EMAGE mouse gene expression spatial database

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EMAGE:12016

Il17rd interleukin 17 receptor D ( MGI:2159727)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011109. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011109_01	euxassay_011109_02	euxassay_011109_03	euxassay_011109_04

euxassay_011109_05	euxassay_011109_06	euxassay_011109_07	euxassay_011109_08	euxassay_011109_09

euxassay_011109_10	euxassay_011109_11	euxassay_011109_12	euxassay_011109_13	euxassay_011109_14

euxassay_011109_15	euxassay_011109_16	euxassay_011109_17	euxassay_011109_18	euxassay_011109_19

euxassay_011109_20	euxassay_011109_21	euxassay_011109_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12016_wholemount_strong.wlz

12016_wholemount_moderate.wlz

12016_wholemount_weak.wlz

12016_wholemount_possible.wlz

12016_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12016_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 10 11 15 16

telencephalon ventricular layer

strong

regional

strong expression: see section 12 13 moderate expression: see section 10 11 14 15

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15

midbrain ventricular layer

strong

regional

strong expression: see section 08 09 13 14 15

inner ear

moderate

regional

moderate expression: see section 04 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 15 16 17 18 weak expression: see section 10 11 14

maturing glomerular tuft

moderate

regional

moderate expression: see section 06 07 08 09 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37319
Entity Detected:	Il17rd, interleukin 17 receptor D ( MGI:2159727)
Sequence:	sense strand is shown >T37319 GCAGTCCTTGTCTTGTGCTTTATTCTCTCCTGCAGGTGAGCAGCTGGGGCTTGTGTCTGGTGTCTTCTTC AAGGCCAGCACCTGCCTACCTGCTGAGCGGCAGCTATCCCACGCGCCAACCTGGGGTTCTATTTTTACCT CTCTCTAGACTTGCCCTGTATTTTGTGAATCATATTGTTTAAATGGCACACACTTCACCAGAACCTCTGG CAAGGTGAGGCCAGAGGTTGCCAAGGGCCACCTCCCGTTGACTGTTGGGGACTATTTCCAAACACCTTGG AATGCCTCAGGGAGGGCCTCTGGAGAATTTCAGCCAGTAGTCTTCTTTCCTGCCTCTTGGTTCACCCTTG ATACTCACTGGGGACCCCAGATACCTCTCCCTCCTCCTCTAGGGCTGGATCTCTCAGTTAAGCAGGCATA ATGTTTGATGCAATCAAGAGCCAGACAGAAGCGATCACCCCCGACTGGATAGCTAATATTTATATCACAT GGACTCTGGCCGGAAGAGAAGGAAGAATATTGGCCCTGTGTGGCTACCACTGGATTATATTCAATGTGTT CATATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAAATCCATCTTTCTCTCAAAGAAGCATATCAAGT TTGAACAGAAGCAAAAACTTCTGTTCAAACTCAAGTGGTCATTTTAAGAGTAGCTTCGGTTTTTCTGATC TGTTTAAAAACAAAAGTGTTTTTACTGTGGAAGCTTCCATGTTATGGAGGAGGGCCTCAGGCGTGGGGAG GACTGGTGCCCTTGTTCTGTTTTATGTCCAAGTTTGTCCTTTCTGGGGCTTTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 65290. Forward Primer - name:065290_F_cDNA_2810004A10Rik, sequence:GCAGTCCTTGTCTTGTGCTTTA; Reverse Primer - name:065290_N_SP6_cDNA_2810004A10Rik, sequence:AAAAGCCCCAGAAAGGACAAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12012 same embryo
	EMAGE:12013 same embryo
	EMAGE:12015 same embryo
	EMAGE:12011 same embryo
	EMAGE:12014 same embryo
	EurExpress:euxassay_011109 same experiment
	MGI:4825570 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source