EMAGE mouse gene expression spatial database

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EMAGE:14175

Osbpl9 oxysterol binding protein-like 9 ( MGI:1923784)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003963. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003963_01	euxassay_003963_02	euxassay_003963_03	euxassay_003963_04

euxassay_003963_05	euxassay_003963_06	euxassay_003963_07	euxassay_003963_08	euxassay_003963_09

euxassay_003963_10	euxassay_003963_11	euxassay_003963_12	euxassay_003963_13	euxassay_003963_14

euxassay_003963_15	euxassay_003963_16	euxassay_003963_17	euxassay_003963_18	euxassay_003963_19

euxassay_003963_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14175_wholemount_strong.wlz

14175_wholemount_moderate.wlz

14175_wholemount_weak.wlz

14175_wholemount_possible.wlz

14175_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14175_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4957
Entity Detected:	Osbpl9, oxysterol binding protein-like 9 ( MGI:1923784)
Sequence:	sense strand is shown >T4957 GGCTCTCCTGCTGGCCGCTTGCGGAGGTTTGGATTCAGGATTCATCCCCAGTGTCCAAGACTTTGATAAG AAACTTACCGAGGCTGACGCGTACCTGCAGATCTTGATAGAACAATTAAAGCTTTTTGATGACAAGCTTC AAAATTGTAAAGATGATGAACAGAGAAAGAAAGTTGAAACTCTCAAAGACACAACAAATAGCATGGTAGA ATCAATTAAACACTGCATTGTGTTGCTACAGATTGCTAAAGACCAGAGTAATGCGGAGCAGCACGCAGAT GGAATTATAAGTACTATTAATCCTGTAGATGCAATATACCAGCCTAGTCCCTTGGAACCTGTGATCAGCA CAATGCCTTCCCAGACTGCCTTACCTCCAGAACCCGCTCAGTTGTGTAAGTCAGAGCAGCGTCCATCTTC CTTACCTGTTGGACCTGTGTTAGCTACCTTGGGACATCATCAGACTCCAACACCAAATAGTACAGGCAGT GGGAACTCACCACCTAGCAGCAGTCTGACTCCTCCCAGCCATGTCAACTTGTCTCCAAATACAGTCCCAG AGTTCTCTTACTCTAGCAGTGAAGATGAGTTCTATGATGCTGATGAATTCCATCAAAGTGGCTCGTCCCC AAAGCGCTTAATAGATTCTTCTGGATCTGCCTCAGTCTTGACACACAGCAGCTCCGGAAATAGCTTAAAA CGCCCAGATACCACAGAGTCTCTGAATTCCTCCATGTCCAATGGCACAAGCGATGCTGATCTTTTTGACT CACATGACGACAGAGATGATGATGGGGAGGCTGGGTCAGTGGAGGAGCACAAGAGCGTTATCATGCACCT CTTATCACAAGTCAGGCTGGGGATGGACCTCACAAAGGTAGTTCTTCCAACGTTTATTCTCGAGAGAAGA TCTCTGTTAGAAATGTATGCAGACTTTTTCGCACATCCAGACCTGTTCGTGAGCATTAGTGATCAGAAGG ATCCCAGGGATCGAATGGTTCAGGTTGTGAAATGGTACCTCTCGGCCTTCCATGCAGGAAGGAGAGGATC GGTGGCCAAAAAGCCGTACAATCCTATTTTGGGTGAGATCTTTCAGTGTCACTGGACGTTGCCGAATGAT ACTGAAGAGAACGCAGAGCTCGTTTCAGAAGGGCCGGTTCCCTGGGTTTCTAAGAACAGTGTAACATTTG TGGCTGAGCAAGTTTCCCACCATCCGCCCATTTCAGCCTTTTATGCTGAGTGTTTTAACAAGAAGATACA ATTCAATGCTCATATCTGGACTAAATCAAAATTCCTTGGGATGTCAATTGGGGTACACAACATAGGTCAG GGCTGTGTCTCGTGTCTGGAGTACGATGAGCACTACATCCTCACGTTCCCCAATGGCTATGGAAGGTCTA TCCTGACAGTGCCCTGGGTGGAATTGGGAGGAGAATGCAATATCAACTGCTCCAAAACGGGTTACAGCGC AAACATCGTCTTCCACACTAAGCCTTTCTATGGGGGCAAGAAGCACAGAATTACTGCAGAGATTTTTTCT CCGAATGACAAGAAATCCTTCTGCTCAATTGAAGGGGAATGGAATGGTATCATGTATGCAAAATACGCAA CAGGGGAAAACACTGTCTTTGTAGACACCAAGAAATTGCCTATAATCAAGAAAAAGGTGAGGAAGTTGGA AGATCAGAATGAGTATGAGTCCCGCAGCCTTTGGAAGGATGTCACTTTCAATTTAAAAATCAGAGACATT GATGCAGCAACGGAAGCAAAGCACAGACTTGAAGAAAGACAAAGAGCAGAAGCCCGAGAAAGGAAGGAGA AGGAAATTCAGTGGGAGACGAGGCTCTTTCACGAGGATGGCGAATGCTGGGTTTACGATGAGCCTTTACT GAAGCGTCTTGGTGCTGTGAAGCATTAGGCCGACAACCGAGTCCACACCTGGTGACCAGGGCAGTAGGCG TAATTAAGCAACAATCGATCTTCCTTCAGGAGAGCTTGTCACTTCCTTCTTAACGCAGTGGTTCCTATCT CAGGGATACTGGACTTGACGACACAGATGAACAATTAAAGTGGAAACCGCTTCCCTTTTCTCCTCTGTGG CAGTTACAATTTTGACTTCAGGCCTGAGAAAAACTTCAGGTTTCGAAACATGACATCTCTTCCTTTTCCA GATCCCATGCTTTGAAAAATATTTATAGACAGTTCCAGGTCTCAGCTTCCTGTCCTCTAGTTCTGCTGTT CGGGCATAAAATCTTTATCTCCAGTTCATATAATCTTGAGTTTTAGATATACACACATGCGTAACAGCTG ACAGTTTTTCACAAGTACACCCACCTGTAAATACTGTATCCTCAGTTTAGAAAATTAGTGCAATGTATGA AAATCAAGTGTTAGGAAATTTCATGGTTTCACCTATAACCTTTATTTTAGAATTGAACTATGATTAGATT GTATCTAAACCTGAAGTATAATTATATGCAGTGCTTCTTAAGGCTTCATAAAATAATTTTCCAACCTTTT TAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5134780 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5134780 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14178 same embryo
	EMAGE:14174 same embryo
	EMAGE:14176 same embryo
	EMAGE:14177 same embryo
	EurExpress:euxassay_003963 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source