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EMAGE:12924

Arfgap2 ADP-ribosylation factor GTPase activating protein 2 ( MGI:1924288)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12924 EMAGE:12924 EMAGE:12924 EMAGE:12924 EMAGE:12924
"Pseudo-wholemount" of euxassay_013675. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013675_01 euxassay_013675_02 euxassay_013675_03 euxassay_013675_04
EMAGE:12924 EMAGE:12924 EMAGE:12924 EMAGE:12924 EMAGE:12924
euxassay_013675_05 euxassay_013675_06 euxassay_013675_07 euxassay_013675_08 euxassay_013675_09
EMAGE:12924 EMAGE:12924 EMAGE:12924 EMAGE:12924 EMAGE:12924
euxassay_013675_10 euxassay_013675_11 euxassay_013675_12 euxassay_013675_13 euxassay_013675_14
EMAGE:12924 EMAGE:12924 EMAGE:12924 EMAGE:12924 EMAGE:12924
euxassay_013675_15 euxassay_013675_16 euxassay_013675_17 euxassay_013675_18 euxassay_013675_19
EMAGE:12924 EMAGE:12924 EMAGE:12924 EMAGE:12924
euxassay_013675_20 euxassay_013675_21 euxassay_013675_22 euxassay_013675_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12924Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12924_wholemount_strong.wlz
12924_wholemount_moderate.wlz
12924_wholemount_weak.wlz
12924_wholemount_possible.wlz
12924_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12924_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31662
Entity Detected:Arfgap2, ADP-ribosylation factor GTPase activating protein 2 ( MGI:1924288)
Sequence:sense strand is shown

>T31662
CGGTTGTGTCTGGGAAGGAGAGAAAATGGCGGCGAGCCCGAGCAAGACCGAAATCCAGACTATTTTTAAG
AGACTTCGCGCAATTCCCACCAACAAGGCCTGTTTCGATTGTGGCGCCAAGAGTCCTAGTTGGGCCAGCA
TCACTTACGGTGTATTTTTGTGTATTGACTGCTCCGGAGTGCATCGCTCCTTGGGTGTCCATCTCAGCTT
TATCAGGTCCACAGAGTTGGATTCGAACTGGAGCTGGTTGCAGCTGAGGTGTATGCAGGTCGGCGGGAAT
GCCAATGCGACTGCTTTTTTCCGCCAACACGGATGTATGGCCAATGATGCTAACACCAAATATACGAGCC
GAGCTGCCCAGATGTACCGAGAGAAGATCCGGCAGTTAGGGAGTGCAGCTCTGACTAGGCATGGTACTGA
TCTTTGGATAGACAGTATGAACAGTGCTCCAAGTCACTCTCCAGAGAAGAAAGACTCTGATTTCTTCACA
GAACATACTCAGGCCCCTGCTTGGGATACAGCAGCCACTGATCCTTCAGGGACTCAGCAACCAGCCCTAC
CTTCAGAGAGCAGTAGCCTGGCACAACCTGAACAAGGCCCTAACACTGATCTGCTCGGAACCTCACCCCA
AGCCTCTCTGGAACTGAAAAGCTCCATTATTGGCAAGAAGAAGCCAGCAGCCGCTAAGAAAGGGCTGGGT
GCCAAGAAAGGCCTGGGAGCTCAGAAGGTGAGCAACCAGAGCTTTACTGAGATTGAGCGGCAGGCCCAGG
TGGCAGAGAAGCTCCGAGAGCAGCAGGCAGCTGATGCCAAGAAGCAGGCAGAAGAGTCCATGGTTGCCTC
CATGCGTCTGGCCTACCAGGAGCTCCAGATTGATCGCAAGAAGGAGGAGAAAAAGCTCCAGAATCTTGAA
GGAAAGAAGCGAGAGCAGGCTGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3487811), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 34942. Forward Primer - name:034942_F_IRAV08-11_N22_Zfp289, sequence:CGGTTGTGTCTGGGAAGG; Reverse Primer - name:034942_R_SP6_IRAV08-11_N22_Zfp289, sequence:TTCAGCCTGCTCTCGCTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12922 same embryo
 EMAGE:12921 same embryo
 EMAGE:12919 same embryo
 EMAGE:12920 same embryo
 EMAGE:12923 same embryo
 EurExpress:euxassay_013675 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS