EMAGE mouse gene expression spatial database

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EMAGE:11494

Hdac8 histone deacetylase 8 ( MGI:1917565)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006901. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006901_01	euxassay_006901_02	euxassay_006901_03	euxassay_006901_04

euxassay_006901_05	euxassay_006901_06	euxassay_006901_07	euxassay_006901_08	euxassay_006901_09

euxassay_006901_10	euxassay_006901_11	euxassay_006901_12	euxassay_006901_13	euxassay_006901_14

euxassay_006901_15	euxassay_006901_16	euxassay_006901_17	euxassay_006901_18	euxassay_006901_19

euxassay_006901_20	euxassay_006901_21	euxassay_006901_22	euxassay_006901_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11494_wholemount_strong.wlz

11494_wholemount_moderate.wlz

11494_wholemount_weak.wlz

11494_wholemount_possible.wlz

11494_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11494_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 13 14 15

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 09 10 11 12 moderate expression: see section 13 14

pons ventricular layer

strong

regional

strong expression: see section 09 10 11 12 16 17 18 moderate expression: see section 13 14 15

midbrain ventricular layer

strong

regional

strong expression: see section 12 13 14 15 16 moderate expression: see section 10 11

spinal cord ventricular layer

strong

regional

strong expression: see section 12 13 moderate expression: see section 11

esophagus epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12

pharynx epithelium

moderate

regional

moderate expression: see section 12 13

stomach

moderate

regional

moderate expression: see section 01 02 03

stomach proventricular region

moderate

regional

moderate expression: see section 04 05 06 07 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9946
Entity Detected:	Hdac8, histone deacetylase 8 ( MGI:1917565)
Sequence:	sense strand is shown >T9946 GGAGTCTGAAAACTGTTGGCTCCAGACTTTGGCTCGCGGACGGTTGGAAGTGGGAGATGGAGATGCCAGA GGAACCCGCCAACAGTGGGCATTCACTACCCCCGGTTTATATTTACAGTCCCGAGTATGTCAGCATCTGC GACTCCCTTGTGAAGGTCCCCAAACGGGCCAGTATGGTCCACTCTCTGATCGAAGCCTATGCCCTGCATA AACAAATGAGGATAGTGAAGCCCAAAGTGGCCTCCATGGAGGAGATGGCCACCTTCCACACTGATGCCTA TCTGCAACATCTCCAGAAAGTCAGCCAAGAAGGTGATGAGGACCATCCAGACTCCATAGAATATGGACTA GGTTATGACTGCCCAGCCACAGAAGGGATATTTGACTATGCAGCAGCTATAGGAGGAGGTACAATCACAG CTGCCCAGTGCCTGATTGACGGGAAGTGTAAAGTAGCCATCAACTGGTCTGGAGGGTGGCATCATGCAAA GAAAGATGAAGCATCTGGTTTCTGTTATCTCAATGATGCTGTCCTGGGAATATTACGATTGCGACGGAAA TTTGACCGTATTCTCTACGTGGATTTGGATCTACACCATGGAGATGGTGTAGAAGATGCCTTCAGTTTTA CATCGAAGGTTATGACTGTGTCCCTGCACAAGTTCTCCCCAGGATTTTTCCCAGGAACAGGTGACATGTC TGATGTTGGCCTGGGGAAAGGACGGTACTACAGTGTCAATGTGCCCATTCAGGATGGCATCCAGGATGAG AAGTACTATCACATCTGTGAAAGTGTACTAAAAGAAGTGTACCAGGCCTTCAATCCGAAGGCAGTGGTTT TACAGCTGGGAGCAGATACTATTGCCGGAGATCCAATGTGTTCCTTTAATATGACACCAGTGGGGATCGG CAAGTGTCTGAAGTATGTCCTACAGTGGCAGCTGGCAACTCTGATTTTAGGAGGAGGAGGCTATAACCTT GCCAACACGGCTCGTTGCTGGACATACTTGACCGGGGTCATCCTAGGGAAAACGCTATCCTCTGAGATCC CAGATCATGAGTTTTTCACAGCATATGGTCCTGATTATGTGCTGGAAATCACGCCAAGCTGCCGGCCAGA CCGCAATGAGCCCCACCGAATCCAGCAAATCCTCAACTACATCAAAGGGAATCTGAAGCATGTGGTCTAG TTGACAAAAAGAGATCAGGTTCCCAGAGCTGGGCAGCAGTGCCTATAATGAAGACAGTGTGTTGATGCAA GCGGTTTGTGGAATTTGTGACTGCAGGGGAAATTTGGAAGAAATTACTTCCTGAAAATTTCCAAGGGGCA CCAAGTGGCAGCTGGCCTCCTGGGGTGAGGAGCCAGGCACCCCGGAGCCAGGCCTAAGGGGAAAAAAAAA AAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30251620 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30251620 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11492 same embryo
	EMAGE:11490 same embryo
	EMAGE:11491 same embryo
	EMAGE:11495 same embryo
	EMAGE:11493 same embryo
	EurExpress:euxassay_006901 same experiment
	MGI:4825343 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source