EMAGE mouse gene expression spatial database

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EMAGE:11572

Arnt aryl hydrocarbon receptor nuclear translocator ( MGI:88071)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003638. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003638_01	euxassay_003638_02	euxassay_003638_03	euxassay_003638_04

euxassay_003638_05	euxassay_003638_06	euxassay_003638_07	euxassay_003638_08	euxassay_003638_09

euxassay_003638_10	euxassay_003638_11	euxassay_003638_12	euxassay_003638_13	euxassay_003638_14

euxassay_003638_15	euxassay_003638_16	euxassay_003638_17	euxassay_003638_18	euxassay_003638_19

euxassay_003638_20	euxassay_003638_21	euxassay_003638_22	euxassay_003638_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11572_wholemount_strong.wlz

11572_wholemount_moderate.wlz

11572_wholemount_weak.wlz

11572_wholemount_possible.wlz

11572_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11572_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6856
Entity Detected:	Arnt, aryl hydrocarbon receptor nuclear translocator ( MGI:88071)
Sequence:	sense strand is shown >T6856 GCGGCGGTAAGGTGCCTAATCTGCGGAGTGGCTCTTCCCTCCCCTCCCCCAGCTCGGTGGCGGCTGCCCC TCCCACCGAGGGTGGCGCAGGGACGGTGCCATCTCGACCATGGCGGCGACTACAGCTAACCCAGAAATGA CATCAGATGTACCATCGCTGGGTCCCACCATTGCTTCTGGAAACCCTGGACCTGGGATTCAAGGTGGAGG AGCTGTTGTACAGAGGGCTATTAAGCGACGGTCAGGGCTGGATTTTGATGATGAAGTAGAAGTGAACACT AAATTTTTGAGATGCGATGATGACCAGATGTGTAATGACAAAGAGCGGTTTGCCAGGTCGGATGATGAGC AGAGCTCTGCGGATAAAGAGAGACTTGCCAGGGAAAATCATAGTGAAATAGAACGGCGGCGACGGAACAA GATGACAGCTTACATCACAGAACTGTCAGACATGGTACCTACATGTAGTGCCCTGGCTCGAAAACCAGAC AAGCTAACCATCTTACGCATGGCCGTTTCTCACATGAAGTCCTTGAGGGGAACTGGCAACACATCTACTG ATGGCTCCTACAAGCCATCTTTCCTCACTGATCAGGAACTGAAACATTTGATCTTGGAGGCAGCAGATGG CTTTCTGTTTATTGTCTCCTGTGAGACTGGACGGGTGGTGTATGTCTCTGACTCAGTGACTCCCGTTTTG AACCAGCCACAGTCTGAATGGTTCGGGAGCACACTGTATGATCAGGTGCACCCAGATGATGTGGATAAAC TTCGAGAGCAGCTCTCTACATCAGAAAATGCCCTAACAGGGCGGGTCCTGGATCTGAAGACTGGAACAGT GAAAAAGGAAGGCCAGCAGTCTTCCATGAGGATGTGCATGGGCTCACGAAGGTCGTTCATCTGCCGCATG AGGTGTGGTACTAGCTCCGTGGACCCTGTTTCCATGAATAGACTGAGCTTTTTGAGGAACAGATGCAGGA ATGGGCTTGGCTCTGTGAAGGAAGGAGAACCTCACTTTGTGGTAGTCCACTGCACAGGCTACATCAAGGC CTGGCCACCAGCAGGTGTCTCCCTCCCAGATGATGACCCAGAGGCTGGCCAGGGGAGCAAATTCTGCCTA GTGGCCATTGGCAGGCTGCAGGTAACTAGTTCTCCCAACTGTACAGACATGAGTAACATTTGTCAGCCAA CAGAGTTCATCTCCCGACACAACATTGAAGGGATATTCACTTTTGTAGACCATCGTTGTGTGGCTACTGT TGGCTACCAGCCACAGGAGCTCTTAGGGAAGAATATTGTAGAATTTTGTCATCCTGAAGACCAACAACTT CTAAGAGACAGCTTTCAGCAGGTGGTGAAATTAAAAGGTCAGGTGCTGTCCGTCATGTTCCGATTCCGAT CTAAGACCCGAGAATGGCTGTGGATGAGAACGAGCTCCTTTACCTTCCAAAACCCTTATTCAGATGAAAT TGAGTATATTATCTGCACCAACACCAATGTGAAGAACTCTAGCCAGGAACCACGGCCTACACTGTCCAAC ACCATCCCAAGGTCACAGCTAGGTCCGACAGCCAATTTATCCCTAGAGATGGGTACAGGGCAGCTGCCAT CCAGGCAGCAGCAGCAGCAGCACACAGAACTGGATATGGTACCAGGAAGAGATGGGCTGGCCAGCTATAA TCATTCCCAGGTTTCTGTCCAGCCTGTGGCAAGTGCAGGATCAGAACACAGCAAGCCCCTTGAGAAGTCA GAAGGTCTCTTTGCACAGGACAGAGATCCAAGGTTTCCAGAAATCTATCCCAGCATCACTGCAGATCAGA GTAAAGGCATCTCCTCCAGCACTGTCCCTGCCACCCAACAGCTGTTCTCCCAGGGCAGCTCATTCCCTCC TAACCCCCGGCCGGCAGAGAATTTCAGGAATAGTGGTCTTACCCCTCCTGTAACCATTGTCCAGCCATCA TCTTCTGCAGGGCAGATACTGGCCCAGATTTCACGTCACTCCAACCCTGCCCAGGGATCAGCGCCGACCT GGACCTCTAGCTCCCGCCCAGGCTTTGCCGCCCAGCAGGTGCCCACCCAGGCTACAGCCAAGACTCGTTC TTCCCAATTTGGTGTGAACAACTTTCAGACTTCTTCCTCCTTCAGTGCTATGTCTCTTCCGGGTGCTCCC ACTGCCTCATCTGGTACTGCTGCCTACCCTGCTCTCCCCAACCGTGGCTCCAACTTTCCTCCTGAGACTG GACAGACCACAGGACAGTTCCAGGCCCGGACAGCAGAGGGCGTGGGTGTCTGGCCACAGTGGCAGGGCCA GCAGCCCCATCATCGGTCTAGTTCCAGTGAGCAGCATGTTCAGCAGACACAAGCACAAGCACCTAGCCAG CCTGAGGTCTTTCAAGAAATGCTGTCCATGCTGGGAGACCAAAGCAACACCTACAACAATGAAGAATTTC CTGATCTAACTATGTTTCCCCCCTTTTCCGAATAGAACTATTGGGGTGAGGATAAGGGTGGGGGGAAATC ACTGTTTGTTTTTAAAAGCAAATCTTTTGTAAACAGAATAAAAGTTCCTCTCCCTTCCCTTCCCTCACCC CTGATATGTACCCTTTCCACCCCTTGACTTGCTGAAGAAACGTTATAGAAGAAATTAAATGAATTTCCCA GGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3964932 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3964932 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11573 same embryo
	EMAGE:11574 same embryo
	EMAGE:11577 same embryo
	EMAGE:11576 same embryo
	EMAGE:11575 same embryo
	EurExpress:euxassay_003638 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source