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EMAGE:11944

Ly86 lymphocyte antigen 86 ( MGI:1321404)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11944 EMAGE:11944 EMAGE:11944 EMAGE:11944 EMAGE:11944
"Pseudo-wholemount" of euxassay_011090. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011090_01 euxassay_011090_02 euxassay_011090_03 euxassay_011090_04
EMAGE:11944 EMAGE:11944 EMAGE:11944 EMAGE:11944 EMAGE:11944
euxassay_011090_05 euxassay_011090_06 euxassay_011090_07 euxassay_011090_08 euxassay_011090_09
EMAGE:11944 EMAGE:11944 EMAGE:11944 EMAGE:11944 EMAGE:11944
euxassay_011090_10 euxassay_011090_11 euxassay_011090_12 euxassay_011090_13 euxassay_011090_14
EMAGE:11944 EMAGE:11944 EMAGE:11944 EMAGE:11944 EMAGE:11944
euxassay_011090_15 euxassay_011090_16 euxassay_011090_17 euxassay_011090_18 euxassay_011090_19
EMAGE:11944 EMAGE:11944 EMAGE:11944
euxassay_011090_20 euxassay_011090_21 euxassay_011090_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11944Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11944_wholemount_strong.wlz
11944_wholemount_moderate.wlz
11944_wholemount_weak.wlz
11944_wholemount_possible.wlz
11944_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11944_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
fibula
strong strong
regionalstrong expression: see section 01
tibia
strong strong
regionalstrong expression: see section 01 moderate expression: see section 02
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36606
Entity Detected:Ly86, lymphocyte antigen 86 ( MGI:1321404)
Sequence:sense strand is shown

>T36606
CCTCCTTGTGTGGATTCTGACTTCTCCGAGCAGCAGTGACCATGGCAGCGAAAATGGTTGGCCCAAGCAC
ACGGCCTGCAACAGTGGGGGCTTGGAAGTAGTCTACCAGAGCTGTGATCCCTTACAGGATTTTGGCCTTT
CCATTGACCAGTGTTCCAAGCAGATCCAATCAAATCTCAACATTAGATTTGGCATCATTCTGAGACAGGA
TATCAGAAAGCTGTTTCTGGACATAACTCTGATGGCAAAAGGCTCTTCTATTCTGAACTACTCCTATCCC
CTTTGTGAGGAGGACCAGCCCAAGTTCTCATTCTGTGGAAGAAGAAAAGGAGAACAGATATACTATGCCG
GCCCTGTCAATAACCCTGGACTTGATGTTCCACAGGGAGAATATCAGCTCTTGCTGGAACTGTACAATGA
AAACCGTGCTACTGTGGCTTGTGCCAATGCCACTGTCACCTCCTCCTGAGCATGGTCTGCAAGGAAATGC
ACAGTAAACTCAATCTCAGGGGACCCCAAGGTCCCTGGACTCACCTAGCTGCAAGAACCACTGATAACCA
AGAGAGGCTTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74382. Forward Primer - name:074382_F_cDNA_Ly86, sequence:CCTCCTTGTGTGGATTCTGACT; Reverse Primer - name:074382_N_SP6_cDNA_Ly86, sequence:AAAGCCTCTCTTGGTTATCAGTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11946 same embryo
 EMAGE:11942 same embryo
 EMAGE:11943 same embryo
 EMAGE:11945 same embryo
 EMAGE:11941 same embryo
 EurExpress:euxassay_011090 same experiment
 MGI:4826033 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS