EMAGE mouse gene expression spatial database

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EMAGE:12753

Cntnap2 contactin associated protein-like 2 ( MGI:1914047)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011473. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011473_01	euxassay_011473_02	euxassay_011473_03	euxassay_011473_04

euxassay_011473_05	euxassay_011473_06	euxassay_011473_07	euxassay_011473_08	euxassay_011473_09

euxassay_011473_10	euxassay_011473_11	euxassay_011473_12	euxassay_011473_13	euxassay_011473_14

euxassay_011473_15	euxassay_011473_16	euxassay_011473_17	euxassay_011473_18	euxassay_011473_19

euxassay_011473_20	euxassay_011473_21	euxassay_011473_22	euxassay_011473_23	euxassay_011473_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12753_wholemount_strong.wlz

12753_wholemount_moderate.wlz

12753_wholemount_weak.wlz

12753_wholemount_possible.wlz

12753_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12753_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forebrain

strong

regional

strong expression: see section 03 04 05 06 07 08 09 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain

strong

regional

strong expression: see section 04 05 06 07 08 09 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain

strong

regional

strong expression: see section 07 08 09 11 12 13 14 15 16 17 18 19 20 21

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 08 21 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 09 10 19 20 21

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23 24

spinal cord

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19

neural retina

strong

regional

strong expression: see section 01 02 03 04 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30308
Entity Detected:	Cntnap2, contactin associated protein-like 2 ( MGI:1914047)
Sequence:	sense strand is shown >T30308 AGGGCACACCTGAGCAAGCTGAAAGAAAGAGAAACGGGAAAAATCGACCAAGAAATTCACAAATACAACA CCCCAGGCTTCACAGGCTGCCTGTCCAGAGTCCAGTTCAACCACATCGCCCCTCTCAAGGCAGCCTTGAG ACAGACAAACGCCTCAGCCCACGTGCACATTCAGGGTGAGCTGGTGGAATCTAACTGCGGGGCCTCCCCA CTGACGCTTTCCCCCATGTCTTCAGCCACTGACCCTTGGCACCTAGATCACTTGGATTCAGCCAGTGCTG ATTTCCCATATAACCCAGGACAAGGCCAAGCTATAAGAAATGGAGTCAACAGAAACTCAGCTATCATTGG AGGGGTCATCGCTGTGGTGATTTTCACCATCCTCTGCACCCTGGTCTTCCTCATCCGGTACATGTTCCGT CACAAGGGCACCTACCACACCAATGAGGCCAAGGGAGCTGAGTCAGCCGAGAGTGCAGATGCTGCCATCA TGAACAACGACCCCAACTTCACAGAGACCATTGACGAGAGCAAAAAGGAGTGGCTCATTTGAGGGGTGGC TTTTTAGCCATGGGATAGGGAGGGAGGAGTTAAGGGAGGAGGGGGAGGGACAGAGCACCCTGCGTCATAC CCCTGAGCACACGCTTACACTATCAGCACAAGCTGGGGAAGGCAAGCAACCCAATACTCTTAAGACTGAT GGCCACACAAAATATACTTTTTAATATTTCTTTATAGCTGAGTTCCCGCCCCCTTCTGTATCAAAACAAA ATAATACAAAAATGCTTTTAGAGTTTAAGCAACAGTTGAACTTTGTAGGTACTATCTGTCTTATTTTGTG TGTGTTTAGAGGTGTTCTAAAAACCCGTGGTAACAGGGCAAGTTTTCTACATTTTTAAGAGCCCTTAGAT CGTGGATATTTTTTTCTCAAGAAAAACTGATACATATCCATGCAGCCTCCGACATT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4456740), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 27650. Forward Primer - name:027650_F_IRAV77-80_J24_Cntnap2, sequence:AGGGCACACCTGAGCAAG; Reverse Primer - name:027650_R_SP6_IRAV77-80_J24_Cntnap2, sequence:GAATGTCGGAGGCTGCAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12751 same embryo
	EMAGE:12748 same embryo
	EMAGE:12750 same embryo
	EMAGE:12749 same embryo
	EMAGE:12752 same embryo
	EurExpress:euxassay_011473 same experiment
	MGI:4823965 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source