EMAGE mouse gene expression spatial database

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EMAGE:11512

Snrpd1 small nuclear ribonucleoprotein D1 ( MGI:98344)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006889. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006889_01	euxassay_006889_02	euxassay_006889_03	euxassay_006889_04

euxassay_006889_05	euxassay_006889_06	euxassay_006889_07	euxassay_006889_08	euxassay_006889_09

euxassay_006889_10	euxassay_006889_11	euxassay_006889_12	euxassay_006889_13	euxassay_006889_14

euxassay_006889_15	euxassay_006889_16	euxassay_006889_17	euxassay_006889_18	euxassay_006889_19

euxassay_006889_20	euxassay_006889_21	euxassay_006889_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11512_wholemount_strong.wlz

11512_wholemount_moderate.wlz

11512_wholemount_weak.wlz

11512_wholemount_possible.wlz

11512_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11512_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7048
Entity Detected:	Snrpd1, small nuclear ribonucleoprotein D1 ( MGI:98344)
Sequence:	sense strand is shown >T7048 CGGACGCGTGGGCTTTTCCGGCCATTCATAACCCCAGTCCGTGGATCTCTGGAGCCTGAATTCTGTGAAC AGTCGGGCCAGAAGGGAGAACTGCGTCGCTAGGATGAAGCTCGTGAGATTTTTGATGAAATTGAGTCATG AAACTGTAACCATTGAATTGAAGAATGGGACACAAGTCCATGGAACAATCACAGGTGTAGATGTCAGCAT GAACACACACCTTAAAGCTGTGAAAATGACCCTGAAGAACAGAGAACCTGTACAGTTGGAAACATTGAGT ATTCGAGGCAATAACATTCGGTATTTTATTCTACCAGACAGCTTACCTCTAGATACACTACTTGTGGATG TTGAACCTAAGGTGAAGTCTAAGAAAAGAGAAGCTGTTGCAGGAAGAGGCCGAGGCCGGGGTAGAGGAAG AGGACGTGGTCGTGGCAGAGGAAGAGGGGGTCCTAGGCGATAATGTCTCTCAAGATTACTGTTTCAAATT GATAAGGAATTTGGGGATATCTTTTGTACAGGTTTTGTTTGTCAGTTTTTAATAAACATAAGTGTAGGGA CAGAGTTGTCTGTTGATTGTCAAATTAAAGTTGTAGTGAGTATCTACTATATTCACATAGTGTGCTCAGT TCGGGGAGTTGGACGCATGAAATACATATGTACTGAACATGGGTTTGATTTCTTTGAGTCCATTATTTGG GCCTCTTTTGAGATGAAATAAATTCTTGGCATTTCAATAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4009014 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4009014 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11514 same embryo
	EMAGE:11513 same embryo
	EMAGE:11516 same embryo
	EMAGE:11517 same embryo
	EMAGE:11515 same embryo
	EurExpress:euxassay_006889 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source