EMAGE mouse gene expression spatial database

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EMAGE:12297

Ddx11 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae) ( MGI:2443590)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010958. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010958_01	euxassay_010958_02	euxassay_010958_03	euxassay_010958_04

euxassay_010958_05	euxassay_010958_06	euxassay_010958_07	euxassay_010958_08	euxassay_010958_09

euxassay_010958_10	euxassay_010958_11	euxassay_010958_12	euxassay_010958_13	euxassay_010958_14

euxassay_010958_15	euxassay_010958_16	euxassay_010958_17	euxassay_010958_18	euxassay_010958_19

euxassay_010958_20	euxassay_010958_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12297_wholemount_strong.wlz

12297_wholemount_moderate.wlz

12297_wholemount_weak.wlz

12297_wholemount_possible.wlz

12297_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12297_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9377
Entity Detected:	Ddx11, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae) ( MGI:2443590)
Sequence:	sense strand is shown >T9377 GGGGCGTGGCCACGTGGAGGGTTTTCTGCGTGCGCGCATGCTCCTTCAGTGAAACTGCAGTTTAAATTCA GCCGCGGCGGCCAGGCTCGCTTGCCGGCCTCAGTGAGGCGGTCCTGCGGTGGCGATCGAGTTCGGTGCTG TGTGGGAGAAGCGCTGCGACCGAAGGCAGAGAGGGCAATGGCTGACGAAAACCAGGAGATTGGTGGCATC CATTTTCCTTTTCCCTTCCCGCCCTATCCTATCCAGAAAGACTTCATGGCAGAGCTCTACAAGGTTTTGG AAGGTGGCAAGATTGGGATATTTGAAAGTCCAACTGGCACGGGAAAGTCCTTAAGTCTGATTTGTGGGGC CCTTTCCTGGCTGCGTGACTTTGAAAAGAAGAAATTGCAAGCAGAGGCTCTTCTCCTTGCACCTGGATCC GGCCCTCCAAGTAGTGAGAAGAACTCGCTCCTGACTTCCTCATCTTGCCAAGAACCTACGGACACCCCGA GGCCTGCTGGAGAGCCTGACTGGGTCACCGAATTTGTGCAGAAGAAAGAAGAAAGGGACCTGGTGGAGAG GCTAAGGGAGGAGCAGAAACATGAGGAAGAGGAGACTGAGGCTCTCCTGCGCCTCAGCAGGGAGATGCTG GATGCAGGGACAGGACCAGAGCAGCTGGAGCAGCTGGAGTGTGGGGAGGAGCACCTGGTTCTGGCAGAAT ATGAGAGTGATGAGGAGAGAAGAGGCAGCAGGGTGGACGAGGCTGAGGATGACTTGGAGGAAGAACATAT AACCAAGATTTATTACTGCAGTCGGACACACTCACAGCTGGCCCAGTTTGTGCGGGAAGTACTGAAGAGC CCTTTTGGCAAGGAGACCCGGCTAGTCTCACTTGGCTCTAGGCAGACCCTTTGTGTGAATGAAGATGTGA AAAACCTGGGTTCTGTGCAACTTATGAATGACCGCTGTGTGGACATGCAAAGAAGCAAACGCGAAAAGAA CGGGACTGGGGAAGACAAGCCAAAGAGAAAGAGACAGAAGATCCAGACCTCTTGCCCCTTCTACAACCAC GAGCAGATGGAGCTTCTCCGGGATGAGATTCTGCTGGAAGTGAAGGACATGGAACAACTTGTGGCCCTTG GGAAGGAGGCCCGGGCCTGCCCCTATTACGGAAGCCGCTTTGCCATCCCTGCAGCCCAGCTAGTGGTGCT ACCCTACCCAATGTTGCTGCATGCAGCTACCCGACAGGCTGCGGGAATCCGGCTACAAGGTCAGGTGGTC ATTATTGACGAGGCTCACAACCTGATCGACACCATCACCAACATCCACAGCACAGAGGTCAATGGTTCCC AGCTCTGCCAGGCCCATTCCCAGTTGCTCCAGTACATGGAAAGATACAGGAAGCGTTTAAAGGCCAAGAA CTTGATGTACATCAAGCAGATCCTGTACTTGCTGGAGAAGTTTGTGGCTGTTTTGGGAGGTAATGTTAAG CAAAATCCTACTACACAGAGCCTGTCTCAAACAGGGTCTGAGCTGAAGAGCATCAACGACTTTCTCTTTC AGAGCCAGGTGGACAACATCAACCTCTTCAAGGTGCAGCGGTACTTGGAGAAGAGCATGCTCAGCAGGAA GCTCTTTGGCTTCACAGAATGTTTCGGAGTGGTTCTTCCATCCCTCTCAGACTCTCAGGAAAACCGAGGT