EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12096

Lrrn2 leucine rich repeat protein 2, neuronal ( MGI:106037)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008430. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008430_01	euxassay_008430_02	euxassay_008430_03	euxassay_008430_04

euxassay_008430_05	euxassay_008430_06	euxassay_008430_07	euxassay_008430_08	euxassay_008430_09

euxassay_008430_10	euxassay_008430_11	euxassay_008430_12	euxassay_008430_13	euxassay_008430_14

euxassay_008430_15	euxassay_008430_16	euxassay_008430_17	euxassay_008430_18	euxassay_008430_19

euxassay_008430_20	euxassay_008430_21	euxassay_008430_22	euxassay_008430_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12096_wholemount_strong.wlz

12096_wholemount_moderate.wlz

12096_wholemount_weak.wlz

12096_wholemount_possible.wlz

12096_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12096_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

adrenal medulla

moderate

regional

moderate expression: see section 10 11 12 19 20

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 05 moderate expression: see section 06 07 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 20 moderate expression: see section 19 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 08 09 20 moderate expression: see section 19

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07

trigeminal v nerve

strong

regional

strong expression: see section 10 11 moderate expression: see section 18 19

spinal cord

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 11 weak expression: see section 17

cervical ganglion

moderate

regional

moderate expression: see section 09 10 weak expression: see section 18

thoracic ganglion

moderate

regional

moderate expression: see section 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 09 10 19 moderate expression: see section 11 12 18

