EMAGE mouse gene expression spatial database

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EMAGE:13403

Hif1a hypoxia inducible factor 1, alpha subunit ( MGI:106918)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017476. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017476_01	euxassay_017476_02	euxassay_017476_03	euxassay_017476_04

euxassay_017476_05	euxassay_017476_06	euxassay_017476_07	euxassay_017476_08	euxassay_017476_09

euxassay_017476_10	euxassay_017476_11	euxassay_017476_12	euxassay_017476_13	euxassay_017476_14

euxassay_017476_15	euxassay_017476_16	euxassay_017476_17	euxassay_017476_18	euxassay_017476_19

euxassay_017476_20	euxassay_017476_21	euxassay_017476_22	euxassay_017476_23	euxassay_017476_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13403_wholemount_strong.wlz

13403_wholemount_moderate.wlz

13403_wholemount_weak.wlz

13403_wholemount_possible.wlz

13403_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13403_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7125
Entity Detected:	Hif1a, hypoxia inducible factor 1, alpha subunit ( MGI:106918)
Sequence:	sense strand is shown >T7125 CACGCGTCCGGGGGACGCGAGGACTGTCCTCGCCGCCGTCGCGGGCAGTGTCTAGCCAGGCCTTGACAAG CTAGCCGGAGGAGCGCCTAGGAACCCGAGCCGGAGCTCAGCGAGCGCAGCCTGCAGCTCCCGCCTCGCCG TCCCGGGGGGCGTCCCGCCTCCCACCCCGCCTCTGGACTTGTCTCTTTCTCCGCGCGCGCGGACACAGCC GGCGCTTAGGCCCGAGCGAGCCCGGGGGCCGCCGGCCGGGAAGACAACGCGGGCACCGATTCGCCATGGA GGGCGCCGGCGGCGAGAACGAGAAGAAAAAGATGAGTTCTGAACGTCGAAAAGAAAAGTCTAGAGATGCA GCAAGATCTCGGCGAAGCAAAGAGTCTGAAGTTTTTTATGAGCTTGCTCATCAGTTGCCACTTCCCCACA ATGTGAGCTCACATCTTGATAAAGCTTCTGTTATGAGGCTCACCATCAGTTATTTACGTGTGAGAAAACT TCTGGATGCCGGTGGTCTAGACAGTGAAGATGAGATGAAGGCACAGATGGACTGTTTTTATCTGAAAGCC CTAGATGGCTTTGTGATGGTGCTAACAGATGACGGCGACATGGTTTACATTTCTGATAACGTGAACAAAT ACATGGGGTTAACTCAGTTTGAACTAACTGGACACAGTGTGTTTGATTTTACTCATCCATGTGACCATGA GGAAATGAGAGAAATGCTTACACACAGAAATGGCCCAGTGAGAAAAGGGAAAGAACTAAACACACAGCGG AGCTTTTTTCTCAGAATGAAGTGCACCCTAACAAGCCGGGGGAGGACGATGAACATCAAGTCAGCAACGT GGAAGGTGCTTCACTGCACGGGCCATATTCATGTCTATGATACCAACAGTAACCAACCTCAGTGTGGGTA CAAGAAACCACCCATGACGTGCTTGGTGCTGATTTGTGAACCCATTCCTCATCCGTCAAATATTGAAATT CCTTTAGATAGCAAGACATTTCTCAGTCGACACAGCCTCGATATGAAATTTTCTTACTGTGATGAAAGAA TTACTGAGTTGATGGGTTATGAGCCGGAAGAACTTTTGGGCCGCTCAATTTATGAATATTATCATGCTTT GGATTCTGATCATCTGACCAAAACTCACCATGATATGTTTACTAAAGGACAAGTCACCACAGGACAGTAC AGGATGCTTGCCAAAAGAGGTGGATATGTCTGGGTTGAAACTCAAGCAACTGTCATATATAATACGAAGA ACTCCCAGCCACAGTGCATTGTGTGTGTGAATTATGTTGTAAGTGGTATTATTCAGCACGACTTGATTTT CTCCCTTCAACAAACAGAATCTGTGCTCAAACCAGTTGAATCTTCAGATAAGAAGATGACTCAGCTGTTC ACCAAAGTTGAATCAGAGGCTACAAGCTGCCTTTTTGATAAGCTTAAGAAGGAGCCTGATGCTCTCACTC TGCTGGCTCCAGCTGCCGGCGACACCATCATCTCTCTGGATTTTGGCAGCGATGACACAGAAACTGAAGA TCAACAACTTGAAGATGTTCCATTATATAATGATGTAATGTTTCCCTCTTCTAATGAAAAATTAAATATA AACCTGGCAATGTCTCCTTTACCTTCATCGGAAACTCCAAAGCCACTTCGAAGTAGTGCTGATCCTGCAC TGAATCAAGAGGTTGCATTAAAATTAGAATCAAGTCCAGAGTCACTGGGACTTTCTTTTACCATGCCCCA GATTCAAGATCAGCCAGCAAGTCCTTCTGATGGAAGCACTAGACAAAGTTCACCTGAGAGACTTCTTCAG GAAAACGTAAACACTCCTAACTTTTCCCAGCCTAACAGTCCCAGTGAATATTGCTTTGATGTGGATAGCG ATATGGTCAATGTATTCAAGTTGGAACTGGTGGAAAAACTGTTTGCTGAAGACACAGAGGCAAAGAATCC ATTTTCAACTCAGGACACTGATTTAGACTTGGAGATGCTGGCTCCCTATATCCCAATGGATGATGATTTC CAGTTACGTTCCTTTGATCAGTTGTCACCATTAGAGAGCAATTCTCCAAGCCCTCCAAGTATGAGCACAG TTACTGGGTTCCAGCAGACCCAGTTACAGAAACCTACCATCACTGCCACTGCCACCACAACTGCCACCAC TGATGAATCAAAAACAGAGACGAAGGACAATAAAGAAGATATTAAAATACTGATTGCATCTCCATCTTCT ACCCAAGTACCTCAAGAAACGACCACTGCTAAGGCATCAGCATACAGTGGCACTCACAGTCGGACAGCCT CACCAGACAGAGCAGGAAAGAGAGTCATAGAACAGACAGACAAAGCTCATCCAAGGAGCCTTAACCTGTC TGCCACTTTGAATCAAAGAAATACTGTTCCTGAGGAAGAATTAAACCCAAAGACAATAGCTTCGCAGAAT GCTCAGAGGAAGCGAAAAATGGAACATGATGGCTCCCTTTTTCAAGCAGCAGGAATTGGAACATTATTGC AGCAACCGGGTGACTGTGCACCTACTATGTCACTTTCCTGGAAACGAGTGAAAGGATTCATATCTAGTGA ACAGAATGGAACGGAGCAAAAGACTATTATTTTAATACCCTCCGATTTAGCATGCAGACTGCTGGGGCAG TCAATGGATGAGAGTGGATTACCACAGCTGACCAGTTACGATTGTGAAGTTAATGCTCCCATACAAGGCA GCAGAAACCTACTGCAGGGTGAAGAATTACTCAGAGCTTTGGATCAAGTTAACTGAGCGTTTCCTAATCT CATTCCTTTTGATTGTTAATGTTTTTGTTCAGTTGTTGTTGTTTGTTGGGTTTTTGTTTCTGTTGGTTAT TTTTGGACACTGGTGGCTCAGCAGTCTATTTATATTTTCTATATCTAATTTTAGAAGCCTGGCTACAATA CTGCACAAACTCAGATAGTTTAGTTTTCATCCCCTTTCTACTTAATTTTCATTAATGCTCTTTTTAATAT GTTCTTTTAATGCCAGATCACAGCACATTCACAGCTCCTCAGCATTTCACCATTGCATTGCTGTAGTGTC ATTTAAAATGCACCTTTTTATTTATTTATTTTTGGTGAGGGAGTTTGTCCCTTATTGAATTATTTTTAAT GAAATGCCAATATAATTTTTTAAGAAAGCAGTAAATTCTCATCATGATCATAGGCAGTTGAAAACTTTTT ACTCATTTTTTTCATGTTTTACATGAAAATAATGCTTTGTCAGCAGTACATGGTAGCCACAATTGCACAA TATATTTTCTTTAAAAAACCAGCAGTTACTCATGCAATATATTCTGCATTTATAAAACTAGTTTTTAAGA AATTTTTTTTGGCCTATGGAATTGTTAAGCCTGGATCATGAAGCTGTTGATCTTATAATGATTCTTAAAC TGTATGGTTTCTTTATATGGGTAAAGCCATTTACATGATATAAAGAAATATGCTTATATCTGGAAGGTAT GTGGCATTTATTTGGATAAAATTCTCAATTCAGAGAAGTTATCTGGTGTTTCTTGACTTTACCAACTCAA AACAGTCCCTCTGTAGTTGTGGAAGCTTATGCTAATATTGTGTAATTGATTATGAAACATAAATGTTCTG CCCACCCTGTTGGTATAAAGACATTTTGAGCATACTGTAAACAAACAAACAAAAAATCATGCTTTGTTAG TAAAATTGCCTAGTATGTTGATTTGTTGAAAATATGATGTTTGGTTTTATGCACTTTGTCGCTATTAACA TCCTTTTTTCATATAGATTTCAATAAGTGAGTAATTTTAGAAGCATTATTTTAGGAATATAGAGTTGTCA TAGTAAACATCTTGTTTTTTCTATGTATACTGTATAAATTTTTCGTTCCCTTGCTCTTTGTGGTTGGGTC TAACACTAACTGTACTGTTTTGTTATATCAAATAAACATCTTCTGTGGACCAGGCCCCCTGGGTCAGCGC TTACGTTTAAATAACATTTGTCTAAACATTTCTAGCTCATAAAACGATTTCTCAAAAATTTAAGTTCTTT ATAAAAATTAGATTGTACATTTCTACATTCATTTTATTGCCATTTTCTAATGTATGATGTGTCCCTAAAT GTCATGTTAAATAATGACATCATAATATTGCATTGTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4019056 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4019056 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13406 same embryo
	EMAGE:13405 same embryo
	EMAGE:13404 same embryo
	EurExpress:euxassay_017476 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source