EMAGE mouse gene expression spatial database

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EMAGE:14146

Uxs1 UDP-glucuronate decarboxylase 1 ( MGI:1915133)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001849. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001849_01	euxassay_001849_02	euxassay_001849_03	euxassay_001849_04

euxassay_001849_05	euxassay_001849_06	euxassay_001849_07	euxassay_001849_08	euxassay_001849_09

euxassay_001849_10	euxassay_001849_11	euxassay_001849_12	euxassay_001849_13	euxassay_001849_14

euxassay_001849_15	euxassay_001849_16	euxassay_001849_17	euxassay_001849_18	euxassay_001849_19

euxassay_001849_20	euxassay_001849_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14146_wholemount_strong.wlz

14146_wholemount_moderate.wlz

14146_wholemount_weak.wlz

14146_wholemount_possible.wlz

14146_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14146_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4448
Entity Detected:	Uxs1, UDP-glucuronate decarboxylase 1 ( MGI:1915133)
Sequence:	sense strand is shown >T4448 CCACGCGTCCGGCGCGGCAGGCCCCGGACCCGAGCGGTTCCCGGGCATGGTGAGCAAGGGTCTGTTGCGC CTTGTCTCTTCAGTCAACCGCAGGAGGATGAAGCTTCTGCTCGGCATCGCGCTGTTCGCCTACGCCGCCT CTGTTTGGGGCAACTTTGTTAACATGAGGTCTATCCAGGAAAATGGAGAACTAAAGATCGAAAGCAAGAT TGAAGAGATTGTTGAACCATTAAGAGAGAAAATCAGAGATTTGGAAAAAAGTTTTACCCAGAAATACCCG CCTGTAAAGTTTTTATCAGAAAAGGACCGGAAAAGGATTTTGATCACTGGAGGTGCAGGCTTTGTGGGCT CCCATCTAACTGACAAACTCATGATGGATGGCCATGAGGTGACCGTGGTGGACAACTTCTTCACAGGCAG GAAGAGAAATGTGGAACACTGGATTGGCCATGAGAACTTCGAGCTGATTAACCATGATGTGGTCGAGCCG CTCTACATAGAAGTTGACCAGATATATCATCTGGCATCTCCAGCCTCCCCACCAAACTACATGTACAACC CCATCAAGACACTGAAGACTAATACAATTGGAACACTAAACATGTTGGGATTGGCAAAGCGCGTGGGTGC CCGTCTGCTCTTGGCCTCCACTTCCGAGGTATATGGAGATCCTGAGGTCCACCCTCAAAGTGAGGACTAC TGGGGCCATGTGAATCCCATAGGACCCCGAGCCTGCTATGATGAGGGCAAACGTGTTGCAGAGACCATGT GCTATGCCTACATGAAGCAGGAAGGTGTGGAAGTACGGGTGGCAAGGATCTTCAACACCTTTGGGCCGAG AATGCACATGAATGATGGGCGGGTGGTCAGCAACTTCATCTTACAAGCACTACAAGGGGAGCCACTCACA GTCTATGGCTCTGGGTCTCAGACACGGGCATTCCAGTACGTAAGTGATCTGGTGAATGGCCTGGTAGCAC TGATGAACAGCAATGTCAGCAGTCCTGTCAACTTGGGAAATCCAGAAGAACACACAATCCTGGAATTTGC TCAGTTAATTAAAAACCTTGTTGGTAGTGGAAGTGAAATTCAGTTTCTCTCTGAAGCCCAGGATGACCCA CAGAAAAGAAAACCAGACATCAAAAAAGCAAAACTGATGCTGGGGTGGGAACCTGTGGTTCCATTGGAGG AAGGATTGAACAAAGCCATCCACTACTTCCGGAAGGAATTAGAGTACCAGGCTAATAACCAGTACATCCC CAAACCCAAGCCCGCCAGAGTGAAGAAGGGCCGGACGCGCCACAGCTGAGTTAGTTAGCCTTAGGTTATG AGACTCTGTTTTACCCGTATGAGGTGGACTTTTGTGGGATTTTTTTTTTATTTTTTAAAGACTTCAACAG GTGTCATGAAGAACAAACTGGAATTTTATTCTGAAGCTTGCTTTAAAGACACTGATGTGCCTAAACGCTC CCTTGAAAGCTGCAGACTTTGCCTTGCACTTTTAAATCTGTCTTTGTTATGTACAACAGCCTAGATGCAT CTCTGCTATTTTCAGGTTTTTATCTTCTGTTAGAGTGTATGCTGTAACTGTCATTGACAGTTTTATTTAC TGGTTTTTTTGTGAAGCTGAAAAGAAACATTAAATGGGGTGGAAAATGTCAATTTTATTTATAAAAGTGG GTACTTATAAATGAGATGTTACACTATGCATAAAGAATAAAAATCCTAGAGGTACTGTCGGGTGGGTGGG GCACCAGTGTTTGTTGGGGACAGATGAAAGGACTCAGTTGGGAAGCTTTGTCTTTCTCTTTTAATTCAGA GTTTTCTGAAGGTCTAGTTTTCAGTTGCAAACTTGACTTTGAAACATCCCTGTTGGTTCTTGATCAAAGA TATTTGAAATCACTACTGTGTTGTGCTGCATATTGGGGTGGGGGTGGGGGGACAACGTTAACATATTCTT GGTTAACCATGGTTAAATATGCTATTTTAATAAAATATTGAAACTCATCAGACATGGGTTTTGAGCTACA AAGATTGACATCTGCTTTACATCAGATGTGTGGTGGCTGAGAAGAACACTTGAGATCTTAGTTTAAGGGA ATTCTGAGAACATCCAGGTTTATACCATATTTAACTTGATATGTAACTGTTAGAACCATCTGTGTGCTCT CTGGGCTGAATCTGTGCATATGATACACACATGTGTATATGTCTTTATCTGAAATACTTAAAACCAACTG TTTCAAATTTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4935237 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4935237 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14145 same embryo
	EMAGE:14144 same embryo
	EMAGE:14142 same embryo
	EMAGE:14143 same embryo
	EurExpress:euxassay_001849 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source