EMAGE mouse gene expression spatial database

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EMAGE:11942

Mcf2 mcf.2 transforming sequence ( MGI:96932)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011100. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011100_01	euxassay_011100_02	euxassay_011100_03	euxassay_011100_04

euxassay_011100_05	euxassay_011100_06	euxassay_011100_07	euxassay_011100_08	euxassay_011100_09

euxassay_011100_10	euxassay_011100_11	euxassay_011100_12	euxassay_011100_13	euxassay_011100_14

euxassay_011100_15	euxassay_011100_16	euxassay_011100_17	euxassay_011100_18	euxassay_011100_19

euxassay_011100_20	euxassay_011100_21	euxassay_011100_22	euxassay_011100_23	euxassay_011100_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11942_wholemount_strong.wlz

11942_wholemount_moderate.wlz

11942_wholemount_weak.wlz

11942_wholemount_possible.wlz

11942_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11942_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

fibula

strong

regional

strong expression: see section 01

tibia

strong

regional

strong expression: see section 01

femur

strong

regional

strong expression: see section 01 moderate expression: see section 02

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36623
Entity Detected:	Mcf2, mcf.2 transforming sequence ( MGI:96932)
Sequence:	sense strand is shown >T36623 GTTCCTTCAATGTTTGGCTAGGACACCGGAAAGGTGCTACAAAAATGAAGGATTTTGCACGTTTCAAACC CATGCAGAGACATCTTTTCTTATATGAAAAAGCTGTTATGTTTTGTAAGAGGCGTTTCGAATCTGGAGAA GGTGCTGATAGATACCCATCATACAGTTTTAAGCACTGCCTAAAAATGGAAGATGTTGGCATCACAGAAC ATGTGAAAGGTGATAACCGCAAATTTGAAATACGGTATAGTGAGAAGGAAGAAATTTACATTGTTCAGGC TCCAAATGTTGATGTGAAGATGTTATGGCTAAAAGAAATAAGAAAAATTTTGGTGCAGCAGAAGGAACTT ATGACTGCTAAAACCCAGCAGGATCAGGCACTAGACCAGGACCAACTTTTCCCCCAGCAGCAGAATGCCG AGCTATGCAAAAGCTCACCTTTTTGTGTGTGTGAGGAAACTCTTTTCAACGCCACAGAAGCAGGAGCAGA AGTGGAGCAAGCTGGTGCCCTGATAAAGGTCAGGGAGGCAGCTGTGCTAGCTCAAGCAGAGGCAAGTTCA GCAGCTTGGAATGGGATGTTACCATCTGCAGAAGGCGCTGCTGCAATTGCTGAACATTCATACAATTATT TCTCCTCTAACAATGACCATGGAGAAGATAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 66665. Forward Primer - name:066665_F_cDNA_Mcf2, sequence:GTTCCTTCAATGTTTGGCTAGG; Reverse Primer - name:066665_N_SP6_cDNA_Mcf2, sequence:CCTATCTTCTCCATGGTCATTGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11944 same embryo
	EMAGE:11946 same embryo
	EMAGE:11943 same embryo
	EMAGE:11945 same embryo
	EMAGE:11941 same embryo
	EurExpress:euxassay_011100 same experiment
	MGI:4826125 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source