EMAGE mouse gene expression spatial database

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EMAGE:13147

Tiam2 T-cell lymphoma invasion and metastasis 2 ( MGI:1344338)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017276. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017276_01	euxassay_017276_02	euxassay_017276_03	euxassay_017276_04

euxassay_017276_05	euxassay_017276_06	euxassay_017276_07	euxassay_017276_08	euxassay_017276_09

euxassay_017276_10	euxassay_017276_11	euxassay_017276_12	euxassay_017276_13	euxassay_017276_14

euxassay_017276_15	euxassay_017276_16	euxassay_017276_17	euxassay_017276_18	euxassay_017276_19

euxassay_017276_20	euxassay_017276_21	euxassay_017276_22	euxassay_017276_23	euxassay_017276_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13147_wholemount_strong.wlz

13147_wholemount_moderate.wlz

13147_wholemount_weak.wlz

13147_wholemount_possible.wlz

13147_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13147_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9364
Entity Detected:	Tiam2, T-cell lymphoma invasion and metastasis 2 ( MGI:1344338)
Sequence:	sense strand is shown >T9364 GAGCCGGGCTCGACCGGTCCTCTGTGTTGAAACTTGGACCGAGGATCACTTCATGGATTTGCCTCACGTA CCTATGTTTGTTAATTTCCTGTGTCGTTCCCAAGATCACGTAAAACCCTCTGAGCATTATTGACCTCCCT CCCAGCAAAAGCCAGGCTGCAGTGTAATTTAGACCATGGCTCATATTGATACAACTGTTCTCAATTTTGG TTAAAATGGGGAACTCCGAGAGTCAATATACCTTCCAAGGATCCAAGAATCATAGTAATACTGTCACTGG TGCTAAGCAAAAGCCTTGCTCTCTGAAAATACGCAGCGTTCATGCAAAAGACGAGAAGTCCTTACATGGT TGGACTCATGGGAGCAGCGGTGCAGGCTACAAGTCCAGGTCCCTAGCCCGAAGCTGCCTTTCTCACTTTA AGAATCACCAGCCTTACGCCACCAGACTCAGTGGACCCACATGTAAAGTCTCAAAGGGCACCACCTACTC TAAGCACAGAGCAAATACCCCGGGAAATGATTTCCAGGGCAACAGTGGTGCTTTCTTACCTGAGAATGGC TTCCACTATGTTGACCGCGAGTCAGAGGAAAGCCATATCACCTCCAATGGGCACCTTCTCACCTGCTATG GGAGAAAGGAAAGCCTCGCCTCCACTCCTCCAGGCGAGGACCACAGGAGCCCCAGGGTGCTCATCAAGAC CCTGGGAAAGCTAGACGGGTGTTTAAGAGTCGAGTTCCACAACGGTGGCAACCCCCACAAGGGGACCTCC GAGGACCCCAGTGGACCTGTACGGCTGCTGAGATACTCCCCTACCTTAGCATCGGAAACCTGCCCGGTGC GGGAAACCAGGCGGCATTCCGCTGCAGGCTCCCCATCCAGCCAGCGGCCCTCTCCCACTGACTCTCGCCT GCGCTCCAGCAAAGGCAGCTCCCTGAGCTCAGAGTCATCCTGGTATGACTCCCCCTGGGGCAACGCTGGG GAGGTGAGCGAGGTGGAGGGCTCCTTCCTGGCTCCCAGCACTCCAGACCCCAGCCTCCCCAGCAGCTTCC CACCCAGTGACACCAAAAAGCCTTTCAACCAAAGCTCTTCCCTCTCCTCCCTCCGGGAATTGTACAAAGA TCCCAACCTGGGGTGCCGCTCACCTTCCGGCACCTGCCTTTCTTCCAATGAGTACATCAGCTCTCAAGTC AGCCTGAACAACCGAGTCTCCTTTGCGTCTGACATGGATGTGCCCTCCAGGGTGGATCACAGGGATCCCC