EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12305

Ctrl chymotrypsin-like ( MGI:88558)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006973. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006973_01	euxassay_006973_02	euxassay_006973_03	euxassay_006973_04

euxassay_006973_05	euxassay_006973_06	euxassay_006973_07	euxassay_006973_08	euxassay_006973_09

euxassay_006973_10	euxassay_006973_11	euxassay_006973_12	euxassay_006973_13	euxassay_006973_14

euxassay_006973_15	euxassay_006973_16	euxassay_006973_17	euxassay_006973_18	euxassay_006973_19

euxassay_006973_20	euxassay_006973_21	euxassay_006973_22	euxassay_006973_23	euxassay_006973_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12305_wholemount_strong.wlz

12305_wholemount_moderate.wlz

12305_wholemount_weak.wlz

12305_wholemount_possible.wlz

12305_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12305_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10009
Entity Detected:	Ctrl, chymotrypsin-like ( MGI:88558)
Sequence:	sense strand is shown >T10009 ATTCCTTATTTGTCACAATGCTACTGCTCAGCCTAACCCTTAGCCTGGTCCTCCTTGGCTCCTCCTGGGG CTGTGGTGTTCCTGCCATCACGCCTGCACTGAGCTACAATCAGAGAATTGTCAACGGGGAGAATGCAGTG CCAGGCTCCTGGCCCTGGCAGGTGTCTCTCCAGGATAACACCGGCTTCCACTTCTGCGGTGGTTCTCTCA TCAGTCCGAACTGGGTGGTCACGGCTGCCCACTGCCAAGTCACGCCTGGACGCCACTTTGTCGTTTTGGG AGAATATGACCGATCTTCCAATGCTGAACCTGTGCAGGTCCTCTCGATCGCAAGGGCCATCACACACCCT AACTGGAACGCCAACACCATGAACAATGACCTGACTCTCCTGAAGCTTGCCTCGCCAGCCCGGTACACAG CACAAGTCTCACCAGTCTGCCTGGCTTCCACAAACGAGGCACTGCCTTCGGGGCTCACCTGTGTCACCAC TGGCTGGGGCCGAATCAGTGGTGTGGGCAATGTGACACCAGCTCGCCTGCAGCAAGTTGTTCTACCCCTG GTCACTGTGAATCAGTGTCGGCAGTACTGGGGTGCACGCATTACCGATGCCATGATATGTGCAGGTGGCT CAGGCGCCTCCTCATGTCAGGGTGACTCAGGAGGCCCTCTTGTCTGCCAGAAGGGAAACACCTGGGTGCT TATTGGGATTGTCTCCTGGGGCACTAAGAACTGCAACATACAAGCACCGGCCATGTACACTCGGGTCAGC AAGTTCAGTACCTGGATCAACCAAGTCATGGCCTACAACTAAACTGTCCACAGATCCGTTCCCCATCTCA ATCTAATAAACATACTCGTCTCAGTTTTAAAAAAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30308135 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30308135 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12308 same embryo
	EMAGE:12307 same embryo
	EMAGE:12306 same embryo
	EMAGE:12309 same embryo
	EMAGE:12310 same embryo
	EurExpress:euxassay_006973 same experiment
	MGI:4824111 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source