EMAGE mouse gene expression spatial database

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EMAGE:12198

Rnf2 ring finger protein 2 ( MGI:1101759)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007169. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007169_01	euxassay_007169_02	euxassay_007169_03	euxassay_007169_04

euxassay_007169_05	euxassay_007169_06	euxassay_007169_07	euxassay_007169_08	euxassay_007169_09

euxassay_007169_10	euxassay_007169_11	euxassay_007169_12	euxassay_007169_13	euxassay_007169_14

euxassay_007169_15	euxassay_007169_16	euxassay_007169_17	euxassay_007169_18	euxassay_007169_19

euxassay_007169_20	euxassay_007169_21	euxassay_007169_22	euxassay_007169_23	euxassay_007169_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12198_wholemount_strong.wlz

12198_wholemount_moderate.wlz

12198_wholemount_weak.wlz

12198_wholemount_possible.wlz

12198_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12198_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7143
Entity Detected:	Rnf2, ring finger protein 2 ( MGI:1101759)
Sequence:	sense strand is shown >T7143 GTGCGGCGGCGCCGGCGGTTGATTCTCGAGTCTCGCTCCGGCCACTTCCAGCGCACCCCCGGGCTGGGGC AGGAGCCGAAATGTCTCAGGCTGTGCAGACAAATGGAACTCAACCATTAAGCAAAACATGGGAACTCAGT TTGTATGAGTTACAACGAACACCTCAGGAGGCAATAACAGATGGCTTGGAAATTGTGGTTTCACCTAGAA GTCTACACAGTGAATTAATGTGCCCAATTTGTTTGGATATGTTAAAGAACACCATGACTACAAAGGAGTG TTTACATCGGTTTTGCGCGGATTGTATTATCACAGCCCTTAGAAGTGGCAACAAAGAGTGTCCTACCTGT CGAAAAAAACTGGTTTCTAAAAGATCACTAAGGCCAGACCCGAACTTTGATGCACTCATCAGCAAGATTT ATCCCAGTCGTGATGAGTATGAAGCGCATCAGGAAAGGGTCTTAGCAAGGATCAACAAACACAACAATCA GCAGGCTCTCAGCCACAGCATCGAGGAGGGGCTGAAGATACAGGCCATGAACAGATTACAGCGAGGCAAA AAGCAGCAGATAGAAAATGGTAGTGGAGCAGAAGATAATGGTGACAGCTCCCACTGTAGTAACGCATCCA CACACAGCAACCAGGAAGCGGGCCCGAGTAACAAACGGACCAAAACCTCTGATGACTCTGGGCTTGAACT TGATAACAACAATGCAGCAGTGGCGATTGATCCAGTCATGGACGGTGCCAGTGAGATTGAGTTAGTCTTC AGGCCCCATCCAACTCTTATGGAAAAGGACGACAGCGCACAGACGAGATACATAAAGACTTCAGGCAATG CCACTGTTGATCACTTATCCAAGTATCTGGCTGTGAGGTTAGCTTTAGAAGAACTTCGAAGCAAAGGAGA ATCAAACCAGATGAACCTGGATACAGCCAGTGAGAAGCAGTACACCATTTACATAGCCACAGCCAGTGGC CAGTTCACCGTTTTAAATGGCTCCTTTTCTTTGGAATTGGTCAGTGAGAAATACTGGAAAGTGAACAAAC CCATGGAACTTTATTATGCACCCACCAAGGAGCACAAATGAGCTTTTAAAAGTCAATTCTAAGACTGAAC TTTTTATAGCCTATTCCTTTACTATTAAAGATGTACCATTGTTACTTTCATGGACAGACCTGGGATATGT TCAGTTTTCTTTCTGAGCCAGACTTGTTTACACTATTAGATTACCGTCTCCTTAATTTAAAGACAGTACT TTGTGGAAAGGACTCTGTGATTCAGAGCTCCCACTCCCTCCATAGACGCTCTAAGTTGTTGCTTACAGAA AACGGGGTTCCTCTGGGGAGGAGACTTTGACCTAGGAGTTTTTTGTAGTTCTGTATTCTTTATTTTTTCC TTCCATTTTTTGATATTTTCAGACAGTGTCTCATTATTATAAATCCCTGGCTAGCCTGGAACTTGCTGAG TAGAACAGGCTGGCCTTGACTTTGGAGTAATGCTCCTGCCCAAGTACTGGGATTCTGCCTGGCTTAAGAT TCAGTTGGCCATCAGCCATCTTTTCCCCTTTCAAAAGTTTTTTAGCTTAAAGAATGTTAGACAATATGTA TTCTGATGTTTTCCCCTTGAAGTCTTGTATATTTATAGTTTTCTATACAAACTGTAGTATCTTCATGAAG ACCAAGGCTCAAATTACTGTGCTTAAAACAATTCTCATAGGATTATTATTTTCATGGTATTTTCTTACAT AATATCTCATTTAAAGGAGGTTCTTTATGAACTTGGTGTCCATTGTCATGCAATGTTTTGTTTTTGTCAT GTGTTTCCACTATAAGTTTAGAATTTCAGGTCATGGGGAAAAGTCGAATAAAGTTCTTTGAGAACGTGGT TCATTTACAGGTTTCACTGAAGAAAGAGAAGTTTAAGATGGTGAAAATATGTGTATTCCTCTGTACATTT GAGAGTTTACTGTCCCTTTATTATGTATGTTTGATGCTTTTCTCTATTAAAATACCAGAGATTATGGAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4021046 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4021046 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12201 same embryo
	EMAGE:12199 same embryo
	EMAGE:12202 same embryo
	EMAGE:12200 same embryo
	EMAGE:12197 same embryo
	EurExpress:euxassay_007169 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source