EMAGE mouse gene expression spatial database

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EMAGE:11574

Cib2 calcium and integrin binding family member 2 ( MGI:1929293)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003639. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003639_01	euxassay_003639_02	euxassay_003639_03	euxassay_003639_04

euxassay_003639_05	euxassay_003639_06	euxassay_003639_07	euxassay_003639_08	euxassay_003639_09

euxassay_003639_10	euxassay_003639_11	euxassay_003639_12	euxassay_003639_13	euxassay_003639_14

euxassay_003639_15	euxassay_003639_16	euxassay_003639_17	euxassay_003639_18	euxassay_003639_19

euxassay_003639_20	euxassay_003639_21	euxassay_003639_22	euxassay_003639_23	euxassay_003639_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11574_wholemount_strong.wlz

11574_wholemount_moderate.wlz

11574_wholemount_weak.wlz

11574_wholemount_possible.wlz

11574_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11574_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 12 14 16 17

pons mantle layer

moderate

regional

moderate expression: see section 07 18

ventral grey horn

moderate

regional

moderate expression: see section 11 12 15 16 weak expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6634
Entity Detected:	Cib2, calcium and integrin binding family member 2 ( MGI:1929293)
Sequence:	sense strand is shown >T6634 CGGACGCGTGGGCCGGGCTCGGGGCTCCAGACGCCGCCGATGGGAAGCAGCACTCGAAAGGGCGAGAGAC GACGCCGCGGCCACCCATACCATCGCGGTCACCATGGGGAACAAGCAGACCATCTTCACTGAAGAGCAGC TGGACAACTACCAGGACTGCACTTTCTTCAATAAGAAGGACATCCTCAAGCTTCATGCACGGTTCTATGA GCTGGCTCCCAACCTCGTCCCGATGGACTACAGGAAGAGTCCCATCGTCCATGTACCCATGAGCCTCATC ATTCAGATGCCGGAGCTCCGGGAGAATCCCTTCAAAGAGAGGATTGTGGAGGCTTTCTCCGAGGATGGCG AGGGGAACCTCACCTTCAATGACTTTGTGGACATGTTCTCTGTGCTCTGCGAATCAGCGCCTCGGGAGCT CAAGGCAAACTATGCCTTCAAGATCTATGACTTCAACACTGACAATTTTATCTGTAAAGAAGACTTAGAG ATGACGCTGGCCCGACTCACCAAGTCTGAGTTGGAAGAGGATGAGGTAGTGCTTGTGTGTGACAAAGTCA TTGAAGAGGCTGACCTGGATGGTGACGGCAAGCTGGGCTTTGCTGACTTTGAGGACATGATCGCCAAGGC CCCTGATTTTCTCAGCACCTTCCACATTCGAATCTGAAGACACTGCCAAGGCCGTGGGGGCCTAGAGCTG CATCCTCTGGCCTGCTGTCTGTGCAGGTATGGTTTCCAAGTGCTCCAGGGAAGGAGCTGTGGCCTCTGGG GTTCTCACCCATGGACATTTCCTGTGGCCCTTTCAGCAAAAAAATTAAAATAAAATAAAATAAAATAAAA TAAAATAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3599342 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3599342 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11573 same embryo
	EMAGE:11572 same embryo
	EMAGE:11577 same embryo
	EMAGE:11576 same embryo
	EMAGE:11575 same embryo
	EurExpress:euxassay_003639 same experiment
	MGI:4823875 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source