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EMAGE:14084

BC043934 cDNA sequence BC043934 ( MGI:2679715)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14084 EMAGE:14084 EMAGE:14084 EMAGE:14084 EMAGE:14084
"Pseudo-wholemount" of euxassay_001727. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001727_01 euxassay_001727_02 euxassay_001727_03 euxassay_001727_04

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14084Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14084_wholemount_strong.wlz
14084_wholemount_moderate.wlz
14084_wholemount_weak.wlz
14084_wholemount_possible.wlz
14084_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14084_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4088
Entity Detected:BC043934, cDNA sequence BC043934 ( MGI:2679715)
Sequence:sense strand is shown

>T4088
CTTTGTACTCCCGGGTGTGAGCGGTGAGGGAGTGGGCAGACCTTGTCAGTGTCCTAATCCATAGTCTCAG
GACAGGGACAGGCCAAGATGCGACAGCAATGCTGGGCATGGTTTGTTTTGCTCCAGCAGGTGTTGAACGT
GTGGCTATGCCAAAGGCCACCAGCAAACCCACACGAAGCAAACACAAAGCTTCCACTGATGGGTCTGTGG
GCTGCCGATTCTTGTGGGCTTTACAGTGCGGCAACGGCAGTGCTGGTCACATGGACCGAGAGACTGTTTT
GATGCCAGAGACTAATGCTCCGATCCGATCCGTCACCACAGGAGTTAAAGGAGCAGCCAAGAGATCAGCG
TGGTTGAACCCCCGCTCTAAGCTGCCTGCCGTTCTCTCCTCCCCTCCCACTCTGAGACTGCAGTTCACAT
GCCGAAGACAAATGGCATAAAGGGCAGGCAATTAATTTTTTCAGCTGGAGACTGATGGAATGTGGGTGTT
TGGCATGCACCTCTGTCTCCAGTCTCCTCAGGGAAATACCTCCCGGAGGGCCCTTCAAATTCCTCATCTG
CCCTTTAGGACCAATATGTAATTCAGTCCTGAAAAATCAGTCGGAATGGGTCTTGTGTCCACTATACCAA
GAGCCCAAGGACTGCACAGACGACAGGCCCATTCGTTTATTTGTGGTTTTGGTTTGTTTGTTTTTGTTTT
CTAACCCAAGGTTTCATGTAGTTTAGGCTGGCCTCAAACTCACTATGTGGACAAGGCTGGCCTTGGACTT
CTGATCCTCCTTCTTGTTGGGGTTACAGGTGTGTGTAACTGAGGGGACAGTTCGTCATGACTTTCCTTGG
AGAGAGAGAAAGGTCGTCTTAGAATATACCTCAGGGTCTAGATTCCAAGTCCCCAGAACCTCTGTTGGGT
TGGACCAGGCCAGCCAACCTGGTGAACAAAGGCAAGCAGAGCATAAAGAATTTACTGGATAGGATGCAGA
GAAGCCCTGGGGACGGGGTGCACACCTTTAATCCTAGCACTCCGGAGGCTGGGGCTGGTTGATGTCTGAG
TTCTAGGCCTACTCTGGTTGATAGAGTGAGTTCTAGAACAGCTAGGGCCACACAGAGAAACCCTGTCTTA
AAGGGGGGGGGTGGCTGAGGAAGGGGGGAACCATGCAGGGAAGTGTATTAACATAACAGCCAACAGGACC
TGGAACTGGAGAAAAACTACTGCATCCATGTTGATAGAGGTATGAAGATGTCAGAGGTAAACATACAGGC
TCAGGCTGAGCAGGGATAAAACTCAATAGTAAAACACATATCTAGCAACACGGGTTTAAGGCCCAGAGCT
GCTGCAGCTGCTGTGGCGGAGGCCGAGGCAGCTGTTGCTGTTGAAGAAGAGGAGGAGGAGGAGGAGGAGG
AAGAAGGAAGAGGAAGATGATGTAGGTCTGTTGGGATGGGCTGTAGCTGTGTGTTAAAGCATGTGTTAAG
GCTTGCCTTTGATTCCCAGAAGCGTATTAACAAGGCAGACAGGCATGGTGGCGCATTTCGGCATTTGGAA
AGCAGGAGCCAGAGGATCAAAATTTTAAAACAAGCCTGGGCTACATGAGACCCTTAAAAAGAAAACAAAT
AAAATGCATTCTTCTGTTGGAAACTTAGGAGGGTGACCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAA
Notes:The probe template was PCR amplified from IMAGE:4456300 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4456300 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14082 same embryo
 EMAGE:14083 same embryo
 EMAGE:14081 same embryo
 EMAGE:14080 same embryo
 EMAGE:14079 same embryo
 EurExpress:euxassay_001727 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS