EMAGE mouse gene expression spatial database

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EMAGE:14169

Mrps25 mitochondrial ribosomal protein S25 ( MGI:1928140)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003959. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003959_01	euxassay_003959_02	euxassay_003959_03	euxassay_003959_04

euxassay_003959_05	euxassay_003959_06	euxassay_003959_07	euxassay_003959_08	euxassay_003959_09

euxassay_003959_10	euxassay_003959_11	euxassay_003959_12	euxassay_003959_13	euxassay_003959_14

euxassay_003959_15	euxassay_003959_16	euxassay_003959_17	euxassay_003959_18	euxassay_003959_19

euxassay_003959_20	euxassay_003959_21	euxassay_003959_22	euxassay_003959_23	euxassay_003959_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14169_wholemount_strong.wlz

14169_wholemount_moderate.wlz

14169_wholemount_weak.wlz

14169_wholemount_possible.wlz

14169_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14169_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4942
Entity Detected:	Mrps25, mitochondrial ribosomal protein S25 ( MGI:1928140)
Sequence:	sense strand is shown >T4942 CGCTGGTCCGCGGCTTGAGGAGTTCCTGACTTCCACCATGCCGATGAAGGGTCGCTTCCCGATCCGCCGT ACGCTGCAGTACCTGGGTCGCGGGGACGTGGTATTCAAGGAGTCTGTGAAGATCATGACCGTGAACTACA ACACGTATGGGGAACTGGGCGAAGGCGCCAGGAAATTTGTGTTTTTCAACATCCCTCAGATCCAGTATAA GAATCCTTGGGTGCAGATCATGATGTTTAAGAACATGACACCATCGCCCTTCCTGCGGTTCTATCTAGAT TCTGGGGAGCAAGTCCTGGTAGACGTGGAGACGAAGAGTAATAAGGAGATCATGGAGCACATAAAGAAAA TCTTGGGCAAGAAAGAGGAGACCCTCAGGGAGGAGGAGCTGGAAAAGCAGCAGCGCTTTCATCCAGGCAA CTTTGGCCCCCGGAAGTACTGCCTGCGGGAGTGCATGTGCGAAGTAGAAGGCCAGGTGCCCTGCCCTGGC TTGGTGCCCTTACCCAAGGAGATGACAGGGAAGTACAAAGCTGCTCTGAAAGCCAGTACTTAGGTCTAAG CAAGGCCATCTGGGATGGACTGGTGGCATTTGACTGGTGGAGATGGATTACATTGGTACCATAAATGATG GAACTCTGCAATGGAAAGGCCAGCTTGCACATCTGTTTAAAGGACATGGGATGAAGGCCAGGGTTGCCAG CTGCTGAACAGATGTCAATAAAAGATTTAGTCTTCCTATACATAGTAAGTGTGGCCTTGCTGGTCTAAAG ACCATCAGCATCAGAGAGTTTGGGATGAGGAGGTGCAAGGCAGTCAGTGATTGTCTCAGTCCTGGAACTG GCCCTGCTGCTTTCACTTAATCTCCCTCCAGTTTCTACTCTGGAAGCTGTTGAGGGCTGTTGTTATTGGT GAGCCAAATGTAGCTGGGAGAGTCCATGTTCTTACTTAGCAGTCCAAAGCAAACATGTGGGCAGTGTACT AAAGCCTGCAATTCTTGCTTCCTGAGAGAGGGGAGATTCTCCAGCCCCAGTTAGTAACGAGACCTTGGGC AGTAGGATCATTTCAGGCCTGGCCTTTCAGTTCAGCAGATGTGAGCTGAAGTCACCAAATCTCTTGTCCA CTTCCTTGCCATCAAGTCCATGTGGTGGAGGAGTGCCAGTGGCATTGTGTTAACCGTAAGCATGGATTTT TCTGGCTTTGAGAATGAAGATGCCACCTGGGTGGTAATTTGACTCTCAATTCTTGCTTACTAGGTGAGAG ACAGACAAGACACACACACTCCCGGATGCCTTTTTAGGTATATTTTCCAGTTATCAGTGCCATTTTAGCT ATGTAGACTCCATTCTTCAGTGGACAACAGAATCTTCCAGGGTCACCTAATGTTTACCACAGACTTCTTG GCATTTGGCCATATAGGTATGAGTTTATGAGGCAAAATAAAGCAGTGGTTGTTAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5126320 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5126320 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14170 same embryo
	EMAGE:14171 same embryo
	EMAGE:14173 same embryo
	EMAGE:14172 same embryo
	EurExpress:euxassay_003959 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source