EMAGE mouse gene expression spatial database

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EMAGE:11517

Myc myelocytomatosis oncogene ( MGI:97250)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006870. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006870_01	euxassay_006870_02	euxassay_006870_03	euxassay_006870_04

euxassay_006870_05	euxassay_006870_06	euxassay_006870_07	euxassay_006870_08	euxassay_006870_09

euxassay_006870_10	euxassay_006870_11	euxassay_006870_12	euxassay_006870_13	euxassay_006870_14

euxassay_006870_15	euxassay_006870_16	euxassay_006870_17	euxassay_006870_18	euxassay_006870_19

euxassay_006870_20	euxassay_006870_21	euxassay_006870_22	euxassay_006870_23	euxassay_006870_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11517_wholemount_strong.wlz

11517_wholemount_moderate.wlz

11517_wholemount_weak.wlz

11517_wholemount_possible.wlz

11517_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11517_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

strong

regional

strong expression: see section 03 04 05 06 07 08 19 20 21 22 23

thymus primordium

strong

regional

strong expression: see section 11 12 13 14 15 16

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 17 18 19 20

pancreas

moderate

regional

moderate expression: see section 08 09 10 11 12 15 16 17

vibrissa

weak

regional

weak expression: see section 04 05 06 07 08 21 22 23 24

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 14 15 16 17 18 weak expression: see section 06 07 08 09 10 11 12 13 19 20

corpus striatum

moderate

regional

moderate expression: see section 02 03 04 05 06 18 19 20 21 weak expression: see section 01 22

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 11 15 16 17 weak expression: see section 09 10 12

anterior naris

moderate

regional

moderate expression: see section 12 13 14 16 17 18

external naris

moderate

regional

moderate expression: see section 12 13 18

nasal septum

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18

midgut

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

mandible

strong

regional

strong expression: see section 23 24 moderate expression: see section 02 08 09 10 16 17 18 19 20 21 22 weak expression: see section 03 04 05 06 07

maxilla

strong

regional

strong expression: see section 23 24 moderate expression: see section 08 09 10 16 17 18 19 20 21 weak expression: see section 03 04 05 06 07

palatal shelf

moderate

regional

moderate expression: see section 09 10 16 17 18 19

renal cortex

moderate

regional

moderate expression: see section 07 08 09 10 11 17 18 19 20 21 22

frontal bone primordium

strong

regional

strong expression: see section 01 24

parietal bone

strong

regional

strong expression: see section 01 22 23 24

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 07 20 21 22 23 24

clavicle

moderate

regional

moderate expression: see section 09 10 11 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6832
Entity Detected:	Myc, myelocytomatosis oncogene ( MGI:97250)
Sequence:	sense strand is shown >T6832 GATTGGGGTACGCGCTGCGCCAGGTTTCCGCACCAACCAGAGCTGGATAACTCTAGACTTGCTTCCCTTG CTGTGCCCCCTCCAGCAGACAGCCACGACGATGCCCCTCAACGTGAACTTCACCAACAGGAACTATGACC TCGACTACGACTCCGTACAGCCCTATTTCATCTGCGACGAGGAAGAGAATTTCTATCACCAGCAACAGCA GAGCGAGCTGCAGCCGCCCGCGCCCAGTGAGGATATCTGGAAGAAATTCGAGCTGCTTCCCACCCCGCCC CTGTCCCCGAGCCGCCGCTCCGGGCTCTGCTCTCCATCCTATGTTGCGGTCGCTACGTCCTTCTCCCCAA GGGAAGACGATGACGGCGGCGGTGGCAACTTCTCCACCGCCGATCAGCTGGAGATGATGACCGAGTTACT TGGAGGAGACATGGTGAACCAGAGCTTCATCTGCGATCCTGACGACGAGACCTTCATCAAGAACATCATC ATCCAGGACTGTATGTGGAGCGGTTTCTCAGCCGCTGCCAAGCTGGTCTCGGAGAAGCTGGCCTCCTACC AGGCTGCGCGCAAAGACAGCACCAGCCTGAGCCCCGCCCGCGGGCACAGCGTCTGCTCCACCTCCAGCCT GTACCTGCAGGACCTCACCGCCGCCGCGTCCGAGTGCATTGACCCCTCAGTGGTCTTTCCCTACCCGCTC AACGACAGCAGCTCGCCCAAATCCTGTACCTCGTCCGATTCCACGGCCTTCTCTCCTTCCTCGGACTCGC TGCTGTCCTCCGAGTCCTCCCCACGGGCCAGCCCTGAGCCCCTAGTGCTGCATGAGGAGACACCGCCCAC CACCAGCAGCGACTCTGAAGAAGAGCAAGAAGATGAGGAAGAAATTGATGTGGTGTCTGTGGAGAAGAGG CAAACCCCTGCCAAGAGGTCGGAGTCGGGCTCATCTCCATCCCGAGGCCACAGCAAACCTCCGCACAGCC CACTGGTCCTCAAGAGGTGCCACGTCTCCACTCACCAGCACAACTACGCCGCACCCCCCTCCACAAGGAA GGACTATCCAGCTGCCAAGAGGGCCAAGTTGGACAGTGGCAGGGTCCTGAAGCAGATCAGCAACAACCGC AAGTGCTCCAGCCCCAGGTCCTCAGACACGGAGGAAAACGACAAGAGGCGGACACACAACGTCTTGGAAC GTCAGAGGAGGAACGAGCTGAAGCGCAGCTTTTTTGCCCTGCGTGACCAGATCCCTGAATTGGAAAACAA CGAAAAGGCCCCCAAGGTAGTGATCCTCAAAAAAGCCACCGCCTACATCCTGTCCATTCAAGCAGACGAG CACAAGCTCACCTCTGAAAAGGACTTATTGAGGAAACGACGAGAACAGTTGAAACACAAACTCGAACAGC TTCGAAACTCTGGTGCATAAACTGACCTAACTCGAGGAGGAGCTGGAATCTCTCGTGAGAGTAAGGAGAA CGGTTCCTTCTGACAGAACTGATGCGCTGGAATTAAAATGCATGCTCAAAGCCTAACCTCACAACCTTGG CTGGGGCTTTGGGACTGTAAGCTTCAGCCATAATTTTAACTGCCTCAAACTTAAATAGTATAAAAGAACT TTTTTTTATGCTTCCCATCTTTTTTCTTTTTCCTTTTAACAGATTTGTATTTAATTGTTTTTTTAAAAAA ATCTTAAAATCTATCCAATTTTCCCATGTAAATAGGGCCTTGAAATGTAAATAACTTTAATAAAACGTTT ATAACAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3962047 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3962047 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11514 same embryo
	EMAGE:11513 same embryo
	EMAGE:11516 same embryo
	EMAGE:11515 same embryo
	EMAGE:11512 same embryo
	EurExpress:euxassay_006870 same experiment
	MGI:4826532 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source