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EMAGE:11517

Myc myelocytomatosis oncogene ( MGI:97250)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11517 EMAGE:11517 EMAGE:11517 EMAGE:11517 EMAGE:11517
"Pseudo-wholemount" of euxassay_006870. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006870_01 euxassay_006870_02 euxassay_006870_03 euxassay_006870_04
EMAGE:11517 EMAGE:11517 EMAGE:11517 EMAGE:11517 EMAGE:11517
euxassay_006870_05 euxassay_006870_06 euxassay_006870_07 euxassay_006870_08 euxassay_006870_09
EMAGE:11517 EMAGE:11517 EMAGE:11517 EMAGE:11517 EMAGE:11517
euxassay_006870_10 euxassay_006870_11 euxassay_006870_12 euxassay_006870_13 euxassay_006870_14
EMAGE:11517 EMAGE:11517 EMAGE:11517 EMAGE:11517 EMAGE:11517
euxassay_006870_15 euxassay_006870_16 euxassay_006870_17 euxassay_006870_18 euxassay_006870_19
EMAGE:11517 EMAGE:11517 EMAGE:11517 EMAGE:11517 EMAGE:11517
euxassay_006870_20 euxassay_006870_21 euxassay_006870_22 euxassay_006870_23 euxassay_006870_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11517Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11517_wholemount_strong.wlz
11517_wholemount_moderate.wlz
11517_wholemount_weak.wlz
11517_wholemount_possible.wlz
11517_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11517_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
axial musculature
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 19 20 21 22 23
thymus primordium
strong strong
regionalstrong expression: see section 11 12 13 14 15 16
submandibular gland primordium
strong strong
regionalstrong expression: see section 07 08 09 10 17 18 19 20
pancreas
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 15 16 17
vibrissa
weak weak
regionalweak expression: see section 04 05 06 07 08 21 22 23 24
cerebral cortex marginal layer
moderate moderate
regionalmoderate expression: see section 14 15 16 17 18 weak expression: see section 06 07 08 09 10 11 12 13 19 20
corpus striatum
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 18 19 20 21 weak expression: see section 01 22
olfactory cortex marginal layer
moderate moderate
regionalmoderate expression: see section 11 15 16 17 weak expression: see section 09 10 12
anterior naris
moderate moderate
regionalmoderate expression: see section 12 13 14 16 17 18
external naris
moderate moderate
regionalmoderate expression: see section 12 13 18
nasal septum
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17 18
midgut
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18
mandible
strong strong
regionalstrong expression: see section 23 24 moderate expression: see section 02 08 09 10 16 17 18 19 20 21 22 weak expression: see section 03 04 05 06 07
maxilla
strong strong
regionalstrong expression: see section 23 24 moderate expression: see section 08 09 10 16 17 18 19 20 21 weak expression: see section 03 04 05 06 07
palatal shelf
moderate moderate
regionalmoderate expression: see section 09 10 16 17 18 19
renal cortex
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 17 18 19 20 21 22
frontal bone primordium
strong strong
regionalstrong expression: see section 01 24
parietal bone
strong strong
regionalstrong expression: see section 01 22 23 24
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 20 21 22 23 24
clavicle
moderate moderate
regionalmoderate expression: see section 09 10 11 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6832
Entity Detected:Myc, myelocytomatosis oncogene ( MGI:97250)
Sequence:sense strand is shown

>T6832
GATTGGGGTACGCGCTGCGCCAGGTTTCCGCACCAACCAGAGCTGGATAACTCTAGACTTGCTTCCCTTG
CTGTGCCCCCTCCAGCAGACAGCCACGACGATGCCCCTCAACGTGAACTTCACCAACAGGAACTATGACC
TCGACTACGACTCCGTACAGCCCTATTTCATCTGCGACGAGGAAGAGAATTTCTATCACCAGCAACAGCA
GAGCGAGCTGCAGCCGCCCGCGCCCAGTGAGGATATCTGGAAGAAATTCGAGCTGCTTCCCACCCCGCCC
CTGTCCCCGAGCCGCCGCTCCGGGCTCTGCTCTCCATCCTATGTTGCGGTCGCTACGTCCTTCTCCCCAA
GGGAAGACGATGACGGCGGCGGTGGCAACTTCTCCACCGCCGATCAGCTGGAGATGATGACCGAGTTACT
TGGAGGAGACATGGTGAACCAGAGCTTCATCTGCGATCCTGACGACGAGACCTTCATCAAGAACATCATC
ATCCAGGACTGTATGTGGAGCGGTTTCTCAGCCGCTGCCAAGCTGGTCTCGGAGAAGCTGGCCTCCTACC
AGGCTGCGCGCAAAGACAGCACCAGCCTGAGCCCCGCCCGCGGGCACAGCGTCTGCTCCACCTCCAGCCT
GTACCTGCAGGACCTCACCGCCGCCGCGTCCGAGTGCATTGACCCCTCAGTGGTCTTTCCCTACCCGCTC
AACGACAGCAGCTCGCCCAAATCCTGTACCTCGTCCGATTCCACGGCCTTCTCTCCTTCCTCGGACTCGC
TGCTGTCCTCCGAGTCCTCCCCACGGGCCAGCCCTGAGCCCCTAGTGCTGCATGAGGAGACACCGCCCAC
CACCAGCAGCGACTCTGAAGAAGAGCAAGAAGATGAGGAAGAAATTGATGTGGTGTCTGTGGAGAAGAGG
CAAACCCCTGCCAAGAGGTCGGAGTCGGGCTCATCTCCATCCCGAGGCCACAGCAAACCTCCGCACAGCC
CACTGGTCCTCAAGAGGTGCCACGTCTCCACTCACCAGCACAACTACGCCGCACCCCCCTCCACAAGGAA
GGACTATCCAGCTGCCAAGAGGGCCAAGTTGGACAGTGGCAGGGTCCTGAAGCAGATCAGCAACAACCGC
AAGTGCTCCAGCCCCAGGTCCTCAGACACGGAGGAAAACGACAAGAGGCGGACACACAACGTCTTGGAAC
GTCAGAGGAGGAACGAGCTGAAGCGCAGCTTTTTTGCCCTGCGTGACCAGATCCCTGAATTGGAAAACAA
CGAAAAGGCCCCCAAGGTAGTGATCCTCAAAAAAGCCACCGCCTACATCCTGTCCATTCAAGCAGACGAG
CACAAGCTCACCTCTGAAAAGGACTTATTGAGGAAACGACGAGAACAGTTGAAACACAAACTCGAACAGC
TTCGAAACTCTGGTGCATAAACTGACCTAACTCGAGGAGGAGCTGGAATCTCTCGTGAGAGTAAGGAGAA
CGGTTCCTTCTGACAGAACTGATGCGCTGGAATTAAAATGCATGCTCAAAGCCTAACCTCACAACCTTGG
CTGGGGCTTTGGGACTGTAAGCTTCAGCCATAATTTTAACTGCCTCAAACTTAAATAGTATAAAAGAACT
TTTTTTTATGCTTCCCATCTTTTTTCTTTTTCCTTTTAACAGATTTGTATTTAATTGTTTTTTTAAAAAA
ATCTTAAAATCTATCCAATTTTCCCATGTAAATAGGGCCTTGAAATGTAAATAACTTTAATAAAACGTTT
ATAACAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3962047 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3962047 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11514 same embryo
 EMAGE:11513 same embryo
 EMAGE:11516 same embryo
 EMAGE:11515 same embryo
 EMAGE:11512 same embryo
 EurExpress:euxassay_006870 same experiment
 MGI:4826532 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS