EMAGE mouse gene expression spatial database

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EMAGE:14000

Vps37b vacuolar protein sorting 37B (yeast) ( MGI:1916724)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001738. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001738_01	euxassay_001738_02	euxassay_001738_03	euxassay_001738_04

euxassay_001738_05	euxassay_001738_06	euxassay_001738_07	euxassay_001738_08	euxassay_001738_09

euxassay_001738_10	euxassay_001738_11	euxassay_001738_12	euxassay_001738_13	euxassay_001738_14

euxassay_001738_15	euxassay_001738_16	euxassay_001738_17	euxassay_001738_18	euxassay_001738_19

euxassay_001738_20	euxassay_001738_21	euxassay_001738_22	euxassay_001738_23	euxassay_001738_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14000_wholemount_strong.wlz

14000_wholemount_moderate.wlz

14000_wholemount_weak.wlz

14000_wholemount_possible.wlz

14000_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14000_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14 15

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 weak expression: see section 24

medulla oblongata alar plate ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 15 16 17 18

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14 15

metencephalon rest of alar plate ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 18 19 20 22

pons ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19

spinal cord ventricular layer

moderate

regional

moderate expression: see section 12 13 14

vault of skull

moderate

regional

moderate expression: see section 02 03 05 09 weak expression: see section 04 24

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 05 06 17 18 weak expression: see section 04 20 22 24

viscerocranium

moderate

regional

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2694
Entity Detected:	Vps37b, vacuolar protein sorting 37B (yeast) ( MGI:1916724)
Sequence:	sense strand is shown >T2694 ATTCAGGGAAACTTTTTTTAAAATGCAGATACTGTTTTGAGCTGGTTTAAAAAATGCAGATTTATTGAGT GCCGCTTCCTGTCGGGCAAGCAGTGCACGGTCACATCTGCCAGTGCCCGTGCTGTGCCTTAGCTTCACTG GTGCCAGGACTCGGAGCCCGCGGCGTGCCAGCCGCAGCTGCCCTATGGAGCCGAAGACCAGCTAGGTAGG GCCTGGCGGGCCTCGTCACTTTGTGATGGGTCAGGAGTGGTCCATCTTCCTCCACCCCGGATAATCTCAG GAGCAATGCTGCAGGTGCTGGCGTAGGCATGGGAGGGGTTTGGGGACAGCCCGCCCCACAGGGTTGGCAG TGGCCGCTCCCTCCTCTCTGGGATGTCATCTGCAGGTGCCACATGTGCAGAGTTCCCAGGGCCCGGCTGG GGAGGGGCAGCCCCACACCTGTTCCCAGAAGAGGGCTGCGAGCCTGTTGGTAGAACTTAGAACCCCACCT TCACCCTGCCAGCTGGCTACAGCCC
Notes:	The probe template was PCR amplified from IMAGE:1512097 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1512097 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14001 same embryo
	EMAGE:14002 same embryo
	EMAGE:14004 same embryo
	EMAGE:14003 same embryo
	EMAGE:14005 same embryo
	EurExpress:euxassay_001738 same experiment
	MGI:4829172 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source