EMAGE mouse gene expression spatial database

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EMAGE:13149

Col4a1 collagen, type IV, alpha 1 ( MGI:88454)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_017239_01	euxassay_017239_02	euxassay_017239_03	euxassay_017239_04	euxassay_017239_05

euxassay_017239_06	euxassay_017239_07	euxassay_017239_08	euxassay_017239_09	euxassay_017239_10

euxassay_017239_11	euxassay_017239_12	euxassay_017239_13	euxassay_017239_14	euxassay_017239_15

euxassay_017239_16	euxassay_017239_17	euxassay_017239_18	euxassay_017239_19	euxassay_017239_20

euxassay_017239_21	euxassay_017239_22	euxassay_017239_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13149_wholemount_strong.wlz

13149_wholemount_moderate.wlz

13149_wholemount_weak.wlz

13149_wholemount_possible.wlz

13149_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13149_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

lateral ventricle choroid plexus

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

testis

strong

regional

strong expression: see section 06 07 08 09 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9357
Entity Detected:	Col4a1, collagen, type IV, alpha 1 ( MGI:88454)
Sequence:	sense strand is shown >T9357 CGAGACTGAGCACCTCGCCGTGGCCCACGGGAACTGGGCTGCATTTCCCCGCCGTCTCTGCTGGTCCCCT GCCGCCCCGGGCGCGCCACCATGGGGCCCCGGCTCAGCGTCTGGCTTCTGCTGCTCTTCGCCGCCCTTCT GCTCCACGAGGAGCGCAGCCGAGCAGCTGCGAAGGGCGATTGTGGTGGCTCTGGCTGTGGAAAATGTGAC TGTCATGGCGTGAAGGGACAAAAGGGAGAAAGAGGCTTGCCAGGGTTGCAAGGTGTCATTGGATTTCCCG GGATGCAAGGACCTGAGGGGCCACATGGACCACCAGGACAAAAGGGTGATGCTGGAGAACCTGGACTTCC TGGCACAAAAGGGACGAGGGGACCCCCTGGAGCAGCTGGCTACCCTGGGAATCCCGGACTTCCTGGTATT CCTGGCCAAGATGGCCCTCCAGGTCCCCCAGGTATCCCAGGATGCAACGGTACAAAGGGAGAGAGAGGGC CGCTCGGTCCTCCTGGCTTGCCTGGATTCAGCGGCAATCCTGGACCACCAGGGTTACCAGGAATGAAGGG AGATCCTGGTGAAATTCTCGGCCACGTTCCCGGAACACTGCTGAAAGGGGAGAGAGGATTTCCTGGTATC CCCGGGATGCCGGGCTCACCAGGGTTGCCAGGACTCCAGGGACCCGTGGGTCCTCCGGGATTTACTGGAC CACCGGGTCCTCCAGGCCCTCCTGGACCTCCTGGAGAAAAGGGCCAGATGGGCTCCAGCTTCCAAGGACC