TGCACTATAGTTCCTTTACTCTCCCCTGTCGCAAGTCCAAAGCCTTAACTGAAGATGCAGCTAAGAAAGA CACCCTCAAAGCCAGAATGCGGCGCTTCAGTGACTGGACAGGAAGCCTCTCCAGGAAGAAGAGGAAACTG CAGGAACCCAGGTCCATGGAGGGCAGTGAGTACTTTGATAGCCACTCAGATGGACTGAATGCAGAAGGGC AGGTGCCCGCGCAGACATCTTCCTTACTGTGGTCAGGGGGCTCGGCTCAGACCCTGCCTCACAGAAGCGA ATCCACTCACGCTATCAGCGTCGATCCCCTCCGACAGAACATCTATGAGAATTTCATGCGAGAGCTCGAA ATGAGCAGGAGCAACACAGAACACGTGGAAACTTCCACAGAGACCATGGAGTCCAGCAGCGAGTCTGTCA GCTCACTGGAGCAGCTGGATCTGCTCTTTGAGAAGGAGCAGGGAGTGGTCCGGAAAGCCGGGTGGCTCTT CTTCAAACCCCTTGTCACCTTGCAGAAGGAGAGGAAACTGGAGCTGGTGGCTCGGAGGAAGTGGAAACAA TACTGGGTGACCCTGAAAGGCTGTACTCTGCTGTTTTATGAGACCTATGGAAAGAATTCCACAGAGCAGA ATAGTGCCCCACGGTGTGCCCTCTTTGCAGAGGACAGCATCGTGCAGTCTGTCCCAGAGCATCCCAAGAA GGAACACGTGTTCTGCCTGAGTAACTCCTGTGGGGACGTCTACCTATTCCAGGCCACTAGCCAGACAGAT CTGGAAAACTGGGTCACAGCCATCCACTCGGCGTGCGCATCCCTCTTTGCAAAGAAGCACGGAAAGGAGG ACACGGTGCGACTGCTAAAGAGCCAGACCAGAAGCCTGCTTCAGAAGATAGACATGGATAGCAAGATGAA GAAGATGGCAGAGTTGCAGCTGTCTGTGGTGAGCGACCCCAAGAACAGGAAGGCCATCGAGAATCAGATC CGGCAATGGGAGCAGAATCTGGAAAAATTCCACATGGACCTGTTCCGCATGCGCTGCTATTTGGCGAGCT TACAAGGTGGGGAGTTACCAAATCCCAAGAGTCTCCTTGCTGCCACCAGCCGCCCCTCCAAGCTGGCTCT TGGCAGGCTGGGCGTCCTGTCTGTTTCGTCTTTCCATGCTCTGGTGTGTTCCAGAGATGATTCCACTCTC AGGAAAAGAACACTTTCCCTTACCCAGAGAGGAAAAAGCAAGAAAGGCATATTTTCTTCATTGAAAGGTC TGGACACCCTAGCAAGAAAGGGTAGGGAGAAGAGAGCTTCCATAACTCAGATGTTTGATTCTAGCCACAG CCATGGATTTCTTGGAACTCAGCTACCTCAAAAGTCCACTAACTCCAACAAGGCCCATGACCTGCATCTG TATGGCTCCGCAGTAGACAGCGCGCTGCGAGACAGCATGTGGGAAGTCCAGACTTATGTCCACTTCCAGG ATAACGAAGGAGTTACTGTGACCATCAAGCCAGAGCACAGGGTGGAAGATGTTCTGGCTTTGGTGTGCAA GATGAGACAGTTGGAACCCACTCACTATGGTCTTCAGCTCCGAAAGGTGGTCGATAAAAGTGTGGAGTGG TGTGTGCCCGCGCTGTATGAATACATGCAAGAGCAGGTTTATGATGAAATCGAAGTTTTCCCACTCAGTG TGTATGACGTGCAGCTAACCAAGACTGGGGACATGACTGACTTTGGGTTTGCAGTCACAGTTCAGGTGGA CGAACACCAGCATCTCAACCGGATATTTATCAGTGATGTTCTCCCTGACAGCCTGGCATACGGAGGAGGG CTGAGAAAGGGCAACGAAATCACAAGCTTAAATGGGGAACCAGTGTCTGACCTTGACATCCAGCAGATGG AGGCTTTGTTTTCTGAGAAGAGCGTTGGCCTCACTCTAGTCGCCCGGCCTGTGACCACAAGACGAACCCT GTGTGCTTCCTGGTCAGACAGTGACCTGTTCTCCAGGGACCAGAAAAGTCTGCCGCCCTCTCCCAACCAG TCCCAGCTGCTGGAGGAATTCCTGGATAACTTTAGAAAAACCGCCACGAGTGATTTCAGCAATGTCCCTG AGATCACAACTGGCTTGAAGAGGAGCCAGACAGAAGGCACCCTGGATCAAGTGCCCCACAGGGAAAAGAT GGAGCAGACATTCCTGAGCGCTGACCAGATTGCGGAGCTCTGCAGGGACTTGAACAACACCCACACCAAC AGTATGGAAGCACCAACAGAGAGCCATGACCCACCTCCCAGGCCTCTGGCTCGTCACCTCTCAGATGCAG ATCGCCTCCGGAAAGTCATCCAGGAGCTTGTAGACACAGAGAAGTCTTACGTGAAGGATCTGAGCTGCCT