EMAGE mouse gene expression spatial database

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EMAGE:13030

Abcc5 ATP-binding cassette, sub-family C (CFTR/MRP), member 5 ( MGI:1351644)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013680. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013680_01	euxassay_013680_02	euxassay_013680_03	euxassay_013680_04

euxassay_013680_05	euxassay_013680_06	euxassay_013680_07	euxassay_013680_08	euxassay_013680_09

euxassay_013680_10	euxassay_013680_11	euxassay_013680_12	euxassay_013680_13	euxassay_013680_14

euxassay_013680_15	euxassay_013680_16	euxassay_013680_17	euxassay_013680_18	euxassay_013680_19

euxassay_013680_20	euxassay_013680_21	euxassay_013680_22	euxassay_013680_23	euxassay_013680_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13030_wholemount_strong.wlz

13030_wholemount_moderate.wlz

13030_wholemount_weak.wlz

13030_wholemount_possible.wlz

13030_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13030_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forebrain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 09 20 21

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 18 19 20 21 22 23 24

vagus x ganglion

moderate

regional

moderate expression: see section 10 19

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 09 19 20 21

trigeminal v nerve

moderate

regional

moderate expression: see section 10 18

spinal cord

moderate

homogeneous

moderate expression: see section 11 12 13 14 15 16 17 18 19

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 13 16 17 18 19 20 21

neural retina

moderate

regional

moderate expression: see section 01 02 03 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9289
Entity Detected:	Abcc5, ATP-binding cassette, sub-family C (CFTR/MRP), member 5 ( MGI:1351644)
Sequence:	sense strand is shown >T9289 CGTTGTGGGGGACTATGCCGCTATAAAGGCTTGTTTTGCTCAGGGTCCGATGTTCGCGAGCGTGCCCGGG CTGTTTGCTCTCGTGTGGGGAGGCTGGGGTGCAGAATTTCTGAAGTGAAAGGAGGAGTCTGCACCCTGGT CCTCTGTGTGGTGAAGATGAAAGATATTGACATGGGAAAAGAATATATCATCCCCAGCCCTGGGTACAGA AGTGACAGGGACAGAAGCGCTGTACCAGGGCAACACAGAGACCCCGAGGAACCCAGGTTCCGGAGAACAA GATCGTTGGAATGCCAAGATGCTCTCGAAACAGCAGCCCGAGTTGAGGGGCTTTCCCTGGATATCTCTGT GCATTCTCATCTCCAAATTCTGGACGAGGAGCATTCTAAGGGAAAATACCACCATGGTTTAAGTGTCCTG AAGCCCTTCCGGACCACTACCAAGCACCAGCACCCAGTGGACAATGCTGGACTTTTCTCCTACATGACCT TTTCATGGCTCTCTCCTCTGGCCCGAGTGGTTCACAAGAAGGGGGAGCTGTTAATGGAGGATGTGTGGCC TTTGTCCAAGTATGAGTCTTCTGATGTGAACAGCAGAAGACTAGAGAGACTGTGGCAAGAAGAGCTGAAT GAAGTTGGGCCAGACGCTGCCTCCCTGCGAAGGGTTGTGTGGATCTTTTGCCGCACCAGGCTCATCCTGT CCATCGTGTGCCTGATGATCACGCAGTTGGCTGGCTTCAGTGGACCAGCCTTCGTGGTGAAGCACCTCTT GGAGTACACCCAGGCAACAGAGTCTAACCTGCAGTACAGCTTGCTGTTAGTGTTAGGCCTACTCCTGACA