EMAGE mouse gene expression spatial database

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EMAGE:11495

Casp4 caspase 4, apoptosis-related cysteine peptidase ( MGI:107700)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006935. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006935_01	euxassay_006935_02	euxassay_006935_03	euxassay_006935_04

euxassay_006935_05	euxassay_006935_06	euxassay_006935_07	euxassay_006935_08	euxassay_006935_09

euxassay_006935_10	euxassay_006935_11	euxassay_006935_12	euxassay_006935_13	euxassay_006935_14

euxassay_006935_15	euxassay_006935_16	euxassay_006935_17	euxassay_006935_18	euxassay_006935_19

euxassay_006935_20	euxassay_006935_21	euxassay_006935_22	euxassay_006935_23	euxassay_006935_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11495_wholemount_strong.wlz

11495_wholemount_moderate.wlz

11495_wholemount_weak.wlz

11495_wholemount_possible.wlz

11495_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11495_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

weak

spotted

weak expression: see section 12

thymus primordium

weak

spotted

weak expression: see section 11 12 13 14 15 16

heart ventricle

weak

spotted

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20

left lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13

right lung

moderate

regional

moderate expression: see section 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9950
Entity Detected:	Casp4, caspase 4, apoptosis-related cysteine peptidase ( MGI:107700)
Sequence:	sense strand is shown >T9950 ACTCTGTCAAGCTGTCTTCACGGTGCGAAAGAACTGAGGCTTTTTCTCATGGCTGAAAACAAACACCCTG ACAAACCACTTAAGGTGTTGGAACAGCTGGGCAAAGAAGTCCTTACGGAGTACCTAGAAAAATTAGTACA AAGCAATGTACTGAAATTAAAGGAGGAAGATAAACAAAAATTTAACAATGCTGAACGCAGTGACAAGCGT TGGGTTTTTGTAGATGCCATGAAAAAGAAACACAGCAAAGTAGGTGAAATGCTTCTCCAGACATTCTTCA GTGTGGACCCAGGCAGCCACCATGGTGAAGCTAATCTGGAAATGGAGGAACCAGAAGAATCATTGAACAC TCTCAAGCTTTGTTCCCCTGAAGAGTTCACAAGGCTTTGCAGAGAAAAGACACAAGAAATTTACCCAATA AAGAAGGCCAATGGCCGTACACGAAAGGCTCTTATCATATGCAATACAGAGTTCAAACATCTCTCACTGA GGTATGGGGCTAACTTTGACATCATTGGTATGAAAGGCCTTCTTGAAGACTTAGGCTACGATGTGGTGGT GAAAGAGGAGCTTACAGCAGAGGGCATGGAGTCAGAGATGAAAGACTTTGCTGCACTCTCAGAACACCAG ACATCAGACAGCACATTCCTGGTGCTAATGTCTCATGGCACACTGCATGGCATTTGTGGAACAATGCACA GTGAAAAAACTCCAGATGTGCTACAGTATGATACCATCTATCAGATATTCAACAATTGCCACTGTCCAGG TCTACGAGACAAACCCAAAGTCATCATTGTGCAGGCCTGCAGAGGTGGGAACTCTGGAGAAATGTGGATC AGAGAGTCTTCAAAACCCCAGTTGTGCAGAGGTGTAGATCTACCTAGGAATATGGAAGCTGATGCTGTCA AGCTGAGCCACGTGGAGAAGGACTTCATTGCCTTCTACTCTACAACCCCACATCACTTGTCCTACCGAGA CAAAACAGGAGGCTCTTACTTCATCACTAGACTCATTTCCTGCTTCCGGAAACATGCTTGCTCTTGTCAT CTCTTTGATATATTCCTGAAGGTGCAACAATCATTTGAAAAGGCAAGTATTCATTCCCAGATGCCCACCA TTGATCGGGCAACCTTGACGAGATATTTCTACCTCTTTCCTGGCAACTGAGAACAAAGCAACAAGCAACT GAATCTCATTTCTTCAGCTTGAAGAAGTGATCTTGGCCAAGGATCACATTCTATTCCTGAAATTCCAGAA CTAGTGAAATTAAGGAAAGAATACTTATGAATTCAAGACCAGCCTAAGCAACACAGTGGGATTCTGTTCC ATAGACAAGCAAACAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30251263 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30251263 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11492 same embryo
	EMAGE:11494 same embryo
	EMAGE:11490 same embryo
	EMAGE:11491 same embryo
	EMAGE:11493 same embryo
	EurExpress:euxassay_006935 same experiment
	MGI:4823642 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source