CTTTGAACTATACTTGGAGCCACTTCAGAATGAGACCTTTCTTACCCAAGATGAGATGGAGTCACTTTTT GGGAGCCTGCCAGAGATGCTGGAGTTTCAAAAGGTGTTCCTGGAGACGTTGGAGGATGCGATCTCCGCTT CCTCGGACTTTAGTGTCCTGGAAACCCCCTCACAGTTTCGAAAATTGCTGTTCTCCCTTGGAGGTTCTTT CCTCTACTATGCGGATCACTTTAAGCTATACAGTGGGTTCTGTGCCAACCACATTAAAGTACAGAGGGTT CTAGAGCGAGCTAAAACGGACAAGGCCTTCAAGGCTTTTCTGGATGCCCGAAATCCCACCAAGCAGCACT CCTCCACGCTGGAGTCCTATCTCATCAAGCCTGTTCAGAGAGTGCTCAAGTATCCTCTGCTTCTCAAGGA GCTAGTGTCACTGACTGACCATGAGAGTGAAGAACACTATCACCTGACAGAAGCACTAAAGGCCATGGAA AAAGTCGCCAGTCACATCAATGAGATGCAGAAGATCTACGAGGATTACGGGATGGTGTTTGACCAGCTGG TGGCAGAGCAGAGTGGCACAGAGAAGGAAGTGACAGAGCTGTCCATGGGGGAACTTCTGATGCACTCTAC AGTTTCCTGGTTGAATCCGTTCCTGTCTCTAGGAAAAGCCAGGAAGGACATTGAGCTCACAGTATTTGTT TTTAAGAGAGCTGTCATACTGGTTTATAAAGAAAACTGCAAGCTGAAAAAGAAACTGCCCTCGAATTCCC GGCCTGCTCACAACTCTGCTGACTTGGATCCATTTAAATTCCGCTGGTTGATTCCCATATCTGCGCTTCA AGTTAGACTGGGGAACACGGCAGGGACTGAAAATAATTCCACGTGGGAGCTGATTCATACCAAGTCGGAA ATTGAAGGACGGCCAGAAACCATCTTTCAACTGTGCTGCAGTGACAGCGAGAACAAAACCAGCATTGTTA AGGTGATTCGTTCTATTCTGAGAGAGAACTTCCGGCGCCACATAAAGTGTGAGCTGCCACTGGAGAAGAC GTGTAAGGACCGGCTAGTACCTCTTAAGAACCGAGTTCCTGTTTCAGCCAAATTAGCCTCGTCCAGGTCG TTGAAGGGCCTCAGAACATCCTCCAGCAGCGAGTGGCCCAGCGAGCCCAGCAAGGGCAACTCACTGGACT CAGATGAGTGCAGCCTGAGCAGTGGCACCCAGAGTAGCGGCTGCCCCGTAGCCGAGAGCAGGCGAGACTC TAAGAGCACCGAGCTGGAGAAAGACGCTCAGGAGGGCCTGGCGGAGTTTCCAGATGGTCTTATCAAAGAA AGCGACATTCTGAGTGATGAAGATGAGGACTTCCACCACCCTCTGAAACAGGGTAGCCCTACTAAGGACA TTGAGATTCAGTTCCAGAGACTGAAAATCTCTGAGGAATCCGACGTGCACCCAGTTGGGCAGCAGCCTCT CACAGAGTCAGGTGAACAGCCCAAGCTGGTCAGGGGCCATTTTTGCCCCATTAAACGGAAAGCAAACAGC ACCAAGAGGGGCAGAGGAACTTTGCTCAAGGCGCAGACTCGTCACCAGTCCCTGGACAGCCACCCAGAAA CTGCCAGCATTGATCTAAACTTGGTCCTGGAGAGAGAATTCAGTGTCCAGAGCTTAACTTCAGTCGTCAA TGAGGAGGGTTTTTATGAAACACAGAGCCATGGCAAATCATGATTTGTGTAGTGTGCTTACAATGCTCAT TTGTTTTCAAATTGGTGGAAAAGAGAAACTGCAGGAAAACTATTCATGTTTGAGTTTTACGCAGTATATT TTTTCCACAAAACCTGTAAAGATTTAAGTTATTTTAATTTATTGTGAATCCGAAAACTAGATTTAGTTGG TCAGAATCTGTAAAGTACTAGCACCTATCCCTTTGGAGCAGCAATCAAATGATTTAGTCTTGTAATAAAA TTGCATTTTAATTATCTTAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6853027 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6853027 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13150 same embryo
	EMAGE:13149 same embryo
	EMAGE:13148 same embryo
	EMAGE:13145 same embryo
	EMAGE:13146 same embryo
	EurExpress:euxassay_017276 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source