GAAGTTGTACGCTCCTGGTCACTTGCACTGACTTGGGCATTGAATTATCGAACTGGTGTCCGGTTGCGGG GGGCTATCCTGACTATGGCATTCAAGAAGATCCTGAAGTTAAAGAACATTAAAGAAAAGTCCCTAGGGGA GCTCATCAATATCTGCTCCAATGATGGGCAAAGGATGTTTGAGGCAGCCGCTGTGGGCAGCTTGCTGGCT GGAGGACCTGTTGTTGCCATCTTGGGCATGATTTATAATGTAATCATCCTAGGACCCACGGGCTTCCTGG GATCAGCGGTTTTTATCCTCTTTTATCCAGCAATGATGTTCGTGTCACGGCTAACTGCATATTTCAGGAG AAAGTGTGTAGCTGCCACAGATGACCGTGTCCAGAAGATGAATGAAGTTCTTACCTACATTAAATTCATT AAAATGTATGCCTGGGTCAAAGCGTTTTCTCAGTGTGTGCAAAAAATCCGAGAGGAGGAACGTCGGATAT TGGAGAAAGCCGGGTACTTTCAGAGCATCACTGTTGGAGTGGCTCCTATTGTGGTAGTGATCGCCAGTGT GGTGACGTTCTCCGTTCACATGACCCTGGGCTTCCATCTGACTGCGGCACAGGCCTTCACAGTGGTGACT GTCTTCAATTCCATGACTTTTGCTTTAAAAGTAACACCATTCTCAGTGAAGTCCCTCTCTGAAGCATCAG TGGCTGTTGACAGATTTAAGAGTTTGTTTCTAATGGAAGAGGTTCACATGATAAAGAACAAACCGGCCAG TCCTCACATCAAGATAGAGATGAAAAATGCCACCTTGGCATGGGACTCCTCCCACTCCAGTATACAGAAC TCGCCCAAGCTGACCCCCAAAATGAAAAAAGACAAGAGGGCTACCAGGGGCAAGAAAGAGAAGTCGAGGC AGCTGCAACACACTGAGCACCAGGCGGTGCTGGCAGAACAGAAAGGACACCTCCTCCTGGACAGTGACGA GCGGCCCAGCCCGGAAGAGGAAGAAGGCAAGCAGATCCACACAGGGAGCCTGCGCCTGCAGAGGACACTG TACAACATTGACTTAGAAATTGAAGAGGGCAAACTGGTTGGAATCTGCGGCAGTGTGGGAAGTGGAAAAA CCTCTCTCGTTTCAGCCATTTTAGGCCAGATGACGCTTTTGGAGGGCAGCATTGCCGTCAGTGGGACCTT TGCTTATGTGGCCCAACAGGCCTGGATTCTCAATGCCACTCTGAGAGACAACATTCTCTTTGGGAAGGAA TTTGATGAAGAGAGATACAACTCAGTGCTGAATAGCTGCTGCCTGCGGCCTGACTTGGCCATTCTCCCCA ACAGCGACCTGACTGAGATTGGAGAGCGAGGAGCCAACCTGAGTGGTGGACAGCGCCAGAGAATCAGCCT TGCTAGAGCCTTGTACAGTGATAGAAGCATCTACATCCTGGATGACCCCCTCAGTGCCTTAGATGCCCAT GTGGGCAACCACATCTTCAACAGTGCTATCCGGAAGCGCCTCAAGTCTAAGACGGTTCTGTTTGTTACAC ACCAGTTACAGTATCTGGTCGATTGTGATGAGGTGATCTTCATGAAGGAAGGCTGTATCACAGAGAGAGG TACCCATGAGGAGCTGATGAACTTAAATGGGGATTACGCTACGATTTTTAATAACCTGTTGCTGGGAGAG ACACCCCCAGTTGAGATTAATTCGAAAAAGGAAGCTACTGGTTCACAAAAATCACAAGACAAGGGCCCTA AGCCAGGGTCAGTGAAGAAGGAGAAGGCGGTGAAGTCGGAGGAAGGGCAGCTTGTGCAGGTGGAGGAGAA AGGGCAAGGTTCTGTGCCTTGGTCAGTCTACTGGGTCTACATCCAGGCTGCAGGGGGCCCCTTGGCTTTC CTGGTCATCATGGTCCTCTTCATGCTGAATGTGGGCAGCACTGCCTTCAGCACCTGGTGGCTTAGCTACT GGATCAAGCAAGGAAGCGGGAACAGCACAGTGTATCAAGGGAACAGAAGCTTCGTGAGTGACAGCATGAA GGACAACCCCTTCATGCAGTACTACGCCAGCATCTACGCCCTCTCCATGGCAGTCATGCTGATCTTGAAA GCCATTCGAGGAGTTGTCTTCGTCAAGGGCACACTGAGAGCCTCCTCCCGGCTCCATGATGAGCTATTCC GAAGGATCCTTAGGAGCCCCATGAAGTTTTTTGATACTACCCCAACAGGAAGGATTCTCAACAGGTTTTC CAAAGACATGGATGAAGTGGATGTGCGGCTGCCGTTCCAGGCTGAGATGTTTATTCAGAATGTAATCCTG GTGTTCTTCTGTGTTGGAATGATTGCTGGAGTCTTCCCATGGTTCCTCGTGGCGGTGGGGCCTCTCCTCA TCCTCTTCTCACTTCTCCACATTGTCTCCAGGGTCCTGATTCGTGAGCTAAAGCGGTTGGACAATATCAC GCAGTCTCCTTTCCTCTCCCACATCACGTCTAGCATTCAGGGCCTGGCCACCATCCATGCCTACAACAAA AGGCAGGAGTTTTTACACAGGTATCAGGAGCTCCTGGATGACAACCAGGCTCCCTTTTTCCTGTTCACCT GTGCAATGAGGTGGCTGGCAGTGCGGCTGGACCTCATCAGCATTGCCCTGATTACCACCACTGGCCTGAT GATTGTTCTCATGCATGGACAGATCCCTTCAGCCTATGCGGGGCTTGCCATCTCCTACGCTGTGCAGTTA ACTGGACTATTCCAGTTCACCGTCAGACTGGCATCGGAGACAGAAGCACGGTTCACTTCCGTGGAGAGGA TCAACCACTATATCAAGACTCTCTCTTTGGAAGCACCTGCCAGAATCAAGAACAAGGCTCCTCCCCATGA CTGGCCCCAGGAGGGAGAAGTAACCTTTGAGAATGCAGAAATGAGATACCGGGAAAATCTCCCTCTGGTC CTTAAGAAAGTGTCCTTCACCATCAAGCCCAAGGAAAAGATAGGCATTGTGGGACGAACAGGGTCAGGGA AGTCCTCTTTGGGGATGGCCCTCTTCCGTCTGGTGGAGCTATCTGGAGGCTGCATCAAGATTGATGGAAT AAGAATCAGTGACATCGGCCTGGCCGACCTCCGAAGCAAACTGGCCATCATTCCTCAGGAGCCAGTGCTG TTCAGTGGCACTGTCAGATCAAACCTGGACCCTTTCAACCAGTACACGGAAGACCAGATCTGGGATGCTC TAGAGAGAACGCACATGAAGGAATGTATTGCCCAGCTACCTCTGAAACTTGAGTCTGAAGTAATGGAGAA CGGGGACAACTTCTCTGTTGGGGAACGGCAGCTGTTGTGCATAGCAAGAGCCCTGCTGCGTCACTGTAAG ATTCTGATTTTAGATGAAGCTACAGCCGCTATGGACACAGAGACAGACTTACTGATCCAGGAGACCATCC GGGAAGCATTTGCGGACTGCACCATGCTGACCATTGCCCATCGCCTGCACACAGTTCTGGGCTCTGACAG GATCATGGTGCTGGCCCAGGGACAGGTGGTGGAGTTTGACACCCCATCGGTCCTTCTGTCTAATGACAGT TCAAGATTCTATGCCATGTTTGCTGCTGCAGAGAACAAGGTGGCTGTCAAGGGCTGAGTCCTCCCGTCCT CGAAGTCTCTTCCTCAGAGCATTGCCATTCTCTTCCTGGTTTGGGCCCCTCATCCTGTCCTGCTGAAACT TCGCCTTTCCCAGTTTTATCTCTCACACAACCATTCAGGATTAGCTGTGTGTTTCACTTTTAAGGAAAGT CATATTTTGATTATTGTATTTATTCCCTATTCACTTAAATGAAATTTAGTTTTTGTTCTTAATTGCACTC TTAAGAGGTTCAGGGAACCATTACTATAAATGTTATCAGTGGCCTATAATGAAGCTTTATATGTGTAGCT ATGTCTATATATAATTCTGTACATAGCCTATATTTACAGTAAAATGTAAGCGGTTTATTTTCTATTAAAG TGAGCACTGTGCTAACAGCAGTGCACACTCATTTCTATCGTTTTTGTACAGCTGACTGCACTACAGGTCA TTTTGCTCTTAGCTGTAGCAAAGGCAGATTCCGTCTCTAGCTGGTCGTTTCACGGTGCTGGGCTCTCTAG GCGTCCAGGGGAGACACGCGGCAGCAGTGGGCCCTCCATGGCCTGCCCCTTGGGCTCCCCAGTGGGAACC CAGTATAGTGGGCTGCAGGAGGCCTTGCAGAGCACCGTGAATTCTCAGGGCTCCTGCTTTCCTGTCTTGG TGTCAGCTGCTGTTTATGTTAGAGCAGTGGGCAAAGCCAGCCCCCACCCCACCCCCATTCCCTCTCAGGA ATGAGAACCTCTGCATTCCTCAGAGGGAGAGCTTCTTTCTGCCTTCCTTCTTCTGCTGCCATTTCTAAGC AAGATCCAGTCTGCTCCCAGTGTTCCATGCTTCAGGTTCCTGAGGCTGGTCGCCACAGAGTTGTCGAGTT CCCTGGCCAGCTGGTTCCCAGCCCCAGGGTCCACTGCTGCTGTTGTAGGTGGCATTTTCATTTGCCTGAC CCCAAGGCTCCAGAGCTCAGCAACAGGGCTCAGGATGGTGGGGTCCGTTCTTCCTCACTTTAGTCTCCTC TGCAAAGTCTGCACCCACCCCTCAGCAGCTCTTGCTAATCAGTGTCTCACACTGGTGTAGAAGTTTTTTG TACTGTAAAGAGACCTACCTCAGGTTGCTGATTGCTGTGTGGTTTGGTGCGCTCTTGCAGACCCCCTTGT GCTCTGGGGCTCGTAGCTTGGGTGGGTGTGGTTGCCACTGTCACCAGTCGAGTGGTCAGCGTCGCATGTC GTGACCAACTAGACATTCTGTCGCCTTAGCATGTTTGCTAAATACCTTATAAAAGCAAAAATATGAAAAA GTGAATAAAATTATTTTGGATTTGTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6839816 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6839816 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13032 same embryo
	EMAGE:13033 same embryo
	EMAGE:13031 same embryo
	EurExpress:euxassay_013680 same experiment
	MGI:4822864 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source