EMAGE mouse gene expression spatial database

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EMAGE:14180

Myot myotilin ( MGI:1889800)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003966. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003966_01	euxassay_003966_02	euxassay_003966_03	euxassay_003966_04

euxassay_003966_05	euxassay_003966_06	euxassay_003966_07	euxassay_003966_08	euxassay_003966_09

euxassay_003966_10	euxassay_003966_11	euxassay_003966_12	euxassay_003966_13	euxassay_003966_14

euxassay_003966_15	euxassay_003966_16	euxassay_003966_17	euxassay_003966_18	euxassay_003966_19

euxassay_003966_20	euxassay_003966_21	euxassay_003966_22	euxassay_003966_23	euxassay_003966_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14180_wholemount_strong.wlz

14180_wholemount_moderate.wlz

14180_wholemount_weak.wlz

14180_wholemount_possible.wlz

14180_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14180_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foot mesenchyme

moderate

regional

moderate expression: see section 07 18 19

knee mesenchyme

moderate

regional

moderate expression: see section 01

upper leg mesenchyme

moderate

regional

moderate expression: see section 04 05 06 07 08 09 16 17 18 19 20

head mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 19 20

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20

tongue

moderate

regional

moderate expression: see section 10

tongue mesenchyme

moderate

regional

moderate expression: see section 10 11 12 13 14 15

tail mesenchyme

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4149
Entity Detected:	Myot, myotilin ( MGI:1889800)
Sequence:	sense strand is shown >T4149 GGAAATGCTTCTCCCTTCTCTTCAGTAGTGGGTTGAGACGCTGGCCCCCTCTGAGCTAAGGAAACAATAT TCTTCAAGAGAAACCCACCAACATCAGGAGTACAGTGGTGGTCTAGAGATCTCAGCAAAGAGGGCCAGAC TAAAGTTGCTTCTGACTTGCTGCAACCTTCTGTGTAATTCCAGGCTTGCCAACCCCACGGTCAGGATTCT ATCCTTCTGTGAGCAAATCTGTCTTCCATATACCAGCTAAGCATGTTTAACTACGAACGTCCAAAACACT TCATCCAGCCGCAAAATCCGTGTGGCTCCAGACTGCAGCCTCCAGGACCGGAAGTCTCCGGCTTCCCGAG CCAGACCAAACAGTCTTCCATCGTCATCCAGCCTCGCCAGTGCACAGAGCAGAGATTTTCTGCCTCCTCC ACAGTGAGCTCTCACATCACCGTGTCTTCCTCTGCTTATCCTGCTCCCCAGCAGCTTGCTGGCCCCAACC CCGGCCAAAAGGTTACTGCTACCTATAACCAGTCCCCAGCCAGCTTCCTCAGTTCTATATTACCGTCTCA ACCCGATTACTGTAACAGTAAAATCCCATCCACTGTGGACTCCAACTATCAACAATCCTCAGTTAACCAA CCTGTAAATGCCATGTCATCCCAAGCTGCAAACGCTAGGCCCACTCCAAAGACTCCTGACCATGAGATCC AAGGATCGAAAGAAGCGTTGATTCAAGATTTGGAAAGAAAGCTGAAATGCAAAGACACCCTTCTTCATAA TGGAAATCAAAGACTGACCTACGAGGAGAAGATGGCTCGCAGGTTGTTAGGACCGCAGAATGCTGCTGCT GTGTTTCAGGCGCAAAACAGTGATGTCCAAGATTCACCACAGCATAACCCAGAGCAAGCACGGCTGCACG TTCCCACATCACAAGTAAGAAGTAGATCGTCCTCCAGAGCAGAGGCCAATGATCAGGATGCCATCCAGGA GAAGTTTTACCCACCACGCTTCATTCAAGTGCCAGAAAACATGTCGATCGAAGAGGGGAGGTTCTGCCGA ATGGACTTTAAAGTAAGTGGACTGCCAGCCCCTGATGTGTCATGGTATCTAAACGGGAGACCAGTGCAAT CAGATGAGTTGCACAAAATGATAGTGTCTGAGAAGGGTTTCCACTCCCTCATCTTCGAAGTGGTCAGAGC ATCAGATGCAGGGCCATATGCATGCGTGGCCAGGAACAGAGCTGGAGAAGCCACCTTCACAGTGCAGCTG GATGTTCTGGCAAAAGAGCATAAAAGAGCACCGATGTTTATCTTCAAACCACAGAGTAAGAAAGTTTTTG AGGGAGAGACCGTGAAGCTGGAGTGCCAGATCTCAGCTATCCCTCCACCAAAGCTGTTCTGGAAAAGAAA CAATGAAATGGTCCAGTTCAACACTGATCGGATAAGCTTATATCATGACAACGCCGGAAGAGTAACTTTA CTGATAAAAGATGTAAACAAGAAGGACGCTGGGTGGTATACAGTGTCAGCAGTCAATGAAGCTGGTGTGA CCACGTGTAACACACGATTAGATGTCACAGCCCGTCCAATCCAAACTCTCCCAGCTCCTAAGCAGCTGCG TGTTCGACCAACATTCAGCAAATATTTAGCGCTCAATGGGAGAGGTTTGGATGTGAAACAAGCTTTTAAC CCTGAAGGAGAATTTCAGCGCCTGGCAGCTCAATCCGGACTCTACGAAAGTGAAGAACTTTAATAACTTT GCCCACAGGGAAAACACAACTTCGCTACAACCAATATATCTGATAACATTGTGGGAAAAAAAATCCATGG CTATATTAACAAATTGTGACTTTACTTAGGTCGTTTAACTAACTCACATTTAGAATGTTATTTAGCCTCT GACTCTGGCACAGCCTGTATTCCTCTGACTTGTGAAGCCAACAGTAAACTAAGTATATGAGTTCGTATTG TAAGAGACTGTTTTAAGATGCAGTGTCTTAACACTGAAGTCCTTTAACAACTGTGAGTTATGAACCAGAA ACTTTTGAAACACCACTAATGCTTGCAATAAGTTTATGGGTATTGCTTAACAAACATTCATTTACCTGTT TTCTCTTATAGGAATACTAACATGATATAGATTATCTGAGTGTTCCACAACTTCCCATCAAAAGAGAATA AAAATTTGAATAACTAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4482382 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4482382 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14183 same embryo
	EMAGE:14182 same embryo
	EMAGE:14179 same embryo
	EMAGE:14184 same embryo
	EMAGE:14181 same embryo
	EurExpress:euxassay_003966 same experiment
	MGI:4826571 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source