EMAGE mouse gene expression spatial database

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EMAGE:12339

Lpar1 lysophosphatidic acid receptor 1 ( MGI:108429)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007050. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007050_01	euxassay_007050_02	euxassay_007050_03	euxassay_007050_04

euxassay_007050_05	euxassay_007050_06	euxassay_007050_07	euxassay_007050_08	euxassay_007050_09

euxassay_007050_10	euxassay_007050_11	euxassay_007050_12	euxassay_007050_13	euxassay_007050_14

euxassay_007050_15	euxassay_007050_16	euxassay_007050_17	euxassay_007050_18	euxassay_007050_19

euxassay_007050_20	euxassay_007050_21	euxassay_007050_22	euxassay_007050_23	euxassay_007050_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12339_wholemount_strong.wlz

12339_wholemount_moderate.wlz

12339_wholemount_weak.wlz

12339_wholemount_possible.wlz

12339_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12339_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4645
Entity Detected:	Lpar1, lysophosphatidic acid receptor 1 ( MGI:108429)
Sequence:	sense strand is shown >T4645 CCACGCGTCCGCTCAGCCCGGGAGGGAAGTCGCCGCAGCCGCCCGGGCGGCCCTCCCGACCTTTGGCCAG GCTTACAGTTCCTCCTAGCATGACCGAGATCTGATCAGCCAACCCGCGCATTGCTTTTTGTGCCTGGCAC TGCAGTGCAGGGGGCCTCTTCATCGCCCCAAACTACAGCACTGTCATGGCAGCTGCCTCTACTTCCAGCC CTGTAATTTCACAGCCCCAGTTCACAGCCATGAACGAACAACAGTGCTTCTACAATGAGTCTATCGCCTT CTTTTATAACCGGAGTGGGAAATATCTAGCCACAGAATGGAACACAGTGAGCAAGCTGGTGATGGGACTG GGCATCACTGTTTGCGTGTTCATCATGTTGGCCAATCTCCTGGTCATGGTGGCAATCTACGTCAACCGCC GCTTCCATTTCCCTATTTATTACTTGATGGCCAACCTGGCTGCTGCAGACTTCTTCGCTGGATTGGCCTA CTTCTACCTGATGTTCAATACAGGACCTAATACCCGGAGACTGACTGTTAGCACGTGGCTCCTCCGGCAG GGCCTCATTGACACCAGCCTGACAGCTTCTGTGGCCAACCTGCTGGCTATTGCTATCGAGAGGCACATCA CGGTTTTCCGCATGCAGCTCCATACACGAATGAGCAACCGGCGCGTGGTGGTGGTGATTGTAGTCATCTG GACTATGGCCATTGTGATGGGTGCTATACCCAGTGTGGGCTGGAACTGCATCTGTGATATCGATCACTGT TCCAACATGGCACCCCTCTACAGTGACTCCTACTTAGTCTTCTGGGCCATTTTCAACCTGGTGACCTTTG TGGTCATGGTGGTTCTCTACGCTCACATCTTTGGCTATGTTCGCCAGAGGACTATGAGGATGTCTCGGCA TAGTTCTGGACCCAGGAGGAATCGGGACACCATGATGAGCCTTCTGAAGACTGTGGTCATTGTGCTTGGT GCCTTTATTGTCTGCTGGACTCCGGGATTGGTCTTGTTATTGCTGGATGTGTGCTGCCCGCAGTGCGATG TCCTGGCCTATGAGAAGTTCTTCCTCCTCCTGGCCGAGTTCAACTCTGCTATGAACCCCATCATCTACTC CTACCGCGACAAAGAGATGAGCGCCACCTTCAGGCAGATCCTGTGTTGCCAGCGCAACGAGAACCCTAAT GGCCCCACGGAAGGCTCTGACCGCTCTGCCTCCTCCCTCAACCACACCATTCTGGCTGGAGTTCACAGCA ACGACCACTCTGTGGTTTAGAAGGAAGCCAGCCGGCCTCTGTGGATCTGTGAACCCCACCCTACCCCCCA TTGCCAGGGCAAGGTGGGGAGCCAGAGGAGATGAGGACACTCCTGTACTTAACACTAACCAATGGCAGTA TTTGTCCCTAGACCCAAGAGACTTGAGGATGAATTTATTTGGCAGGCCCCATCTTCTCCTTTGGAAAACA GAAGGGGACCGTCTTGTGGTGGAATTGAGAAATGGACTCTGGGGTGACCGTGTAGCATTCACTAACTAGA CTTAAAAGATTTTATGTGGTTTGGCTTAAGCCAGGAAAAAAAAATCTGCTGAATTGAGTATACAATCGAG TATACACAGGCTTCCCCTTTAAAGAACAAACAATACATTGCATTTATTAATGAGTATGTTTATGCCTGAC AGCATGTTTGTGATCGAAAAGACTGCTAAACTGACATAGATGAGTTGTTTTTTTTTTTTTGTTTTTTGTT TTTTTTTACATGATGGAGGAAAAGTATAAATTAGAATGATTTTTGTGTTTGTTTAGAAAGCAAGCATGTG GTGTGTGTATTCAGTATGCCTTTCTTTAAAGATAAAAGGCCACTATTTTAAATCTTCTAGGGAATAGAAG AATCTAGTAAAAACCAGTATTCATTTAGGCTACAGGAAAAACCATATCCTAATCAATTACCTTTTAATTA AAGTAATGAAATATACATGAAAGGCAAAGTAATGTGAGCTTGTCACCCAAAGAGTGTGTGCTCTCCAAAC GCTGGAGGAGATGAAGCTGTAGCGTTGTCCCTGCATAGTGAAGATACCCACGTGCGTTCTCAGTGCCAGA CCCTCAGTGGGACTTGTTTTAAAGCCTGTGGTTTTCCAAGTTAGAAAATAATACCTACTTACTATAGAAA ACTTGAAAATTGCAGAACTGTGTGAAAAAAGAAAAAAGAAAGAAAGATTGCTGATATACGACCTGGAAGT AGCCATCTTTCCCTGCTCACCCACGTATGCCTATAGACATATATTATATACCTTTTTTTTTTTTTACATA ATCGGGTTGATATTGTAAAATGTTTTGTAACTTTTTCTTTCAGAATTGCCAGGTTTTTCTATGGCATTTT TTTTCAAAAACAGTGTTAGCGGCTATATAATATTCCATTTAATGGATGCAGCTCAGTTTATTTAACCATT CCTGTATTGTTGACTATGTTTTTAATTTTTTTCATTATTATAATATTGGAAGAATCTTGTGTATATAAAA CTTTGCCAGTATCATTGATGATTTACTTGGGCTCCATTCCCATGAAGGACCTACTTTTAAAATGTGAAAT GTCCTCATGTGTTGTCACTTTATAAAAAGGGATATTCTACCCTCAAACTGCAGGGGTGACCATGCTGACA AAAAGCTCAGAAATTACCTTTTTACAAAAGAAACACAGTGGCTACTTTAGTTGCGAATGGGTTCTTGACA AGATGTTTCCCAATAACCCAGACCCTTAACATAACTAGCAAATTTACTTTAGTAGGAACTGGTGATCCCT TTCTGGACACAAGAAAACACAAAGACATCAAGCATCAGAGTGAATCCAGCAATGCAGTACCAAGCAGCCT GGGTGGGGTGTGCTAGCAACTGGCTGCTGTGTGGTCTCCAGCAATCACCCAATGGACACCCTGTGTTCAG AGATGCCCAGTACAGTGTCCTGGAGACTAAGGTCTCTAATGTCATGCTATGAGGAACAAGGAGGAAGAAG AATAGTCCTGAAGCAGGTGTGGAGAGGTCAGGAGGACGTCTGAGAGAAGGCAGTCCTGCCAAGAGTGGCA TTCTAGGGAGGAGGAAGGCAGGGTGTGGCTTGTGTCTGGACAGTCAGTGGTAGGCATGTGACATTTGCCT GGTGGAAAAAAAATAAGTAGGCGGAGATCCAGTTAGCAGGACAAAGATTTTGCTCGAGGATTCACAAAAT GCAAAGAAATTTATACTGGAAATGAGTGAAACGAACTTGGACTTTTATTGATGCCCTTTTTATGGGGGAG GTAAGGACTATTTGAATTGAAAAGCATACTAATTGAACCTTAATAAATCATTCTCAATCAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:5009055 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5009055 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12342 same embryo
	EMAGE:12338 same embryo
	EMAGE:12341 same embryo
	EMAGE:12337 same embryo
	EMAGE:12340 same embryo
	EurExpress:euxassay_007050 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source