CTGGCTGGCTTCCAGCAGTTCCTGAAGAGCCTGCAGTCAGGGCCTACTGAGGACTCCCCGGAGGAGGGCC AGGCTGTAGCCCTGCGGCCTGCCTCTCCACTGATGCACATCGAGGCCTTCCTGGCAGCTCTCACCACAGC CAATCAGGATGGCAGGGTTATTGTGAACCGCCAAGGCAGTGTGGGTCAGAGCAGCCTTAAATTCTTACTC TTGAACCCAGCTGTGCACTTTGCCCAGGTGGTGAAGGAATGCCGAGCAGTGGTCATTGCAGGTGGCACCA TGCAGCCGATGTCTGACTTTCGGGAGCAGCTGCTGGCATGTTCTGGAGTAGAAGCGGGGCGAGTGGTGGA GTTTTCCTGTGGTCACGTGATCCCTCCAGACAACATCTTGCCCCTTATTATATGCAGTGGGCCCTCTAAC CAGCAGCTGGAATTCACCTACCAGAGAAGAGAGCTGCCTCAGATGGTGGAGGAGACAGGCCGCATTCTCT GTAACTTGTGCAATGTGGTCCCTGGAGGTGTGGTATGCTTTCTCCCTTCTTATGAGTACCTGCGCCAGGT ACATGCTCACTGGGACAAGACTGGCCTGCTGACACGTTTGTCTGTCAGGAAAAAGATATTTCAAGAGCCC AAAAGAGCGAGCCAGGTGGAGCAGGTGCTGATGGCGTATTCCAAGTGCATTATGAGCTGCAGTCACTCAG AAGGCCATCTGACAGGGGCCTTGCTCCTCTCTGTGGTTGGAGGGAAGATGAGTGAAGGGATCAATTTCTC TGATGACCTGGGCCGGTGTGTGGTGATGGTGGGGATGCCATATCCCAACATTAAGTCTCCAGAGCTGCAA GAGAAGATGGCCTACTTGAATCAGACACTTCCTAGGACACAAGGTCAGCCCCTGCCAGGGACGGTATTGA TAGAGAATCTGTGTATGAAGGCTATAAACCAATCCATAGGCAGGGCCATTAGGCACCAGAGGGACTTTGC CAGCATTGTGCTCCTGGATCACCGGTATGCGCGTCCTTCTATTCTGGCGAAGCTGCCAGCCTGGATACGA GACCGAGTGGAGGTCAAAGCTACCTTTGGGCCTGCATTTGCTGCTGTGCGGAAGTTTCATCGAGAGAAGT CACACCCAAGTCTGGTATAAGCGTCTCATACCCTGCTTGACCTGGCCTTTGTATTTGGCCTAAGAAGATA GAAACCATATGCAGACTGCCAAGTGTACCCCAGCCTTCCAGAGGCAGTGGACTCTAGTGTTCAACTTGCA TAGAAAAGCCAGCAGAACATCCTGAAGGAACCTGGCCCGAGACACCTTATTCCCTGCTCAGGTTCAACTT TCCGCCTGGCTTCAGGAGGCTGTGCTAGCAGCAGCTTTCTGCCTGTTCTGAGGTGAGACCCACCTGCCTC TGCCCTTTCTGGAGACAGTTAGCTTTTCTTGGCCTTTGACTCCCATCTCAGGCACCGATGTCCCCGTTTC AAGCATCATTGCCTGACCCACTGCAGTATGACACACACACCATCTGTGCAGAAGAGAGTTTTGAGAAAAA ACTAGGACATACATAATATTGAAAGAGCTGACATGAATTTTGACTTTTCTGAAGGACCATCACAAAGACT TGGACCCTGGCCATAGACCTTTCTGTGTCCCACAATTTTTTCTCCTTCTACTTTTCCTCATAAACTAAGA GGCATTGATCCTGTCCATGGCTGTTCAGGACTCTGTGCTCAGAACATCTACCTGAAGACATGTCATTGGG CAAAAGTGTCTCCGTGTTTTTCATTTTTTTCTGCTGTCCCATGAAGGAGAACCCCAGTTAAGAGTCCAGC TCCTTTATATTCAACTGTGGCTGTCCCTTGTGTGAGCTTCAGCAACCTCAGGGGCAGTGCCCTTGCCATT TAGGCAATACACTGGTTGCATACTGCTTGCAAACTGAGAGGAGCCATAGAAGAGGACTTCTTTTTCACAG TTCTTGTGCCGCTTACAGCTCAAAAGAATTCCATTCTTGAATCTTCCTCAGATTGAAATGGTAAGGCTTC TCCAAGCAGTGGTGGCCCACGCCTTTAATCCCAGCACTTGGGAAGCAGAGGCAGGTGAATTTCTGAGTTC GAGGCCAGCCTGGTCTACAAAGTGAGTTCCAGGGCAGCCAAGGCTATACAGAGAAACCCTGTCTTGAAAA CAAACAAACAAACAAACAAATGGTAAGGCCTCATACCTGAGGGATAGTGTTTAGAGACAGGACTCCTGTA GGGAGGTAGGGACAGATTTTTTTGTCACTAGTGTAGGGCTTTAGCATACACTAAAGTGCAGCTAGAAGTC CCCATCATAGGAGGACCCAGAGAATGGCAGCCTCTGTAAACCTTGAAGGGTTGTCACTGGGAATCCACTC ATCTGTTACCTGTTATAGCAGAGTTCCTAGGCTTACATCCTAGGAATGTCATCCATGTGTCATGGCCGAT TAAAAGAACAACTGAACCTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6854039 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6854039 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12298 same embryo
	EMAGE:12294 same embryo
	EMAGE:12293 same embryo
	EMAGE:12295 same embryo
	EMAGE:12296 same embryo
	EurExpress:euxassay_010958 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source