tongue

strong

spotted

strong expression: see section 13 16 17

tongue muscle

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18

stomach mesentery

moderate

spotted

moderate expression: see section 03 04 05 06 07 08 09 10 11

midgut

moderate

spotted

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21

bladder

moderate

regional

moderate expression: see section 12 13 14 15 16 17

renal cortex

weak

regional

weak expression: see section 08 09 10 11 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8395
Entity Detected:	Lrrn2, leucine rich repeat protein 2, neuronal ( MGI:106037)
Sequence:	sense strand is shown >T8395 CGCGCCGTGGAGCAGCGATCCCAGCCGGGAGGCGAGTGACGGACATAGGACAGCGCCCGCGCGGGTCGCC CAATGAGGAAATGAGTGGGCAGTCCCAGTCCCAGTCCCAGTCCAGTGCACAAAGATGAGGACCTACACCC AGGACAGCACAAAGTGATGTCCACCCACCCTGTGTAGAATGACTGCCCTGAGAGGGTGGTTCCCCGGACC CTGGCAGGGCTGCTGAACCTCCGCCTGCAAGTCGTGAAGAGCAGGACTCTCCCCTGCCTTCCACCTCCAC CATGAGGCTCCTCGTGGCTGCCCTCTTGCTATCTTGGGTGGCTGGTACCACCGCTGCAGCACCGGTGGTA CCCTGGCGTGTGCCCTGCCCTCCTCAGTGTGCCTGCCAGATCCGGCCCTGGTACACACCCCGCTCGTCCT ACCGTGAAGCCACCACTGTCGACTGCAATGACCTCTTCCTGACGGCAGTGCCCCCAAGGCTCCCTGCGGG GACACAGACCCTGCTCCTGCAGAGTAACAGCATCAGCCGGATAGACCAGACTGAGCTTGCCTACTTGGCC AACCTCACAGAGCTGGACCTGTCCCAGAACAGCTTCTCAGATGCCCGAGACTGCGATTTCCAAGCCCTGC CTCAGCTGCTGAGCCTACATCTAGAAGAGAACCGGCTGAACCGGCTGGAGGATCACAGCTTCGCTGGGCT GACCAGCCTGCAGGAGCTCTATCTCAACCATAACCAGCTCTGCCGCATCTCCCCCAGGGCCTTTGCAGGG CTGGGTAACCTGCTGAGACTGCACCTCAATTCCAACCTGCTTAGGACCATCGACAGCCGCTGGTTCGAGA TGCTGCCCAACTTGGAAATCCTCATGATTGGCGGCAACAAGGTGGATGCCATCTTGGACATGAACTTCCG GCCCCTGGCCAACCTGCGCAGCCTGGTGCTGGCAGGCATGAGCTTGCGGGAGATCTCAGACTACGCTCTG GAGGGACTGCAAAGCCTGGAGAGTCTCTCTTTCTACGACAATCAGCTGGCCCAGGTGCCCAAGCGGGCAT TGGAGCAGGTGCCTGGGCTCAAGTTCCTAGACCTGAACAAAAACCCACTGCAGCGGGTCGGACCGGGAGA CTTTGCCAACATGCTGCACCTCAAGGAACTAGGACTAAACAACATGGAGGAACTGGTCTCCATCGACAAG TTTGCTCTGGTAAATCTCCCTGAGCTGACCAAGTTGGACATCACCAACAACCCACGGCTTTCTTTCATCC ATCCTCGCGCCTTTCACCACCTGCCCCAGATGGAGACTCTCATGCTCAACAACAATGCTCTCAGTGCCTT GCACCAGCAGACAGTGGAGTCTCTGCCCAACCTACAGGAGGTGGGGCTCCACGGCAACCCCATCCGCTGT GACTGTGTCATCCGCTGGGCCAACGCCACGGGCACCCACGTCCGTTTCATCGAACCACAGTCCACCCTGT GTGCTGAGCCTCCAGACCTTCAGCGCCGCCCAGTCCGGGAGGTGCCCTTCCGGGAGATGACAGACCACTG CCTGCCCCTCATCTCTCCCCGAAGTTTCCCCTCCAGTCTGCAGATAGCCAGTGGAGAGAGCACGGTACTT CACTGTCGGGCACTGGCTGAACCAGAACCTGAGATCTACTGGGTCACCCCAGCTGGAGTTCGACTGACAC CTGCTCGCTCAGGCAGGAGGTACCGAGTGTTCCCTGAGGGTACCCTAGAGTTGCGGAGGGTGACGGCGGA AGAGGCAGGCCTATACACCTGTGTGGCCCAGAACTTGGTAGGGGCTGACACTAAAACCGTCAGTGTTGTG GTTGGCCACGCTCCCTTCCAGCCAGGCAGGGACAAAGGACCAGGACTGAAGCTCCATGTGCAGGAGACAC ACCCATATCACATCCTGCTGTTTTGGGCACCCCCACCCAATATAGTCTCCACCAACCTCACCTGGTCTAG CGCCTCCTCCCTCCGGGACCATGAAGCTCCTGCTTTGGCCCGACTGCCGCGGGGCACCCACCGCTACAAC ATTACCCGCCTCCTTCCGGCCACAGAGTACTGGGCCTGCCTGCAAGTGGCCTTTGCTGATGCCCACACCC AGTTGGCTTGTGTTTGGGCCAGGACTAAAGAGGCCTCTCCTTGCCACAGAGCCCTGGGGGACCGGCCCGG GCTCATAGCCATCCTGGCTCTTGCGGTCCTCCTGCTGGCAGCTGGGCTTGCAGCCCACCTCGGCAGAGGC CAGTCCAAGCAGGGGGTGGTGGGTGAGAAGCCTCTTCTTCCGGTCTGGGCTTTCTGGGGCTGGAGCGCCC CCTCTGTCCGGGTAGTGTCTGCACCCCTTGTCCTGCCCTGGAATCCAGGGAGGAAGCAGCCCAGATGTCA GATGGGGAGAGGGTGCCACCACCATTGTCTCAACATTGCTGAAGTTCCGCCAGGTCTCAGCAGTAGAGAA ATAACTAGGACAACTCTACACTAAACGGGACGTGATCTGGGCCAGATGCCTGCCAGGAACGTGGCGTTGA TTGATCCACGTGCTTGAGGCCTGGCAGCTTGACCAAGAAAGATGGGGTTCGGCAGCTCCTGGGGATGCCC TCACAGCCCCCAAATGGTTGCTGTTGCCTTCTGGGGTCCTCGGCTGTGATTCTGAGGAGAGTGTCCAAGG AACAGGAAGGACTCTGCCTAGAGCCTCCTACCGCCCCAGAGGCTTCTGGGCCTCAGCTTGGCTGTCCCCT GCCTGTGTCCCTAGGCCCTTGGCCCACAGGAAATGCCCCCTCCTCTTCTCTTTCTTTGTACAGTCTCAGT TGCTTGCTCCTCCCCCTCCTGGGCAAGGGCTGAAAGAGGCTTCTCCTACCTGTCTTGGGAGCCTGCCCCA AAAGCAGGAGTGTCCCCAGCTGGATCTGAAGGATATTTGGAGAGAGAGGTATCCAGGGATGCCTCATCTC ATAGCCTGGGCTCTGAAGTTGACTTTCTATAGGCAATTTTGTATCTTTGTGGAGAAATGTGTCACCTGTC CCCAACCCAATTCACTCTTTTTTCCTGTTTTTAAAAAAAATAAAAATAAACGATGCCAATAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5685881 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5685881 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12095 same embryo
	EMAGE:12097 same embryo
	EMAGE:12099 same embryo
	EMAGE:12098 same embryo
	EurExpress:euxassay_008430 same experiment
	MGI:4826005 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source