EMAGE mouse gene expression spatial database

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EMAGE:11515

Pomgnt1 protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase ( MGI:1915523)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006877. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006877_01	euxassay_006877_02	euxassay_006877_03	euxassay_006877_04

euxassay_006877_05	euxassay_006877_06	euxassay_006877_07	euxassay_006877_08	euxassay_006877_09

euxassay_006877_10	euxassay_006877_11	euxassay_006877_12	euxassay_006877_13	euxassay_006877_14

euxassay_006877_15	euxassay_006877_16	euxassay_006877_17	euxassay_006877_18	euxassay_006877_19

euxassay_006877_20	euxassay_006877_21	euxassay_006877_22	euxassay_006877_23	euxassay_006877_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11515_wholemount_strong.wlz

11515_wholemount_moderate.wlz

11515_wholemount_weak.wlz

11515_wholemount_possible.wlz

11515_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11515_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

strong

regional

strong expression: see section 05 15 16 17 18 moderate expression: see section 06 07 08 09

4th ventricle

strong

regional

strong expression: see section 02 03 04 05 12 13 14 15 16 17 18 19 20 moderate expression: see section 06 07 08 09 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7331
Entity Detected:	Pomgnt1, protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase ( MGI:1915523)
Sequence:	sense strand is shown >T7331 GCGGGGCCCGGGGCCCCTGAGCCTCCCCTCAGATTCCTGAAGTCATGGACTGGCCGGGACAGGGGTGACT CCCTAATCCAGTATGGATGACTGGAAGCCCAGTCCCCTCATCAAGCCCTTTGGGGCTAGGAAGAAACGGA GCTGGTACCTTACCTGGAAGTATAAACTGACAAACCAGCGGGCCCTGAGGAGATTCTGTCAGACAGGGGC TGTGCTTTTCCTGCTGGTGACCGTCATTGTCAACATCAAGTTGATCCTGGACACTCGGAGAGCAATCAGC GAGGCCAATGAAGACCCAGAACCAGAGCAAGACTATGATGAGGCCCTAGGACGTCTGGAGTCCCCTCGGC GCAGAGGCAGCAGTCCCCGACGGGTGCTGGATGTGGAAGTATACTCCAGTAGAAGCAAAGTGTATGTGGC TGTGGATGGCACCACGGTGCTCGAGGATGAAGCCCGGGAGCAGGGCCGAGGCATCCATGTCATAGTCCTC AACCAGGCCACGGGCCACGTGATGGCAAAGCGCGTGTTTGACACATACTCTCCTCATGAGGATGAGGCCA TGGTGCTGTTCCTCAACATGGTGGCCCCCGGCCGCGTGCTGATCTGCACGGTGAAGGATGAGGGTTCCTT CCACCTCAAGGACACAGCAAAGGCTCTGCTGAGGAGCCTGGGAAGTCAGGCAGGCCCTGCCCTTGGCTGG AGGGACACCTGGGCCTTCGTGGGACGAAAAGGAGGTCCTGTACTTGGCGAGAAACATTCTAAATCTCCTG CCCTCTCCTCCTGGGGTGACCCAGTCCTGCTGAAGACAGATGTGCCACTGAGCTCAGCTGAAGAGGCTGA GTGTCACTGGGCAGATACGGAGTTGAACCGCCGCCGCAGGCGCTTCTGCAGCAAAGTGGAGGGCTACGGG AGCGTGTGCAGCTGCAAGGACCCGACGCCTATTGAGTTCAGCCCTGACCCACTTCCAGACAATAAGGTCC TCAATGTGCCTGTGGCTGTCATTGCAGGGAACCGGCCCAATTACCTGTACAGGATGCTGCGCTCTCTGCT CTCAGCCCAAGGGGTATCTCCACAGATGATAACGGTCTTCATTGATGGCTACTATGAGGAGCCAATGGAT GTGGTGGCGCTGTTTGGTCTGAGAGGCATCCAGCATACTCCTATCAGCATCAAGAACGCCCGTGTGTCTC AGCACTACAAGGCCAGCCTCACTGCCACTTTCAACCTGTTTCCGGAGGCCAAGTTTGCTGTGGTTCTGGA AGAGGACCTGGACATTGCCGTGGATTTTTTCAGTTTCCTGAGCCAGTCCATCCACCTGCTGGAGGAAGAT GACAGCCTGTACTGCATCTCTGCCTGGAATGACCAGGGGTATGAACACACAGCTGAGGATCCAGCACTAC TGTACCGTGTGGAGACCATGCCTGGGCTAGGATGGGTGCTCAGGAAATCTTTATACAAGGAGGAGCTTGA GCCCAAGTGGCCCACACCAGAAAAGCTGTGGGATTGGGACATGTGGATGCGGATGCCGGAACAGCGCCGG GGCCGAGAGTGTATCATCCCGGATGTTTCCCGATCCTACCACTTTGGCATCGTCGGCCTCAACATGAACG GCTACTTTCATGAGGCCTACTTCAAGAAGCACAAGTTCAACACAGTTCCAGGTGTCCAGCTCAGGAATGT AGACAGTCTGAAGAAAGAAGCTTATGAAGTGGAAATTCACAGACTGCTCAGTGAGGCCGAGGTTTTGGAC CACAGCAAGGACCCTTGTGAAGACTCCTTCCTGCCAGACACGGAGGGCCACACCTATGTGGCGTTTATCC GAATGGAGACAGATGATGACTTCGCCACCTGGACTCAGCTTGCCAAGTGCCTCCATATTTGGGACCTGGA TGTTCGTGGCAACCACCGGGGCCTGTGGAGATTATTTCGGAAGAAGAACCACTTTTTGGTGGTGGGGGTC CCAGCCTCCCCTTACTCAGTGAAGAAGCCACCATCAGTCACCCCTATTTTCCTGGAGCCACCCCCAAAGG AAGAAGGAGCCCCAGGGGCTGCTGAACAAACGTGAAGCCTCCTCCAGGACTCTGCAGGGCTGGGTACTGT GTACCCCCAGACAGGCTAGCCCGTTGCCCTACCCTGTAGGATTTAGTAGATGCTGGTAGGGGTAGGGCTG GTTTGTTTTTAACATGGAATTTAATTACTAACTCCAAGGGGAAGGATCCCCTGCCCCAACACCCCTATTC CTGAGTTTAAGGTCTATTTATTTACTTCCTTGTCAAAGAAGAGCAGGAGAGTTCCCAGGACCTCTTTGGG ATCCCTGGGTAGCTGATCCACTCTTTTCATACCCTGTCCTCCCAGGCCTGGCTCAGAACCTATTTATTGA CTGTCCTGGGGGCCTTGGGAATAGGCCCAACCCGGGGCCTTTATTCCTCTCTGGACCAGGATGCTGCCCT GAGGGTGGGACTGGCTCATACTCAGGAAAACTGTTGTGCCCAACTGATGGACAGGCCCATATGGGGTCCA TGTGCTGACAGACTCACACAGAGACCTTTAGACACTGGACCAGGTCTCCTCTTAGCCTTCTCTTTGTCCA GATTTCCAAAACTGGATAAAGATGGTCATTGATTAAAAAAGAAGAAAACCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4190368 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4190368 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11514 same embryo
	EMAGE:11513 same embryo
	EMAGE:11516 same embryo
	EMAGE:11517 same embryo
	EMAGE:11512 same embryo
	EurExpress:euxassay_006877 same experiment
	MGI:4827329 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source