCAGAGCCTTCAGAGAAGCGCACACGGTTAGAAGAGGAGATAGT GGAGCGAAACAGGAAGAGGAGGCAGCGCAGGGAGTGGGAGGCCCGAAGGAAAGACATCCTCTTTGACTAC GAGCAGTATGAATATTATGGGACATCGTCGGCCATGGTGATGTTTGACCTGGCGTGGATGATGTCCAAGG ACCTGAATGACATGCTGTGGTGGGCCATTGTTGGACTGACAGACCAGTGGGTGCATGACAAGATCACTCA AATGAAGTACGTGACTGATGTTGGCATCCTGCAGCGTCATGTGTCCCGTCATAACCACCGAAACGAGGCG GAGGAGAACATGCTCTCTGTAGACTGCACCCGGATCTCCTTCGAGTATGACCTCTGCCTGGTACTGTATC AGCACTGGTCCCTCCATGAAAGCCTGTACAACACCAGCTACACAGCAGCCAGGTTTAAGCTCTGGTCTGT GCATGGGCAGAAACGACTCCAGGAGTTCCTTGCAGACATGGGGCTTCCCCTGAAGCAAGTCAAGCAGAAA TTTCAGTCCATGGACGTTTCCTTGAAGGGGAATCTGCGAGAAATGATTGAAGAATCTGCAAATAAATTTG GGATGAAGGACATGCGTGTACAGACTTTCAGCATTCAGTTTGGGTTCAAGCATAAATTCCTGGCCAGTGA TGTCGTCTTTGCCACCATGTCTCTAATGGAGAGTCCTGAGAAGGATGGCTCAGGGACAGACCACTTCATC CAGGCTCTCGATAGCCTCTCCAGGAGTAACCTGGACAAGCTATACCTTGGTCTAGAGCTCGCCAAGAAGC ACCTCCAAGCCACACAACAGACCATCGCCAGCTGTCTCTGTACCAACCTCGTCACTTCCCAGGGCCCTTT TCTCTACTGCTCACTCATGGAGGGCACTCCAGATGTCACCCTGTTTTCCAAGCCAGCATCCTTGAGTCTG CTCAGCAGACATCTGCTCAAGTCCTTTGTGTACTCGACAAAGAATCGACGATGCAAGCTGCTGCCCCTGG TGATGGCTGCCCCGCTGAGCGTGGAACAGGGCACAGTGACCGTGGTGGGCATCCCCCCAGAGACTGATAG CTCGGATAGAAAGAACTTTTTTGGTCGGGCTTTTGAGAAGGCAGCAGAAAGCACCAGCTCTCGGACTCTA CACAATTACTTTGACCTCTCAGTAATTGAGCTGAAGGCTGAGGACCGGAGCAAGTTCCTGGACGCTCTTG TGTCACTGCTGTCCTGAGGAATTCAACTTCTCCAGAAGTGACCTCCTTTTCCTTATTTATATTTCCTGGC CACTATTCAGTTGTAAGATAACATTTGAAATGTGAGACTATATGGTTTTTAATAAAATGTTTGATGTTTT AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1262927 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1262927 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11445 same embryo
	EMAGE:11442 same embryo
	EMAGE:11444 same embryo
	EMAGE:11441 same embryo
	EMAGE:11440 same embryo
	EurExpress:euxassay_006791 same experiment
	MGI:4823755 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source