EMAGE mouse gene expression spatial database

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EMAGE:12200

Ap1s3 adaptor-related protein complex AP-1, sigma 3 ( MGI:1891304)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008243. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008243_01	euxassay_008243_02	euxassay_008243_03	euxassay_008243_04

euxassay_008243_05	euxassay_008243_06	euxassay_008243_07	euxassay_008243_08	euxassay_008243_09

euxassay_008243_10	euxassay_008243_11	euxassay_008243_12	euxassay_008243_13	euxassay_008243_14

euxassay_008243_15	euxassay_008243_16	euxassay_008243_17	euxassay_008243_18	euxassay_008243_19

euxassay_008243_20	euxassay_008243_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12200_wholemount_strong.wlz

12200_wholemount_moderate.wlz

12200_wholemount_weak.wlz

12200_wholemount_possible.wlz

12200_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12200_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

urinary system

strong

regional

strong expression: see section 10 11 12

bladder

strong

spotted

strong expression: see section 12

renal cortex

strong

spotted

strong expression: see section 07 08 09 14 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5838
Entity Detected:	Ap1s3, adaptor-related protein complex AP-1, sigma 3 ( MGI:1891304)
Sequence:	sense strand is shown >T5838 GCGGACGCGTGGGGGGAAGTAGCCATGGTCTGAGGCTAGCGCCTCACCTGTCAGCCGCCCGGGCTGCTAC AGTCTCTTCTGGCCCTCGCATCCCGGGCAATCGCTGCTGTGGGGACCCGGGACCATGATACATTTCATCT TGCTCTTCAGTCGACAAGGGAAGCTGCGGCTGCAGAAATGGTACACCACACTCCCTGACAAGGAGAGGAA GAAGATCACCCGCGACATCATCCAGACCGTCCTCTCTCGTGGGCACAGGACCAGCAGCTTCATTGACTGG AAGGAGCTGAAACTTGTTTATAAAAGGTATGCTAGTTTATATTTTTGCTGTGCAATAGAAAATCAAGACA ATGAGCTCTTGACACTAGAGATTGTTCATCGTTATGTGGAGTTGCTGGATAAATACTTTGGAAATGTCTG TGAGCTGGATATTATCTTTAATTTTGAAAAGGCATATTTTATCCTTGATGAGTTTATAATAGGTGGAGAA ATTCAGGAAACATCCAAGAAAACAGCAGTCAAGGCCATTGAAGACTCTGATATGTTACAAGAGACAATGG AAGAATACATGAACAAGCCCACGTTTTAAGTCTACAGTCTACTTGAAGATTCTGAGCGCTCCATGCTGTG AACCTACAAACAAGCAACTCCTTGCATCCAGTCTCCGCACGGCCTCTGGCACCATGCCAAAAACTACTTA AAGGGATTCTGTGTTAGCTAGCAAAACTTAAAGTTGTTAGCATATGTCCTTATGGTTGCTACATGTCTGT ATTATACTGTGTATCAATACTCACTGGGGCTCTAGGTGTTTCACATAATTGCTGCAGATTGAATAGCTCC TTGCTGTGTTTTAAGCCCTTGTGGTACTTTTAAGGTAATTTCAAATATTGGTTGCAATATGATCCTTTTT GTTGCCATTTGATCTTCTACCTGTAGACTGACAACTGCTTTGTGATAACACACAATTTTGAATGTAAAGT ATATTTTATATAATGAATGCCTTTCCTAAATGTTTAAAAGTGTACATAAATTAAGTTTATGTTAATAGCT CAGATTTCATACATGATGCTTAGATATTTTTAACATGTTCAATGCTACTATAATTTCAGGTCAGATACAA TGTATATGACTTTAATGTCTTGAATTCAATTTTTTTTTTTTTAAGAATTGGGATAAAACTTCATAAGAGA TTTTGATGTCTTAGAAGGTGCTAGATCAAGTTTTTTACTTTCAATTAAATATAAGGGACATTTCAGTAAG GTTGTGGTTTCTACAAACTATATATTTATTAAGGTTTCCAGAGATCTGGAGATGAAATGTTACAGCATCT CCTGCTTTTACCATAATCCTGAGAGGAAGAGCTATGGCAACATTTATATAGCAATAATTGTGAACATGAG ATACAAATGATATTAGATACTTTATTTTCAGATATCTCATTCCTTGTGAGCCTTTAAAACATATTTTAAT GTAAGATGAATCTTTAAGGCTTCCTTAAGTTATGTGTATTTCATTGTACTCTTCATGGGAGGTGACTCCC TCCCTCCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTCCCTCCCTCCCTCCCTCCCTC CCTCCCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTCTTTCCCAGAAA AATATTTTTGCTGACTGTAATTGCTGTGACCATGTTACCTTCACGCTTCCAAAACAGAAGTTCTGCATAG CTGGGTACTGAGTCTGCTCAGACATGAAGTAGGAAGCTGCCTGTATTGTAGCATTTCTTCGAGGTGTCTC TGGCTGCAGTTTTGTATATGTGTTCTCCATGTAAGTGTTGAGGTTTGGTGGCCGTTGTACCTGAGTGCTA GGACTTCTGTGGTTAAGACAATAGGGAGATCTCATACAGTGGACCCTTAGAGAATGTGGTTGTCATCTTT GGTTCATCTCATCAGACATACTTAAGGGAAGATGGCAAGACCAGAGAAGAACAGACAGACAGATGGACCC ATTCCTTAGAATAACCATTTATTTAAAAGCAATAATTCAGATTGCATATGAGCTAATAACTGGGCTTTTA ATAGCTGATTTATCTTCAAATTAATATAATTTTGGTGGCTTGTTCTTTCTGAACATCTTTGTTTCAGCAG TCTTCAGTTTTGTGCATTAACTTTTTTAAAAAGTTTGGTAAAGGTCTTTGTTTTTCAAGCATCTTTGTAA AACTGAACTTTCTGTAACTTCGGAGGAAAGTGTCTTTTGGGCTTCACTAAATGTACTACTTACATTCTGG ATAATTCCATAGTTGATCTTGTCTAGGTTGCACAAGAATTCTTCCTGTCAAGGTACTCATAGAAAGTGAT CATTACTTATGTGAGTAAATTCAGGGGATGTGGGGAACAGCTTACATGGCTTTTAGTGGAAAACCATTAT GTGTGTTTTCTTTATTATCATAAGATTGTAAGAAAAATTACCAAGTGAAAGGAAAAAAACAATAGTGAAT TTGTATAAAACACCCAAATAAAAGGTTAAAGATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3154405 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3154405 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12201 same embryo
	EMAGE:12199 same embryo
	EMAGE:12202 same embryo
	EMAGE:12198 same embryo
	EMAGE:12197 same embryo
	EurExpress:euxassay_008243 same experiment
	MGI:4823161 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source