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EMAGE:11453

Cryba4 crystallin, beta A4 ( MGI:102716)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11453 EMAGE:11453 EMAGE:11453 EMAGE:11453 EMAGE:11453
"Pseudo-wholemount" of euxassay_006916. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006916_01 euxassay_006916_02 euxassay_006916_03 euxassay_006916_04
EMAGE:11453 EMAGE:11453 EMAGE:11453 EMAGE:11453 EMAGE:11453
euxassay_006916_05 euxassay_006916_06 euxassay_006916_07 euxassay_006916_08 euxassay_006916_09
EMAGE:11453 EMAGE:11453 EMAGE:11453 EMAGE:11453 EMAGE:11453
euxassay_006916_10 euxassay_006916_11 euxassay_006916_12 euxassay_006916_13 euxassay_006916_14
EMAGE:11453 EMAGE:11453 EMAGE:11453 EMAGE:11453 EMAGE:11453
euxassay_006916_15 euxassay_006916_16 euxassay_006916_17 euxassay_006916_18 euxassay_006916_19
EMAGE:11453 EMAGE:11453 EMAGE:11453 EMAGE:11453
euxassay_006916_20 euxassay_006916_21 euxassay_006916_22 euxassay_006916_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11453Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11453_wholemount_strong.wlz
11453_wholemount_moderate.wlz
11453_wholemount_weak.wlz
11453_wholemount_possible.wlz
11453_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11453_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9117
Entity Detected:Cryba4, crystallin, beta A4 ( MGI:102716)
Sequence:sense strand is shown

>T9117
GGCCCTTCTTGGACCGTGCCAACATGACCCTGCAGTGCACCAAGTCAGCTGGACACTGGAGGATGGTGGT
GTGGGATGAAGAAGGCTTCCAGGGCCGACGGCATGAATTCACAGCTGAGTGTCCCAGTGTCCTGGAACTT
GGTTTTGAGACGGTGCGATCTCTCAAAGTCCTGAGCGGAGCGTGGGTAGGCTTTGAGCACGCCGGCTTCC
AAGGACAGCAATATGTGCTGGAGAGGGGCGATTACCCGGGCTGGGATGCCTGGGGTGGCAACACAGCCTA
CCCTGCGGAGAGGCTCACCTCCTTCCGGCCTGTGGCCTGCGCTAACCACCGCGACTCAAGGCTGACCATC
TTCGAGCAGGAGAACTTCCTGGGCAGGAAAGGCGAGCTGAACGATGACTATCCCTCTCTGCAGGCCATGG
GCTGGGACGGCACTGAAGTGGGCTCCTTCCATGTTCAATCTGGGGCGTGGGTTTGTTCCCAGTTTCCTGG
CTACCGAGGTTTTCAGTACATCCTGGAGAGCGATCACCACTCAGGTGACTACAAGCACTTCAGAGAGTGG
GGCTCCCATGCTCACACCTTCCAGGTGCAGAGTGTGCGCAGAATCCAGCAGTGAGCACGCACTGGCGACC
TGGGGTGCCTGCGAAACGACTGGGTGACTGGCCGGAGGATGTGGCTGCTCTTGGTTCTGGCTACCCTTGT
GTCCTCTGGGAACCCCACATCCCTGTCAGCCAGCCCATGCCCCGCCAGCTCATGGAGCTCAAAGAAATAA
AATGAAAAACAAAAGACTCAGAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6824293 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6824293 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11454 same embryo
 EMAGE:11457 same embryo
 EMAGE:11456 same embryo
 EMAGE:11458 same embryo
 EMAGE:11455 same embryo
 EurExpress:euxassay_006916 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS