EMAGE mouse gene expression spatial database

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EMAGE:12919

Fancc Fanconi anemia, complementation group C ( MGI:95480)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013690. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013690_01	euxassay_013690_02	euxassay_013690_03	euxassay_013690_04

euxassay_013690_05	euxassay_013690_06	euxassay_013690_07	euxassay_013690_08	euxassay_013690_09

euxassay_013690_10	euxassay_013690_11	euxassay_013690_12	euxassay_013690_13	euxassay_013690_14

euxassay_013690_15	euxassay_013690_16	euxassay_013690_17	euxassay_013690_18	euxassay_013690_19

euxassay_013690_20	euxassay_013690_21	euxassay_013690_22	euxassay_013690_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12919_wholemount_strong.wlz

12919_wholemount_moderate.wlz

12919_wholemount_weak.wlz

12919_wholemount_possible.wlz

12919_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12919_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 03 05 06 07 21 22 23 weak expression: see section 02 04 08 18 19 20

clavicle

moderate

regional

moderate expression: see section 07 08 17 weak expression: see section 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31673
Entity Detected:	Fancc, Fanconi anemia, complementation group C ( MGI:95480)
Sequence:	sense strand is shown >T31673 CCCACGTCCTCTGTGGTCAGCCTCTGGTTTCGGCATCTCCCCAGTCTTGAAAAAGCAACGCTGCATCTTT TTGAAAAGCTTTTCTCCAGCAAGATAATTTGCCTGAGAAGGATGGAGTGCTGTATAAGAGAGTCATTCCT GCCTCAAGCAGCCTGCCAACCTGCCATCTTCAGAATTGTTGATGAAATGTTCAGGTTTGTGCTGCTGGAA ACTGACGGAGCCCCAGAAGTACTAGCTGCTCTTCAGGTTTTCACATCGTGCTTGGTAGAAGCTCTGAAAA AAGAAAACAAGCAGCTGACGTTTGCCCTCAGGACCTACTTTCCTTACGGTGCTCCATGTCTTGCTGCAGC GCTGTCCCAGCACCCTGAAGCAATCCCACAGGGACACCGGCTCCAGCCTCTGCTGCACATTTCCCAACTC CTCAGAGAAGCAGTTGAAGACTGTACTCGTGGGTCTCCGCGAAATCCCTTTGAGAGCTGGTTTTTGTTTG TTCACTTTGGAGGATGGGTTGACCTGGCTGTGGCAGAGTTACTGCTGAGGGAGGAAGCTGAGCCTCCTGC TGGCCTGCTGTGGCTCTTGGTGTTCTATTACAGCCCACAGGATGGGAGTCAGCAGAGAGAGCAGAGCATG GTGGAGCTGAAGGTATTAATCAACCGTCTCCTGATGCTGCTCAGAAGCGGCCCCCTCTCAGCTACTGATC TGCAGGAAGCAGCTGAGAGTCCCAGTGGAGACCCCAGACCACCTGTATGTGGACAGCTGGTCAGACGCCT TCTTCTTAGTCTCTTGCTCTGGACCCCAGAAGGCCATGCAATTGTCTGGGAAGCTGTCACCCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5375091), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 33169. Forward Primer - name:033169_F_IRAV81-84_B12_Fancc, sequence:CCCACGTCCTCTGTGGTC; Reverse Primer - name:033169_R_SP6_IRAV81-84_B12_Fancc, sequence:ATGGGTGACAGCTTCCCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12922 same embryo
	EMAGE:12921 same embryo
	EMAGE:12924 same embryo
	EMAGE:12920 same embryo
	EMAGE:12923 same embryo
	EurExpress:euxassay_013690 same experiment
	MGI:4824773 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source