EMAGE mouse gene expression spatial database

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EMAGE:11577

Gja1 gap junction protein, alpha 1 ( MGI:95713)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003641. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003641_01	euxassay_003641_02	euxassay_003641_03	euxassay_003641_04

euxassay_003641_05	euxassay_003641_06	euxassay_003641_07	euxassay_003641_08	euxassay_003641_09

euxassay_003641_10	euxassay_003641_11	euxassay_003641_12	euxassay_003641_13	euxassay_003641_14

euxassay_003641_15	euxassay_003641_16	euxassay_003641_17	euxassay_003641_18	euxassay_003641_19

euxassay_003641_20	euxassay_003641_21	euxassay_003641_22	euxassay_003641_23	euxassay_003641_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11577_wholemount_strong.wlz

11577_wholemount_moderate.wlz

11577_wholemount_weak.wlz

11577_wholemount_possible.wlz

11577_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11577_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6632
Entity Detected:	Gja1, gap junction protein, alpha 1 ( MGI:95713)
Sequence:	sense strand is shown >T6632 CAGCACTTTTCTTTCATTGGGGGAAAGGCGTGAGGGAAGTACCCAACAGCAGCAGACTTTGAAACTTTAA ACAGACAGGTCTGAGAGCCCGAACTCTCCTTTTCCTTTGACTTCAGCCTCCAAGGAGTTCCACCACTTTG GCGTGCCGGCTTCACTTTCATTAAGTGAAAGAGAGGTGCCCAGACATGGGTGACTGGAGCGCCTTGGGGA AGCTGCTGGACAAGGTCCAAGCCTACTCCACGGCCGGAGGGAAGGTGTGGCTGTCGGTGCTCTTCATTTT CAGAATCCTGCTCCTGGGGACAGCGGTTGAGTCAGCTTGGGGTGATGAACAGTCTGCCTTTCGCTGTAAC ACTCAACAACCCGGTTGTGAAAATGTCTGCTATGACAAGTCCTTCCCCATCTCTCACGTGCGCTTCTGGG TCCTTCAGATCATATTCGTGTCTGTGCCCACACTCCTGTACTTGGCTCACGTGTTCTATGTGATGAGAAA GGAAGAGAAGCTGAACAAGAAAGAAGAGGAGCTCAAAGTGGCGCAGACCGACGGGGTCAACGTGGAGATG CACCTGAAGCAGATTGAAATCAAGAAGTTCAAGTATGGGATTGAAGAACACGGCAAGGTGAAGATGAGAG GTGGCCTGCTGAGAACCTACATCATCAGCATCCTCTTCAAGTCTGTCTTCGAGGTGGCCTTCCTGCTGAT CCAGTGGTACATCTATGGGTTCAGCCTGAGTGCGGTCTACACCTGCAAGAGAGATCCCTGCCCCCACCAG GTGGACTGCTTCCTCTCACGTCCCACGGAGAAAACCATCTTCATCATCTTCATGCTGGTGGTGTCCTTGG TGTCTCTCGCTCTGAATATCATTGAGCTCTTCTATGTCTTCTTCAAGGGCGTTAAGGATCGCGTGAAGGG AAGAAGCGATCCTTACCACGCCACCACCGGCCCACTGAGCCCATCCAAAGACTGCGGATCTCCAAAATAT GCTTACTTCAATGGCTGCTCCTCACCAACGGCCCCACTCTCACCTATGTCTCCTCCTGGGTACAAGCTGG TCACTGGTGACAGAAACAATTCCTCCTGCCGCAATTACAACAAGCAAGCCAGCGAGCAAAACTGGGCGAA TTACAGCGCAGAGCAAAATCGAATGGGGCAGGCCGGAAGCACCATCTCCAACTCCCACGCCCAGCCGTTT GATTTCCCTGACGACAGCCAAAATGCCAAAAAAGTTGCTGCTGGACACGAACTCCAGCCCTTAGCTATCG TGGATCAGCGACCTTCCAGCAGAGCCAGCAGCCGCGCCAGCAGCAGACCTCGGCCTGATGACCTGGAGAT TTAAACAGGCTTGAACATCAAGCTGCCAATCGATTGTGGAGGAGAAAAAAAAGGGTGCTTGCAGAACGTG CACCTGGGGTGTTCATTTCGTTCCCGTGGAGGTGGTACTCAACAACCTCAGTAATGAGGCGTAGAAAACA AAGACATTACAATATCTAGGTTCCTTGGGGGGTGTTTTGGGATAGCTAGGCGGCAAAAGTAGGGAAAGGG GAGGTATGTAACGGTATTTAATGTAGAAGATTCAAAGAGCTTAAATTCTAGTAAGAGTCTCATTGGATGA AACATAGATAGGGCTTTCTCTCTCTGCCCCCCAACTGAACCTTAAGAATGGTTCTGTATACATGAGTGAG TGGGTGATATATATTTTTTTTAATTTTTGTTTTACTGAGATTCTGCCATAGAGCTTTGAGCAGGAATCCA AGTCCTCAACATGGCATTTCCTTTATGAAAAGACAGGTTGTCCTACATCCCCGCTAAAAAACATTCCAGT GTTTAAAAACTTGGCAGTTTGCAGGCGAGCTTCCCTGGCCTGACCCTCTAGGTGTGGATGGACCTTATGC TACTATACACGATTTTCATTCTTGGTAGGTATCAATTCGAAGTTCAGACAAGGTTCAAAGAAAAAGATTG CCCATGTATTTGCATCTCAGTGGGTTCTTTTTCAAATCTGTCCCACCTTTGTGTCTTCCATATATTATCC TCAGCTGGTCCTCACCCTCACCAAATGATTTCTATCGACATTTTTAAAACAGTGAGAAAGTCTTTTTTTT TTTTTTTTGAGTTAGCATCAGGGAGGCAAGCCATGCTCAATATTTAACAATCGCTTCTGTCTATGTGTGG GTGTGCAAGTGTGTAAGCGTGTGTTTTGTCATTATTGGTACAAGCAGAGGCAGTATAAACTCACAGATTT GAATCGAATTCACACAGTGTTCAAATTTGAACCTTCCTCATGGATCTTTGTGGTGTGGGCCAACGTGGTG TTTACATTATAGAATTCCTGCCGTGCAAAAGTGTAAAGCACACACTTTTTCCCTAAAATATTTTTTCCAC GTATCCTATTATGGATACTGGTTTTGTTAATTATGATTTTTTTTTCTTTTTTAGAATGTAGCAGTAATAG CCATTACTGAAATGAATGATTTCCTTTTTCTGAAATATAATCATTGATGCTTGAATGATAGAATTTTAGT ACTGTAAACAGGCTTTAGTCATTAATGTGAGAGACTTAGAAGAGGGTTGCTTAGAGTGGACTATCAAGTG AGCCTAAAGGAACTTTGTAGTAACTGGTAATCTGGTAATTTTTGTCCTACTTAACTACACATTAACTCAG AACTTGTATTCTGAGTTTAACAGTCTTTTAGATTGACGAGCAACTTGGATGTTTGCACTAAGATTTTCTT TGAGATACTAGAGGGGGTGAAGGAGTTTTCAGCAGTGCACATGTAACTAATTTATTTGAACTGTAAGCTA AAGACACCTACCAGTTTCTTCAAGTGACTTAAAAAAACTCATCACAGATGATTGAAATGTCGAGTTATCA TGTTTCCTCTTGCGCGCCAGCTACACAAGGAGTTTTTGGACAATGAGAAACTAATTTGTTTGACATTCCA TGTTAAACTACTGTCATGTTCAGCTTCATTGCATGTAATGTAGACCTAGCCCATCCAATCAATGTGCTCG GGAAAGTGTTCTTTATTCAATAAAATTTTAATTTAGTATAATAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3599347 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3599347 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11573 same embryo
	EMAGE:11572 same embryo
	EMAGE:11574 same embryo
	EMAGE:11576 same embryo
	EMAGE:11575 same embryo
	EurExpress:euxassay_003641 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source