EMAGE mouse gene expression spatial database

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EMAGE:12207

B4galt6 UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6 ( MGI:1928380)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008280. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008280_01	euxassay_008280_02	euxassay_008280_03	euxassay_008280_04

euxassay_008280_05	euxassay_008280_06	euxassay_008280_07	euxassay_008280_08	euxassay_008280_09

euxassay_008280_10	euxassay_008280_11	euxassay_008280_12	euxassay_008280_13	euxassay_008280_14

euxassay_008280_15	euxassay_008280_16	euxassay_008280_17	euxassay_008280_18	euxassay_008280_19

euxassay_008280_20	euxassay_008280_21	euxassay_008280_22	euxassay_008280_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12207_wholemount_strong.wlz

12207_wholemount_moderate.wlz

12207_wholemount_weak.wlz

12207_wholemount_possible.wlz

12207_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12207_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 08 09 10 18 20 moderate expression: see section 11 17 19

pancreas

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 18 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 16 17 19

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 15 16 17

stomach

strong

regional

strong expression: see section 05 06 07 08 09 10 11 moderate expression: see section 04

rectum

moderate

regional

moderate expression: see section 13

midgut

strong

regional

strong expression: see section 20 moderate expression: see section 12 13 14 15 16 17 18 19

liver

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

kidney pelvis

strong

regional

strong expression: see section 08 17 18

renal cortex

strong

regional

strong expression: see section 06 07 08 09 10 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7192
Entity Detected:	B4galt6, UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6 ( MGI:1928380)
Sequence:	sense strand is shown >T7192 GTTTCTCCTTCCCGGCTCGGTTCTTCTCCCCACAGCCTCCCCGGCCGGACCCCGCCACCTTCTTGGGGTT CTCCGTACATTCATGGAAGTTTAGGGCCTGGACCGTGGCCCGGAGTCTGGCCTGAGAGCGCAGCCCGCGC TACTGGCAGGGCAGAGGAAGATGTCTGCGCTCAAGCGGATGATGCGGGTCTCCAATCGCTCTCTGATTGC CTTCATCTTCTTCTTCTCCCTCTCCACGTCCTGTCTCTACTTCATCTATGTGGCTCCAGGCATCGCCAAC ACGTACCTCTTTATGGTACAAGCTAGAGGTATCATGCTGAGAGAGAATGTGAAAACAATTGGACACATGA TCAGGCTGTATACCAATAAAAACACTACGCTCAACGGAACAGATTATCCTGAAGGCAATAATACAAGTGA CTATCTTGTTCAAACAACAACGTATCTTCCGCAAAACTTCACGTATTTGCCACACCTCCCCTGTCCAGAG AAGCTGCCTTACATGCGGGGATTCCTCAGTGTCAATGTGAGTGAGATCAGTTTTGATGAAGTCCATCAGC TCTTCTCCAAGGACTCAGAGATTGGGCCAGGAGGCCACTGGAGGCCCAAAGACTGCAAGCCCAGATGGAA GGTGGCAGTTCTCATCCCCTTCCGTAACCGCCATGAACATCTTCCGATTTTTTTCCTGCACCTGATTCCG ATGCTCCAGAAACAGCGGCTGGAATTTGCCTTTTATGTCATCGAACAGACCGGCACACAACCATTCAATC GTGCGATGCTCTTTAATGTGGGTTTCAAAGAGGCCATGAAAGACAGAGCCTGGGACTGTGTGATCTTCCA TGATGTGGATCATCTGCCTGAAAATGATCGGAACTACTACGGATGTGGAGAGATGCCGCGGCACTTTGCA GCGAAGCTGGACAAGTACATGTACATTCTTCCCTATAAGGAGTTTTTTGGTGGGGTGAGTGGCCTGACGG TGGAGCAATTCAGGAAGATCAACGGCTTTCCCAATGCCTTCTGGGGATGGGGAGGCGAGGACGATGACCT TTGGAACAGAGTTCACTACGCCGGCTACAATGTAACCCGGCCAGAAGGGGACTTGGGAAAATACATATCT ATTCCTCATCACCATCGAGGGGAGGTACAGTTCCTAGGACGGTATAAATTACTTCGGTATTCCAAGGAGC GGCAGTACATCGATGGACTGAACAATTTATTATACACACCCAAAATACTGGTTGATAGGCTGTATACAAA TATATCTGTAAACCTCATGCCAGAATTAGCTCCAATTGAAGACTATTAAAAGAAGTCATTCTCATGGTGG GGTAGACCACAGTGCTGGATCATGAACTTGGCTTGGAGTGCCCTCACCGTGGAAGTTAGAGATTGGGGTG TATCTCAGTGTCCCGGTCTGTAAAAAGAGCCAGCAATCCTTAATGTTTACAGCGCGGGCTCCATCCGTCA CACTCTCCCACTCTCCCCCTGCTGGGAGCGTCCCCCCATGGCAAGCACTGTAGAGATAAGAACCCATTGC TTGGGACTGGAAGTGAAGAAAACTCCCTACACAGAGAAGATTTTTATACTGAAATCTATAATTTAATTCA AGAGAATGCTTTTATTTCCGTTCAAACATATTTTGTACATATGTGATGTAAATTAAATGTGTTCAAATTG TTGGAAAACAAGATGTCACAATTTTGCATGTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4037384 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4037384 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12208 same embryo
	EMAGE:12204 same embryo
	EMAGE:12203 same embryo
	EMAGE:12206 same embryo
	EMAGE:12205 same embryo
	EurExpress:euxassay_008280 same experiment
	MGI:4823381 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source