EMAGE mouse gene expression spatial database

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EMAGE:11752

Cxcl5 chemokine (C-X-C motif) ligand 5 ( MGI:1096868)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007109. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007109_01	euxassay_007109_02	euxassay_007109_03	euxassay_007109_04

euxassay_007109_05	euxassay_007109_06	euxassay_007109_07	euxassay_007109_08	euxassay_007109_09

euxassay_007109_10	euxassay_007109_11	euxassay_007109_12	euxassay_007109_13	euxassay_007109_14

euxassay_007109_15	euxassay_007109_16	euxassay_007109_17	euxassay_007109_18	euxassay_007109_19

euxassay_007109_20	euxassay_007109_21	euxassay_007109_22	euxassay_007109_23	euxassay_007109_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11752_wholemount_strong.wlz

11752_wholemount_moderate.wlz

11752_wholemount_weak.wlz

11752_wholemount_possible.wlz

11752_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11752_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 12 13 14 16 17 18

lower jaw molar

strong

regional

strong expression: see section 06 08 17 18 20 21

upper jaw molar

strong

regional

strong expression: see section 05 06 07 08 17 19 20 21

liver left lobe

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11

liver right lobe

strong

spotted

strong expression: see section 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4551
Entity Detected:	Cxcl5, chemokine (C-X-C motif) ligand 5 ( MGI:1096868)
Sequence:	sense strand is shown >T4551 CACACCTCCTCCAGCATATCCCGAGCTGGTGCTCCCTGGTCCGGGATCTTGTCCACAATGAGCCTCCAGC TCCGCAGCTCCGCCCACATCCCCAGCGGTTCCAGCTCGCCATTCATGCGGATGGCGCCGCTGGCATTTCT GTTGCTGTTCACGCTGCCGCAGCATCTAGCTGAAGCTGCCCCTTCCTCAGTCATAGCCGCAACGGAGCTG CGTTGTGTTTGCTTAACCGTAACTCCAAAAATTAATCCCAAATTGATCGCTAATTTGGAGGTGATCCCTG CAGGTCCACAGTGCCCTACGGTGGAAGTCATAGCTAAACTGAAAAACCAGAAGGAGGTCTGTCTGGATCC AGAAGCTCCTGTGATAAAGAAAATCATTCAGAAAATATTGGGCAGTGACAAAAAGAAAGCTAAGCGGAAT GCACTCGCAGTGGAAAGAACGGCCAGTGTTCAATAGAAAGATTTCTGAGGACTCTGACCCCAGTGAAGAG AAGAAAGGGCTGATTCTCTCCACCCACGGATTTTCTTTATGAACTCCCTGCTTTGATGAGAAAAGGGAAA CCATTGTCCCTGAAGCTTCCTGCTCATCTCACAAGTGTATTTAGCAAAAATATTTCCTATTCTTTCCTAT CTGTCACACTATCGGAAATTGTGATACTTTAGTGTGTAAGTCAAGAATCATTGGTTGTTAACCTTTCAAA ATGTCTTGAACTGAAGGTGACTATTGCATTGTCTCCGGAATATGTATATGTATATGTATATGTATATGTA TATGTATATGTATATGTATATGTATATGTGTGTGTATATATATATATATCCTTTGTAATGTTCATGAGAA GGCAATGCTGTCATCCTCTCTCTCATGAAATGGGGACCTCTTTTACTGTTGTTTCTTGGGTGTGTTAAGA GTGTTCTTACGTCTGAGCCTGGGCTGGCATATAACTTTTATGGTTTTAGAAAGCTGCTGTGTCATGCAGA AACCTGTGTAGAATACAGTTCCTTATTTAGAATTTCTAAATGTGTAAGTTCTAGAAGGATCAATGTGTTC TCATCTTATAATTTTAGACATTTGATGTCTTCGTAGTATGGCATAATGTCATGACTTACTCAACAAACTG TATACTATTTATTAATTATTAATTAGTCTATTAGTATTACTATTTGGGCCACTACGTATATATTTTAGAT AGTATATATTTTAGATAGGTGAAGAAGGTAAGAAGCAAGGAAATTCCTTACCTAGTTTTCATAAAAAACA TTTAAAAGTTATTTCTAGGGAGAGAAACTTTAGCAAATATCTATACTCTTAGAGTTTTGAAAACATACTT AACATGTTTGAATATGGATTGCTGGGATATTTAGATGTCTGTAGTGTGTCTTTTAAAGAGTGTGCTTTGT TATAAGGAGATTAATGAATTCATCACATCCATTATTTTAAGCCTGTACATCTTATAGTGTCTCACTTCTT TATATTACTCTTTTGAGGAAAATAAAGTACCTCTAGATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4976854 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4976854 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11754 same embryo
	EMAGE:11750 same embryo
	EMAGE:11753 same embryo
	EMAGE:11749 same embryo
	EMAGE:11751 same embryo
	EurExpress:euxassay_007109 same experiment
	MGI:4824135 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source