AAAAGGTGACAAGGGAGAGCAAGGGGTCAGCGGTCCCCCGGGAGTTCCTGGACAGGCACAAGTTAAGGAA AAAGGAGACTTTGCCCCAACAGGAGAAAAGGGTCAGAAAGGTGAACCTGGATTTCCGGGAGTACCAGGAT ATGGAGAGAAAGGTGAACCTGGCAAGCAAGGGCCTCGGGGAAAACCTGGGAAAGACGGTGAAAAAGGAGA AAGGGGGAGCCCGGGCATTCCTGGCGATTCCGGGTACCCAGGTCTCCCAGGCCGGCAGGGCCCGCAGGGA GAGAAGGGTGAAGCTGGACTTCCAGGCCCCCCTGGAACTGTGATAGGCACCATGCCTTTGGGAGAGAAAG GAGATCGAGGCTACCCAGGAGCACCAGGGTTGAGAGGAGAGCCCGGCCCTAAAGGTTTCCCTGGAACACC AGGCCAACCAGGCCCTCCAGGTTTCCCTACTCCAGGCCAGGCTGGTGCTCCAGGCTTCCCAGGTGAAAGG GGAGAAAAAGGTGACCAGGGATTTCCAGGCGTGTCATTGCCAGGACCAAGTGGAAGAGATGGAGCCCCGG GGCCTCCCGGCCCTCCCGGCCCCCCTGGGCAGCCAGGCCACACAAATGGCATTGTGGAGTGTCAACCTGG ACCACCCGGGGACCAGGGCCCTCCTGGGACTCCAGGACAGCCAGGTTTGACAGGTGAAGTTGGACAGAAA GGTCAGAAAGGAGAGAGCTGCCTTGCCTGTGACACAGAAGGACTTCGTGGCCCTCCAGGGCCACAGGGGC CTCCGGGAGAGATTGGTTTCCCTGGACAACCTGGGGCTAAAGGTGACCGAGGCCTGCCCGGCAGAGATGG TCTTGAAGGATTGCCGGGTCCACAAGGTTCACCAGGGCTTATAGGCCAGCCTGGAGCTAAGGGAGAGCCT GGAGAGATATTTTTTGACATGCGACTCAAAGGTGACAAAGGAGACCCAGGTTTTCCGGGACAGCCTGGCA TGCCAGGAAGAGCAGGAACTCCCGGAAGAGATGGCCACCCAGGACTCCCTGGACCCAAAGGCTCTCCGGG TTCAATAGGATTGAAAGGAGAGCGAGGTCCTCCCGGAGGAGTTGGCTTCCCTGGTAGTCGTGGAGACATC GGCCCTCCTGGACCCCCAGGAGTTGGCCCCATTGGTCCTGTTGGTGAAAAAGGACAAGCAGGCTTTCCTG GAGGCCCTGGGTCCCCAGGTCTTCCAGGCCCAAAGGGTGAGGCAGGAAAGGTTGTCCCACTCCCTGGCCC CCCAGGAGCTGCAGGACTTCCAGGATCCCCTGGCTTCCCAGGGCCACAAGGTGACCGAGGCTTCCCAGGA ACCCCAGGACGTCCAGGCATCCCGGGAGAGAAAGGTGCTGTGGGCCAGCCAGGAATTGGATTTCCTGGGC TTCCTGGCCCCAAAGGTGTTGATGGCTTGCCTGGAGAGATAGGACGGCCTGGGAGTCCAGGTCGCCCTGG ATTTAACGGCTTACCTGGCAACCCAGGACCTCAAGGTCAAAAGGGAGAACCTGGCATTGGGCTTCCAGGA CTCAAAGGGCAACCAGGCCTTCCAGGCATTCCCGGTACACCTGGAGAGAAGGGTAGCATCGGGGGACCCG GCGTTCCAGGAGAACAGGGGTTGACAGGCCCCCCAGGACTCCAGGGGATCAGAGGTGACCCAGGGCCTCC TGGAGTTCAAGGCCCAGCAGGTCCACCAGGGGTCCCAGGAATAGGGCCACCCGGAGCTATGGGCCCTCCC GGAGGGCAAGGACCACCAGGGTCATCAGGTCCACCTGGAATTAAAGGAGAGAAAGGGTTCCCTGGATTCC CCGGACTGGATATGCCTGGCCCCAAAGGCGATAAAGGCTCTCAAGGACTTCCTGGCCTCACAGGACAGTC AGGCCTCCCTGGCCTTCCTGGACAGCAGGGGACACCTGGAGTTCCAGGGTTCCCAGGTTCTAAAGGTGAA ATGGGTGTCATGGGAACCCCGGGACAACCAGGCTCGCCAGGACCAGCAGGCACCCCAGGGTTACCTGGAG AAAAAGGGGACCATGGCCTTCCGGGCTCCTCAGGACCCAGGGGCGACCCTGGCTTCAAAGGTGATAAAGG TGACGTTGGGCTTCCTGGCATGCCAGGATCCATGGAGCATGTGGACATGGGGAGCATGAAGGGACAGAAA GGAGACCAGGGAGAGAAAGGACAAATCGGACCCACTGGTGATAAAGGTTCCCGAGGAGACCCTGGAACAC CAGGAGTACCTGGGAAGGATGGGCAGGCAGGGCATCCCGGACAGCCAGGGCCTAAAGGTGACCCAGGCCT TAGTGGGACACCAGGATCCCCTGGACTCCCTGGACCCAAAGGATCAGTTGGAGGAATGGGCTTGCCAGGT TCGCCTGGAGAAAAAGGTGTGCCTGGCATCCCTGGCTCACAGGGTGTCCCTGGCTCACCTGGAGAGAAGG GAGCCAAAGGAGAGAAAGGGCAGTCAGGTCTACCTGGCATTGGGATTCCGGGACGGCCTGGTGACAAGGG AGACCAGGGCCTTGCTGGCTTCCCAGGCAGCCCCGGTGAGAAGGGAGAGAAAGGCAGTGCCGGAACCCCA GGGATGCCAGGGTCCCCAGGCCCGAGAGGTTCTCCGGGGAACATCGGCCATCCAGGAAGCCCAGGCCTGC CTGGAGAGAAAGGGGATAAAGGCCTCCCAGGACTGGATGGCGTTCCTGGTGTCAAAGGAGAAGCAGGTCT CCCTGGGACTCCTGGCCCCACAGGCCCAGCTGGCCAGAAGGGAGAGCCGGGCAGCGATGGAATCCCGGGG TCGGCAGGAGAGAAGGGTGAACAAGGTGTTCCAGGAAGAGGCTTCCCCGGCTTCCCTGGCTCCAAAGGAG ACAAAGGCTCCAAGGGTGAAGTGGGTTTCCCTGGCCTAGCTGGAAGTCCTGGAATTCCTGGAGTCAAAGG CGAGCAAGGGTTCATGGGTCCTCCTGGCCCTCAAGGACAACCTGGCTTACCTGGCACTCCTGGTCACCCT GTGGAGGGGCCCAAAGGAGACCGAGGACCTCAGGGTCAACCTGGCCTGCCAGGGCATCCGGGACCTATGG GGCCGCCAGGGTTCCCAGGAATCAATGGGCCAAAAGGTGACAAGGGAAATCAAGGTTGGCCAGGAGCTCC GGGGGTTCCAGGCCCTAAGGGAGACCCAGGATTCCAAGGCATGCCGGGCATTGGCGGCTCTCCAGGGATC ACAGGTTCAAAGGGAGATATGGGACTGCCCGGAGTTCCAGGATTTCAAGGTCAGAAAGGGCTTCCTGGTC TGCAGGGAGTGAAAGGAGACCAGGGAGACCAAGGTGTACCCGGCCCTAAAGGTCTCCAAGGTCCCCCTGG GCCTCCAGGTCCCTACGATGTCATCAAAGGAGAACCAGGGCTCCCTGGTCCTGAGGGTCCCCCTGGTCTT AAAGGACTCCAGGGACCACCAGGTCCAAAAGGACAGCAAGGTGTGACAGGCTCAGTGGGCTTGCCTGGAC CTCCAGGTGTCCCTGGGTTCGATGGTGCTCCTGGCCAGAAAGGAGAGACAGGACCCTTTGGACCACCTGG TCCAAGAGGGTTTCCTGGCCCACCAGGCCCCGATGGGCTGCCAGGATCCATGGGTCCCCCAGGTACCCCA TCTGTGGACCATGGCTTCCTTGTGACCAGGCATAGTCAGACAACAGATGACCCACTGTGTCCCCCAGGGA CCAAAATTCTTTACCATGGATACTCTCTGCTCTATGTCCAAGGCAACGAGCGTGCCCACGGGCAGGACTT GGGTACGGCTGGCAGCTGCCTGCGTAAGTTCAGCACCATGCCCTTTCTCTTCTGCAACATCAACAACGTC TGCAACTTCGCCTCCAGGAACGACTACTCTTACTGGCTGTCCACGCCAGAGCCCATGCCCATGTCCATGG CACCCATCTCTGGGGACAACATCCGGCCCTTCATTAGCAGCTTTCCTTGGAAAACAGGTGTGCGGTTTGT GAAGCACCGGCCATGGTGATGGCGGTACACAGTCAGACCATTCAGATTCCGCAGTGCCCTAACGGTTGGT CCTCACTGTGGATCGGCTATTCCTTCGTGATGCACACCAGCGCTGGTGCTGAAGGTTCCGGCCAAGCCCT CGCATCCCCCGGGTCCTGTCTGGAAGAGTTTAGAAGCGCCCCATTCATCGAGTGCCACGGCAGAGGAACG TGCAATTACTACGCAAATGCTTACAGCTTTTGGCTCGCCACCATAGAGAGAAGCGAGATGTTCAAGAAGC CCACGCCATCCACCTTGAAGGCAGGGGAGCTGCGAACACACGTCAGCCGCTGCCAAGTGTGCATGAGAAG AACATAACTATGATGCTCGCCTCTGCCACCGTCACAACATGGTGCTACTCCCCCCCCTTGTCCTCTTCTC CCTCCCATTGTATTTGTAACAGCAATGAACCCTAGAATTATATCCTGTGTACCTCACTGTCCACTATGAA AACCATAAAGTGCCTTATAGGGATTCGCGTAACTAACACACCCCGCTTCGTTGGCCTCTGTTTGCTGAAG GAGAAGGACAGGCAGCGGAGAGCTTTCCATAATGGCACATACCAAATGGCGCTCCTGATCCAATAAAAGA TGAATTGCATTTTGCAATCCAGTGCACTGATGTCTGGGGCGAGAACTCCATAAGAAACCCAAAGGTGCTA GGGGCGTGAGGCGCTCTCCACACAGCCCACAGCCCGCTTCATCCTGTCACCCCAGATGTGAGTGCTGTCC AACCCACTGTCCAGTGTTTCCACATCAGGTCCCTCGAGGGGCTATGCAGAGAACAGTCAAGGGAACTGGT TTCTTCCACTTTCTACATGGAACTCCATCGTTTCAGCCCCAAACACAAACTTATGCCCTGTGTGGTACTA TGCAGCTGCTTTTGTGGAAGTCATGGCTTTCCTGTGGAATAAAGATGGTCCAAAGGTTAGATGGAAAATA AATAAATAAGTAAATAAATATATATATATATTAGTAATTTATATAGGTGTCAGCAATTAGGCAGGTCAAG TTCTAGCGTAGAGCCTGTTGTTAAATAAAGCGCACTTGGTTTTCGTCTCTGGAGAAACTGTACTATCCTT TCACAGGGTTACCAACATCAGGCCACTGAATGTGAAACACCAGCTTTCCTCAAACTCACTCCTAAACCAG AGGAGGTGTATAGATAGCCAAAGCCAAACCCATTCCTAAACATGGTGTCTGATAATGAGCCCTGTGCCTG GCGCTTCTTGCTTCTGCTCTGCGTGGAGTATTTTCAGGGTTTGCAACACTAACCACAGACTGAATGACTG ACTTCCCGTACGACAGCCAAGGCCTTTGTTAGCCTCCTTCATCCTGCATCCTGGCAGGCATTTCACACCT GAGCACACACAGGCAACTTCCCAGCTCCTCTCGAGCTGTGACACAGCCCCTGCAGGGAGTGTACAGGTCG CAGGAGGGCAGACTCGGACCACTATGCTTGAAGTGACATTTCACTAAAAGTACCCACCCTCCAAAAAAAA AAAAAAAAGTTTTTAAGACTACTAAGGCCTTCTGTGTAATTTCTTTAAATGTGTATTTCTTAAGAATTCA AATTTGTAATAAAACTATTTGTATAAAAATGAAGCTTTTATGGATTTGTTGCTAGAAATGAAACGTTGAA AGAGAAGTTACCACACGTACCACTAATAAACCTGTAAGCTTTGCTGCAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6852661 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6852661 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13150 same embryo
	EMAGE:13148 same embryo
	EMAGE:13145 same embryo
	EMAGE:13146 same embryo
	EMAGE:13147 same embryo
	EurExpress:euxassay_017239 same experiment
	MGI:4823991 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source