EMAGE mouse gene expression spatial database

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EMAGE:14172

Serpina1a serine (or cysteine) peptidase inhibitor, clade A, member 1A ( MGI:891971)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003964. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003964_01	euxassay_003964_02	euxassay_003964_03	euxassay_003964_04

euxassay_003964_05	euxassay_003964_06	euxassay_003964_07	euxassay_003964_08	euxassay_003964_09

euxassay_003964_10	euxassay_003964_11	euxassay_003964_12	euxassay_003964_13	euxassay_003964_14

euxassay_003964_15	euxassay_003964_16	euxassay_003964_17	euxassay_003964_18	euxassay_003964_19

euxassay_003964_20	euxassay_003964_21	euxassay_003964_22	euxassay_003964_23	euxassay_003964_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14172_wholemount_strong.wlz

14172_wholemount_moderate.wlz

14172_wholemount_weak.wlz

14172_wholemount_possible.wlz

14172_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14172_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 08 09 10

hindgut

strong

regional

strong expression: see section 11 12 13

rectum

strong

regional

strong expression: see section 12 13

midgut

strong

regional

strong expression: see section 11 12 13 14 15 16 17

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 24 moderate expression: see section 21 22 23

kidney calyx

moderate

regional

moderate expression: see section 06 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4943
Entity Detected:	Serpina1a, serine (or cysteine) peptidase inhibitor, clade A, member 1A ( MGI:891971)
Sequence:	sense strand is shown >T4943 CTGTCTCCTCAGCCTCGGTCACCACCCAGCTCTGGGACAGCAAGCTGAAAATGACTCCCTCCATCTCATG GGGTCTACTGCTTCTGGCAGGCCTGTGTTGCCTGGTCCCCAGCTTTCTGGCTGAGGATGTTCAGGAGACA GACACCTCCCAGAAGGATCAGTCCCCAGCCTCCCATGAGATCGCTACAAACCTGGGAGACTTTGCAATCA GCCTATACCGGGAGCTGGTCCATCAGTCCAACACTTCCAACATCTTCTTCTCCCCAGTGAGCATTGCCAC AGCCTTTGCTATGCTCTCCCTAGGGAGCAAGGGTGACACTCACACGCAGATCCTAGAGGGCCTGCAGTTC AACCTCACACAAACATCGGAGGCTGACATCCACAAGTCCTTCCAACACCTCCTCCAAACCCTCAACAGAC CAGACAGTGAGCTGCAGCTGAGCACAGGCAATGGCCTCTTTGTCAACAATGACCTGAAGCTGGTGGAGAA GTTTCTGGAAGAGGCCAAGAACCATTATCAGGCAGAAGTCTTCTCTGTCAACTTTGCAGAGTCAGAGGAG GCCAAGAAAGTGATTAATGATTTTGTGGAGAAGGGAACCCAAGGAAAGATAGCTGAGGCGGTGAAAAAAC TGGACCAAGACACAGTTTTCGCCCTGGCAAATTACATTCTCTTTAAAGGCAAATGGAAGAAGCCATTCGA TCCTGAGAACACTGAGGAAGCTGAGTTCCACGTGGACGAGTCCACCACGGTGAAGGTGCCCATGATGACC CTCTCGGGCATGCTTGATGTGCACCATTGCAGCACGCTGTCCAGCTGGGTGCTGCTGATGGATTATGCAG GCAATGCCACTGCTGTCTTCCTTCTGCCCGATGATGGGAAGATGCAGCATCTGGAGCAAACTCTCAGCAA GGAGCTCATCTCTAAGTTCCTGCTAAACAGGCGCAGAAGGTTAGCCCAGATCCACTTCCCCAGACTGTCC ATCTCTGGAGAATATAACTTGAAGACACTCATGAGTCCACTGGGCATCACCCGGATCTTCAACAATGGGG CTGACCTCTCCGGAATCACAGAGGAGAATGCTCCCCTGAAGCTCAGCCAGGCTGTGCATAAGGCTGTGCT GACCATCGATGAGACAGGAACAGAAGCTGCAGCAGTTACAGTCTTACAAATGGTTCCTATGTCTATGCCC CCTATCCTGCGCTTCGACCACCCTTTCCTTTTCATAATATTTGAAGAACACACTCAGAGCCCCATCTTTG TGGGAAAAGTGGTAGATCCCACACATAAATGACCACCCTAAAATGTCATCCTTCCTTCTGAATTGGGTTC CTTCCATTAAACACAGGCTGGCCTGGCTCGTGCCTGATGCTACAGCAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5126441 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5126441 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14170 same embryo
	EMAGE:14171 same embryo
	EMAGE:14169 same embryo
	EMAGE:14173 same embryo
	EurExpress:euxassay_003964 same experiment
	MGI:4827974 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source