EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12199

Capsl calcyphosine-like ( MGI:1922818)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007148. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007148_01	euxassay_007148_02	euxassay_007148_03	euxassay_007148_04

euxassay_007148_05	euxassay_007148_06	euxassay_007148_07	euxassay_007148_08	euxassay_007148_09

euxassay_007148_10	euxassay_007148_11	euxassay_007148_12	euxassay_007148_13	euxassay_007148_14

euxassay_007148_15	euxassay_007148_16	euxassay_007148_17	euxassay_007148_18	euxassay_007148_19

euxassay_007148_20	euxassay_007148_21	euxassay_007148_22	euxassay_007148_23	euxassay_007148_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12199_wholemount_strong.wlz

12199_wholemount_moderate.wlz

12199_wholemount_weak.wlz

12199_wholemount_possible.wlz

12199_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12199_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

strong

regional

strong expression: see section 06 07 moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20

4th ventricle

strong

regional

strong expression: see section 05 06 07 moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 weak expression: see section 04

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 18 19 moderate expression: see section 20

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 12 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9968
Entity Detected:	Capsl, calcyphosine-like ( MGI:1922818)
Sequence:	sense strand is shown >T9968 AGGGAGACACAGGACCATGGGAGGAGACTAGAGTCCCCAGGGCCAGAAGTCCAGAGTTTCACCGTGAAGC AGCCTGCGGCTACAGACGAGCATTTCAGATGGCAGGGACAGCGCGCCATGACCGGGAGATGGCAATCCAA TCCAAGAAAAAGCTCTCCACAGCTACTGATCCTATTGAAAGACTTCGGTTGCAGTGCCTGGCCAGAGGTT CTGCAGGCATCAAAGGACTTGGCAGAGTGTTTCGAATTATGGATGACAATAACAACCGAACCCTTGATTT CAAAGAATTTTTGAAAGGCTTAAATGATTACGCTGTGGTCATGGAAAAAGAGGAGGCAGAAGAACTTTTC CAGAGATTCGATAGAGACGGCAGTGGAACAATAGACTTCAATGAGTTTCTTCTCACGTTAAGGCCTCCAA TGTCCAGAGCCAGAAAAGAGGTAATCATGAAAGCTTTTAGAAAGTTAGACAAAACTGGAGATGGGGTTAT AACCATTGAAGACCTTCGTGAAGTTTATAACGCTAAACATCATCCAAAGTATCAAAATGGGGAATGGACG GAGGAACAAGTCTTCAGGAAATTTCTGGATAACTTTGACTCACCCTATGATAAAGATGGATTGGTGACCC CCGAGGAATTCATGAACTACTATGCAGGTGTAAGTGCATCCATTGACACAGATGTGTACTTCATCATCAT GATGACGACGGCTTGGAAGCTCTAAAGACCTGTCCCTGGAAACCAGGCCATGCATGCAGTCACGTGACTC CAAGTGATTACGCTCTACCCCCAAACTCAGAATGCTCTTCAATTCACTGTTCATCAAACAGACTCTTCTT TGCCAACACACTGTATTTTCTAGGCTGACAAGAGGGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30297953 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30297953 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12201 same embryo
	EMAGE:12202 same embryo
	EMAGE:12198 same embryo
	EMAGE:12200 same embryo
	EMAGE:12197 same embryo
	EurExpress:euxassay_007148 same experiment
	MGI:4823619 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source