EMAGE mouse gene expression spatial database

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EMAGE:13029

Adrb2 adrenergic receptor, beta 2 ( MGI:87938)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013681. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013681_01	euxassay_013681_02	euxassay_013681_03	euxassay_013681_04

euxassay_013681_05	euxassay_013681_06	euxassay_013681_07	euxassay_013681_08	euxassay_013681_09

euxassay_013681_10	euxassay_013681_11	euxassay_013681_12	euxassay_013681_13	euxassay_013681_14

euxassay_013681_15	euxassay_013681_16	euxassay_013681_17	euxassay_013681_18	euxassay_013681_19

euxassay_013681_20	euxassay_013681_21	euxassay_013681_22	euxassay_013681_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13029_wholemount_strong.wlz

13029_wholemount_moderate.wlz

13029_wholemount_weak.wlz

13029_wholemount_possible.wlz

13029_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13029_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

naris

moderate

regional

moderate expression: see section 12 16 weak expression: see section 13 14 15

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8252
Entity Detected:	Adrb2, adrenergic receptor, beta 2 ( MGI:87938)
Sequence:	sense strand is shown >T8252 CAGGCATTCAGGCTGCGGCTGCAGGCACCGCGAGCCCGGAGCACCCACGAGCTGAGTGTGCAGGACGCAC CCCAGCACAGCCACCTACGGCCGCTGAATGAAGCTTCCAGGAGTCCGCCCCCGGCCGGCTGCGCCCCGTC GGAGGTGCACCCGCTGAGAGCGCCTGGGCACCGAAAGCCGGTGCGCTCACCTGCTAACCTGCCAGCCATG GGGCCACACGGGAACGACAGCGACTTCTTGCTGGCACCCAACGGAAGCCGAGCGCCAGACCACGACGTCA CTCAGGAACGGGACGAAGCGTGGGTTGTGGGCATGGCCATCCTCATGTCGGTTATCGTCCTGGCCATCGT GTTTGGCAACGTGCTGGTCATCACAGCCATTGCCAAGTTCGAGCGACTACAAACCGTCACCAACTACTTC ATAATCTCCTTGGCGTGTGCTGATCTAGTCATGGGCCTAGCGGTGGTGCCGTTTGGGGCCAGTCACATCC TTATGAAAATGTGGAATTTTGGCAACTTCTGGTGCGAGTTCTGGACTTCCATTGATGTGTTGTGCGTCAC AGCCAGCATCGAGACCCTGTGCGTGATTGCAGTGGATCGCTATGTTGCTATCACATCGCCCTTCAAGTAC CAGAGCCTGCTGACCAAGAATAAGGCCCGAGTGGTCATCCTGATGGTATGGATTGTATCTGGCCTTACCT CCTTTTTGCCTATCCAGATGCACTGGTACCGTGCCACCCACAAGAAAGCTATCGATTGTTACACCGAGGA GACTTGCTGTGACTTCTTCACGAACCAGGCCTACGCCATCGCGTCCTCGATTGTGTCTTTCTACGTGCCC CTGGTGGTGATGGTCTTTGTCTATTCCCGGGTCTTCCAGGTGGCCAAAAGGCAGCTGCAGAAGATAGACA AATCTGAAGGAAGATTCCACGCCCAAAACCTCAGCCAGGTGGAGCAGGATGGGCGGAGCGGCCACGGACT CCGAAGGTCCTCCAAGTTCTGCTTGAAAGAGCACAAAGCCCTCAAGACTTTAGGCATCATCATGGGCACA TTCACCCTCTGCTGGCTGCCCTTCTTCATTGTCAATATCGTGCACGTTATCAGGGACAACCTCATCCCTA AGGAAGTTTACATCCTCCTTAACTGGTTGGGCTACGTCAACTCTGCCTTCAATCCTCTTATCTACTGTCG GAGTCCAGATTTCAGGATTGCCTTTCAAGAGCTTCTGTGCCTTCGCAGGTCTTCTTCGAAAACCTATGGG AACGGCTACTCTAGCAATAGCAACGGCAGAACGGACTACACAGGGGAGCCAAACACTTGTCAGCTGGGGC AGGAGAGAGAACAGGAACTGCTGTGTGAGGATCCCCCAGGCATGGAAGGCTTTGTGAACTGTCAAGGTAC TGTGCCTAGCCTTAGCGTTGACTCCCAAGGAAGGAACTGTAGTACAAATGACTCGCCACTGTAATACAGG CTTTCTACTCTCTAAGACCCCTCCTTGACAGGACACTAACCAGACTATTTAACTTGAGTGTAATAACTTT AGAATAAAATTGTATAGAGATTTGCAGAAGGGGAGGCACATCCTTCTGCCTTTTTTTAAATTTTATTTTA TTTTTTAAGCTGCAAACAAGAGAGAGAAACTGTATTTGAGTGCTTATTGTTTTCTTGTATAGTTCAGTTC CTCTTTGTATGGAACTTAAAGGTTTCTGTCTGAAGAGTGTTGGTTCTGGAGGACTGAGTCTGTCTGTCTG TCTGTCTGGATGATGTTTTCATGTATCTACCTCACTGGTCAAGTATTAAGAATGATATATATTGCTGCTG GAAATCCATATCTAAAGGAGAGAGTTTTCTTCCTGTACCCTTGGACTTGAAATATCCTGTGTCTTGGACC TTTCTGCTGTGACAATGGGCCCTTTCTCTCTCACTCCACTTATTTACTCAAATGGAGTGTTGCAGGCAGG GATTTGAGGGACAACATCAGTTGTTTTGTTTTTGTTTTTGTTTTTGTTTTTTTGTTTGTTTGGTTCGTTT TTGGTTTGTTGTTGTTTTTTGGGGTTTTGTTTTTGTTTTTGTTTTTTTGGGTTTTTTTTTTTTTTTTGCT GAGAAAAGTCTAAAGTTTACAGTAAATAAATTGTTTAACCATGAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:1349283 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1349283 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13027 same embryo
	EMAGE:13028 same embryo
	EMAGE:13026 same embryo
	EurExpress:euxassay_013681 same experiment
	MGI:4823015 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source