EMAGE mouse gene expression spatial database

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EMAGE:11684

Pycr1 pyrroline-5-carboxylate reductase 1 ( MGI:2384795)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006989. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006989_01	euxassay_006989_02	euxassay_006989_03	euxassay_006989_04

euxassay_006989_05	euxassay_006989_06	euxassay_006989_07	euxassay_006989_08	euxassay_006989_09

euxassay_006989_10	euxassay_006989_11	euxassay_006989_12	euxassay_006989_13	euxassay_006989_14

euxassay_006989_15	euxassay_006989_16	euxassay_006989_17	euxassay_006989_18	euxassay_006989_19

euxassay_006989_20	euxassay_006989_21	euxassay_006989_22	euxassay_006989_23	euxassay_006989_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11684_wholemount_strong.wlz

11684_wholemount_moderate.wlz

11684_wholemount_weak.wlz

11684_wholemount_possible.wlz

11684_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11684_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 21 22 23 24

otic capsule

strong

regional

strong expression: see section 07 08 09 10 16 17 18

nasal septum

strong

regional

strong expression: see section 14

viscerocranium

strong

regional

Expression in the turbinate bone.

mandible

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 17 18 19 20 21 22 23

upper jaw skeleton

strong

regional

strong expression: see section 05

maxilla

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 16 17 18 19 20 21 22 23

axial skeleton

strong

regional

strong expression: see section 13 14 15

temporal bone petrous part

strong

regional

strong expression: see section 03 04 21 22 23 24

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 22 23 24

clavicle

strong

regional

strong expression: see section 05 06 07 08 09 10 11 17 18 19 20 21 22 23

tail paraxial mesenchyme

strong

regional

strong expression: see section 10 11 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6830
Entity Detected:	Pycr1, pyrroline-5-carboxylate reductase 1 ( MGI:2384795)
Sequence:	sense strand is shown >T6830 CGGACGCGTGGGCCCGAATATCCACGCTTTCTCTGCTGCCCTACAGAGCCGGCGTCAGCTGTGAAAGTTG TGGGTCGTGGACCTCCAGACATGAGCGTAGGCTTCATCGGGGCAGGGCAGCTAGCCTTTGCTCTAGCCAA GGGCTTCACAGCTGCAGGTGTTCTGGCTGCACACAAGATAATGGCCAGCTCCCCAGACATGGACCAAGCT ACGGTCTCGGCCCTCCGGAAGATAGGGGTGAACCTGACACCCCACAACAAAGAGACTGTGCGCCACAGTG ATGTGCTCTTCCTGGCTGTGAAGCCACACATCATCCCCTTCATCCTGGATGAAATTGGGGCGAACATTGA GGACAGGCACATTGTGGTGTCTTGTGCGGCAGGGGTCACCATCAACTCCATCGAAAAGAAGCTGACAGCG TTCCAACCTGCCCCCAAAGTCATCCGATGTATGACTAACACCCCGGTCGTGGTACGGGAGGGGGTCACTG TGTATGCCACAGGTACTCACGCTCAGGTGGAAGATGGCAGGCTTGTGGAGCAGCTCATGGGCAGCGTGGG CTTCTGCACAGAGGTGGAGGAAGATCTGATCGATGCTGTCACAGGGCTCAGTGGCAGCGGCCCTGCCTAT GCTTTCACAGCCCTGGATGCTCTGGCTGACGGTGGTGTGAAAATGGGGCTTCCCAGACGCCTGGCAGTCC GCCTCGGGGCCCAGGCCCTCCTGGGGGCAGCCAAGATGCTACTAGACTCAGAACAGCATCCCAGCCAGCT CAAGGATAATGTCTGCTCTCCGGGTGGGGCCACCATCCATGCCTTGCATGTGCTGGAGAGCGGGGGCTTT CGCTCCTTGCTTATCAACGCTGTGGAAGCCTCCTGCATCCGTACACGGGAGCTGCAGACCATGGCTGACC AGGAGACCATCTCTCCTGCTGCCATAAAGAAGACTGTACTGGATAAGGTGAAGCTGGACTCCTCTGCTGG GGCCTCTCTGTCTTCTGACCATGTCAAGCCACTGCCCTGAAACCTGGTCCCAGCAGGCAAGGAATGATTT GTCTGCCAGCCTTCCTCCTGTTCCCTACTTTCCTTTCCTTTCCTCTCCTCTCCTCTCCTCTCCTCTCCTC TCCTCTCCTCTCCAGCAGGGTTTTGCAGTGTACTCCAGGTTAGCTCTGAACTAGTCATCCCCCACTCTTG TGTCCCAAGTGCTGGGATCATGGATAAGTGTTAACATGCCAGGATCCTCTTCTATCTTTAAGTCTAAGAT AGTGACTTGATGTTGCCCAGACAAGACTTGAACTTACAGGCTCAGGGCATACTCTGATTTCAATTCCCGT TAGGTACACAACACCTGAGAGACTCAGTTCTTCTCTTTCTTCTTTTCTTGCTTGAAGCTTCTGTCCCAAC GCTCTCAAACAACACCGAATATCCCCATAGCAGGCCCTTGCCCACAGCCAGGTCAGCAAGGAAGGGGATA TCACTTACAACTGGGGACCTCCATTGATACTCAAGTGCCAGCTAGACACTAGGTGGCTTCTCAGACCTCC CCCTCTTCAGCATCCTCTGGAGCTCAGGCCTGTGGGGTCTTTACTCTCCCTAGTTTCAGGGTCAGCTAGC TAGTTAGCCATGGCCTCTCCCTTTCTGTGATGACCAGCTGGCCCAAGACATCGTGTACCTCCTTCTAGGG ACTTAACGCTTTAGCAATGATTCCCTAGGCACTGGGGAAAAGAACTTCTCGGGTAGCTGACACGACTCCT GCATCTTCCCCAACTCACCAAACTGCTACTGCTCTTCCTATCCTTTCAGTTGAATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3962093 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3962093 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11683 same embryo
	EMAGE:11680 same embryo
	EMAGE:11681 same embryo
	EMAGE:11682 same embryo
	EurExpress:euxassay_006989 same experiment
	MGI:4827551 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source