EMAGE mouse gene expression spatial database

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EMAGE:14176

Hgfac hepatocyte growth factor activator ( MGI:1859281)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003956. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003956_01	euxassay_003956_02	euxassay_003956_03	euxassay_003956_04

euxassay_003956_05	euxassay_003956_06	euxassay_003956_07	euxassay_003956_08	euxassay_003956_09

euxassay_003956_10	euxassay_003956_11	euxassay_003956_12	euxassay_003956_13	euxassay_003956_14

euxassay_003956_15	euxassay_003956_16	euxassay_003956_17	euxassay_003956_18	euxassay_003956_19

euxassay_003956_20	euxassay_003956_21	euxassay_003956_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14176_wholemount_strong.wlz

14176_wholemount_moderate.wlz

14176_wholemount_weak.wlz

14176_wholemount_possible.wlz

14176_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14176_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver left lobe

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 weak expression: see section 01 02 03 04

liver right lobe

moderate

regional

moderate expression: see section 12 13 14 15 16 17 weak expression: see section 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4958
Entity Detected:	Hgfac, hepatocyte growth factor activator ( MGI:1859281)
Sequence:	sense strand is shown >T4958 GGAGGAGCCATGGGGCGACAGGCCTGGATCTCTAGCCTCTGTCCCTTACCCAGGCCATGTCCCTTCCTGC TGCTCCTGCTGCTGCTGGTGGTGCCTCGGGGGGCCCAGCCCCAGGCTGGCAGGAACCACACAGAGCCCCC AGGACCTAACGTTACAGCCACCCCCGTGACCCCCACGATCCCTGTGATCTCTGGGAATGTCAGCACCTCA ACAGAAAGTGCTCCAGCAGCAGAGACTGAGGGACCCCAAAGTGAGAGGTACCCTCCTCCCTCCAGCAGCA GCCCCCCTGGGGGCCAAGTGCTCACCGAGTCTGGGCAGCCGTGCAGGTTCCCTTTCCGCTACGGTGGCCG CATGCTGCACTCCTGTACCTCTGAGGGAAGTGCCTACAGGAAGTGGTGCGCTACAACACACAACTATGAC CGAGACCGGGCCTGGGGCTACTGTGCAGAGGTGACCCTGCCTGTGGAAGGTCCAGCTATCCTTGACCCTT GTGCCTCTGGGCCCTGCCTCAATGGGGGCACATGTTCCAGTACACATGACCATGGGTCCTACCACTGCTC TTGCCCCCTGGCCTTCACAGGCAAGGACTGTGGCACAGAGAAATGCTTTGATGAAACACGCTACGAGTAT TTTGAGGTGGGCGACCACTGGGCCCGTGTGAGCGAGGGACATGTGGAGCAATGTGGCTGTATGGAGGGCC AGGCCCGGTGTGAAGACACCCACCACACAGCTTGTCTGAGCAGCCCATGTCTGAACGGAGGCACCTGCCA CCTGATTGTGGGCACAGGGACCAGCGTCTGCACCTGCCCGTTGGGCTATGCTGGGCGGTTCTGTAACATT GTTCCCACAGAGCACTGCTTCCTGGGAAATGGTACAGAGTACCGAGGCGTGGCCAGCACCGCTGCCTCGG GCCTGAGCTGCCTGGCCTGGAATTCTGACCTGCTCTACCAGGAGCTGCACGTGGACTCAGTGGCTGCTGC TGTCCTGCTTGGCCTGGGCCCTCACGCTTACTGCCGGAACCCAGACAAGGATGAGAGGCCTTGGTGCTAT GTGGTGAAGGACAACGCACTGTCGTGGGAGTATTGCCGCCTGACAGCCTGTGAATCCCTGGCCAGAGTCC ACTCCCAAAGCCCGGAGATCCTAGCAGCCCTGCCCGAGTCAGCCCCGGCTGTGCGTCCTACCTGTGGCAA GAGGCACAAGAAGAGGACGTTCTTGAGACCACGCATCATCGGGGGCTCATCATCTTTGCCTGGCTCACAC CCCTGGCTGGCTGCCATCTACATTGGGAATAGCTTCTGTGCCGGGAGCCTTGTCCATACCTGCTGGGTAG TGTCTGCAGCCCACTGCTTCGCCAACAGCCCCCCCAGGGACAGCATCACAGTGGTACTGGGTCAGCACTT CTTCAACCGCACCACGGATGTGACACAGACATTTGGCATTGAGAAGTATGTGCCCTACACCCTGTACTCG GTGTTCAACCCCAACAACCATGACCTTGTCTTGATCCGGCTAAAGAAGAAGGGAGAGCGCTGTGCTGTCC GCTCCCAGTTTGTTCAACCCATCTGCCTGCCTGAGGCAGGCAGCTCCTTCCCTACTGGACACAAGTGTCA GATTGCAGGCTGGGGCCACATGGATGAAAATGTGAGCAGCTACTCCAACTCCCTGCTGGAGGCACTGGTC CCTCTTGTTGCTGACCACAAGTGTAGCAGCCCAGAGGTATATGGTGCTGACATCAGCCCTAACATGCTCT GTGCCGGCTACTTCGACTGCAAGTCCGATGCCTGCCAGGGGGACTCAGGTGGGCCCTTGGTCTGTGAGAA GAATGGTGTGGCTTACCTGTATGGCATCATCAGCTGGGGTGATGGCTGTGGGCGCCTCAACAAGCCAGGA GTCTACACCCGTGTGGCCAATTATGTGGACTGGATCAACGACCGTATTCGACCGCCCAAGCGACCCGTGG CTACGTCCTGAACCCCGGTCCCCAAGGAAGGGATGCTCATAGTACCCCTTCTACTACCAACAATAAAACT GTCTCCAAGGACCTGGAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5134851 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5134851 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14178 same embryo
	EMAGE:14174 same embryo
	EMAGE:14177 same embryo
	EMAGE:14175 same embryo
	EurExpress:euxassay_003956 same experiment
	MGI:4825368 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source