EMAGE mouse gene expression spatial database

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EMAGE:11749

Plat plasminogen activator, tissue ( MGI:97610)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007125. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007125_01	euxassay_007125_02	euxassay_007125_03	euxassay_007125_04

euxassay_007125_05	euxassay_007125_06	euxassay_007125_07	euxassay_007125_08	euxassay_007125_09

euxassay_007125_10	euxassay_007125_11	euxassay_007125_12	euxassay_007125_13	euxassay_007125_14

euxassay_007125_15	euxassay_007125_16	euxassay_007125_17	euxassay_007125_18	euxassay_007125_19

euxassay_007125_20	euxassay_007125_21	euxassay_007125_22	euxassay_007125_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11749_wholemount_strong.wlz

11749_wholemount_moderate.wlz

11749_wholemount_weak.wlz

11749_wholemount_possible.wlz

11749_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11749_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4560
Entity Detected:	Plat, plasminogen activator, tissue ( MGI:97610)
Sequence:	sense strand is shown >T4560 GGAACTCAAGAGCTCAGCGTCAAGGAGAGGCCCCTGCAAGAGTGGGAAAGAAGCAAGCACTCTCGGGACA CAGAAGAAACGGGGAGCAAAATGAAGAGAGAGCTGCTGTGTGTACTGCTGCTTTGTGGACTGGCTTTCCC ATTGCCTGACCAGGGAATACATGGGAGGTTCAGAAGAGGAGCCCGGTCCTACAGAGCGACCTGCAGAGAT GAGCCAACGCAGACAACTTACCAACAGCACCAGTCGTGGCTCCGACCCATGCTCAGAAGCAGCCGGGTGG AATATTGCCGGTGCAACAGCGGCCTGGTACAATGCCACTCAGTGCCTGTCCGAAGTTGCAGCGAACCAAG ATGCTTCAATGGGGGGACGTGTCAGCAGGCCCTGTATTTCTCTGACTTTGTCTGCCAGTGCCCTGATGGA TTTGTAGGGAAACGCTGTGACATAGATACCAGAGCAACATGCTTTGAGGAGCAGGGCATCACCTACAGAG GCACGTGGAGCACAGCAGAAAGTGGGGCTGAGTGCATCAACTGGAATAGCAGTGTTCTGTCGCTGAAGCC CTACAATGCAAGGAGGCCAAATGCCATCAAGCTGGGCCTTGGGAATCACAATTACTGCAGAAACCCAGAC CGAGACTTGAAGCCCTGGTGCTATGTCTTTAAGGCAGGGAAGTATACCACGGAGTTCTGTAGCACACCAG CTTGCCCTAAGGGAAAAAGCGAGGACTGCTATGTTGGGAAAGGTGTGACTTACCGTGGCACCCACAGCCT CACCACATCCCAGGCCTCCTGCCTCCCCTGGAATTCCATAGTCCTCATGGGCAAGAGTTACACAGCGTGG AGGACCAACTCCCAGGCACTCGGCCTGGGCAGACACAATTATTGTCGGAATCCAGATGGTGATGCCAGAC CTTGGTGCCATGTGATGAAGGACCGAAAGCTGACGTGGGAATACTGTGACATGTCCCCATGCTCCACCTG TGGCCTGAGGCAGTACAAACGGCCTCAGTTTAGAATTAAAGGAGGACTCTACACAGACATCACCTCACAC CCTTGGCAGGCTGCCATCTTTGTCAAGAACAAGAGGTCTCCTGGAGAGAGATTCCTTTGTGGAGGGGTGC TGATCAGTTCCTGCTGGGTGCTGTCAGCTGCCCACTGCTTTCTAGAGAGGTTCCCCCCCAATCATCTTAA AGTGGTCTTGGGCAGAACATACAGGGTGGTCCCCGGAGAGGAAGAACAGACATTTGAGATTGAAAAATAC ATAGTCCATGAGGAATTTGATGACGATACTTATGACAACGACATCGCATTACTGCAGCTGAGGTCACAGT CCAAGCAATGTGCCCAAGAGAGCAGCTCTGTTGGCACTGCCTGCCTCCCTGACCCCAACCTGCAGCTCCC TGACTGGACAGAGTGTGAGCTTTCTGGCTACGGCAAGCATGAGGCATCGTCTCCATTCTTCTCTGACCGG CTGAAGGAGGCTCACGTCAGACTGTACCCGTCCAGCCGCTGCACCTCACAGCATCTGTTTAATAAAACCG TCACGAACAACATGCTGTGCGCTGGAGACACCCGGAGCGGAGGCAACCAAGACCTCCACGATGCATGCCA GGGTGACTCGGGAGGCCCTCTGGTGTGCATGATCAATAAACAGATGACATTGACTGGCATTATCAGCTGG GGCCTCGGCTGTGGGCAGAAGGATGTGCCTGGGGTCTACACAAAGGTTACGAATTACCTAGACTGGATTC ACGACAACATGAAGCAATGACAAAGAAAGCCCAGCTCCTTCAATCCAGAGGAGGACCTGCCTTCCTCTTC CTCTTCTACAGAAGATGCGCCTAAAAGGCCAAGCATTCTTTACACCATCATCTTCGTGAGCTGCCACTCG GGAGAGGGGAGGGGGGTCTTTGGGAGCAGATGTAGCATTTACCTGTGACAGGTACTTCACAACTTGTAAG TTTTAAGGATGAAGGTCTGGCTTTAGAATAAATCCTGTCAGATGAGATGACAGGGAGATGCCAACCTTCC TATAACTCTAGGATTTTAAAAAGAGAGGTAGACCAAAGTCTGCCCTCCTGGTCCACTATGTTGTACACTG AACCACAAGATCATGTCTCAACAGCAAAAAAATACAACTTGATCTTTCAGGAGTGAAAGTTTGCACTGGG GACAAGAATGTGTTTTTATAGTTACACAGGGGGCCCCACAGGGCCTCCACGAGAAGGAAGGGGTATCTGG CCGAATCACAGCACCATAAAATCCTTGAGATCATGCACTCCCCATCCCTCCACACTCCTCAACTCTTGGG ACATATCCCTTTGTATACAGTGTAAATGTCTTTTTCTTTATAAACTCTATAGATGGTTGGGAGAACTGTA TGATTTTAATAATTGATGAATAACACTAGTATATTTATATTTTAATCTATTTTAGTTTTTACTTTGTTAC TATAACTTTGTATTATACTGTACTTAAATAATAAATTCAGAGGTATTTTTCACACTTTAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4979664 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4979664 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11754 same embryo
	EMAGE:11750 same embryo
	EMAGE:11752 same embryo
	EMAGE:11753 same embryo
	EMAGE:11751 same embryo
	EurExpress:euxassay_007125 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source