EMAGE mouse gene expression spatial database

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EMAGE:14242

Gna13 guanine nucleotide binding protein, alpha 13 ( MGI:95768)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001786. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001786_01	euxassay_001786_02	euxassay_001786_03	euxassay_001786_04

euxassay_001786_05	euxassay_001786_06	euxassay_001786_07	euxassay_001786_08	euxassay_001786_09

euxassay_001786_10	euxassay_001786_11	euxassay_001786_12	euxassay_001786_13	euxassay_001786_14

euxassay_001786_15	euxassay_001786_16	euxassay_001786_17	euxassay_001786_18	euxassay_001786_19

euxassay_001786_20	euxassay_001786_21	euxassay_001786_22	euxassay_001786_23	euxassay_001786_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14242_wholemount_strong.wlz

14242_wholemount_moderate.wlz

14242_wholemount_weak.wlz

14242_wholemount_possible.wlz

14242_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14242_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4396
Entity Detected:	Gna13, guanine nucleotide binding protein, alpha 13 ( MGI:95768)
Sequence:	sense strand is shown >T4396 CGCAGGGGCCCGCCGAGGCGGCGGCGGCGGCAAGATGGCGGACTTCCTGCCGTCGCGCTCCGTGCTGTCC GTGTGCTTCCCGGGCTGCGTGCTGACGAACGGCGAGGCCGAGCAGCAGCGCAAGTCCAAGGAGATCGACA AATGCCTGTCGCGGGAGAAGACCTACGTGAAGCGGCTGGTGAAGATCCCGCTGCTGGGCGCGGGCGAGAG CGGCAAGTCCACCTTCCTGAAGCAGATGCGGATCATCCACGGCCAGGACTTCGACCAGCGCGCGCGCGAG GAGTTCCGCCCCACCATCTACAGCAACGTGATCAAAGGTATGAGGGTGCTGGTAGATGCCCGAGAGAAGC TTCATATTCCCTGGGGAGATAACAAAAACCAGCTCCATGGAGACAAGTTGATGGCATTTGATACCCGCGC CCCCATGGCTGCCCAGGGGATGGTGGAGACTCGAGTGTTCCTGCAGTATCTTCCTGCTATCAGAGCCTTA TGGGAGGACAGTGGTATACAGAATGCCTACGATCGGCGCCGGGAATTCCAGCTGGGTGAGTCTGTAAAGT ATTTCTTGGATAACTTGGATAAACTTGGAGTACCGGATTACATTCCATCACAGCAAGATATCCTGCTTGC CAGAAGGCCCACCAAAGGCATCCATGAGTACGACTTTGAAATTAAAAATGTTCCTTTCAAAATGGTTGAT GTAGGTGGCCAGAGATCAGAACGGAAACGCTGGTTTGAATGCTTTGACAGTGTGACGTCGATACTTTTCC TTGTCTCTTCAAGTGAATTTGACCAGGTGCTTATGGAGGACCGCCAGACCAATCGCCTTACAGAATCTCT GAACATTTTTGAAACAATTGTCAACAATCGGGTTTTCAGCAACGTCTCCATAATCCTCTTCTTAAACAAG ACAGACTTGCTCGAGGAGAAAGTGCAAGTTGTTAGCATCAAAGACTATTTCCTAGAATTTGAAGGGGACC CCCACTGCTTAAGAGACGTCCAAAAGTTTCTGGTGGAATGCTTCCGGGGGAAACGCCGGGACCAGCAGCA GAGGCCGTTGTACCACCACTTCACCACCGCGATCAACACAGAGAACATCCGCCTCGTGTTCCGGGACGTG AAGGACACGATCCTTCATGACAACCTGAAGCAGCTCATGCTGCAGTGATGACAAGACTGGCTGTTTTAAT ACCTTGTTGTGATTTTGATTGTTTTCTGTTTGTCTTGTTTTTCTCTTTAAATGGCAGTTTACAACAGCAG TTAGAAGAATCTCAGTTGTGTGTGTCTAACTTCTTGGAGAACTTAGTTCATCTGTGGCCTTTAGTTTGTG GCTGACAGCTGCTGAATTAACCCATTGCCAATATCTGCTAGAATTTGGGCTTTGATTGGTTTCACTGTGG CTTACCTTCAAGTCCAATTATAACTTCCTGGCAACCTTACACTAGGTGTTTTTCAGGCCTTGAATTCTCA TAACTGTCCTGCAGTTCTAAGAATAGGCAGTATCTTTAAGAACTTGTAGGGATGAGAGTATGAAAATTCA GCTCTAAAGAGAGAGTGCCTTACCCAGCAATGACATGCCTCGCTATGTGAGCCCTGGTCTGGAAGGGCTG TTAGAGAAAAGTTTTAGGGAGGAGTCTCGGCCCACAGAGGCTTGGCCCCCTACACACACAGCAGTAGTTT GTTTTTCTCAATCTCTTTACTTTCTGAAGGTAATGTCTTACTGCTTAAGTTTACAAGATGGATCTCAGCC TGATGCTGATGCGGTCACAAATGGTACATGTGCTATGTTGATCTGAGATGAGCAGTGACACGCAGTTTCC TTTGCCATACATGCTGTCACTAACTTAATGTGCCATGTTACAACATCTGGAGAGGGCAAGCACCCTGGTG ACGGACATCACCATAGCAGCCCCAGAGTGTGACTTTCTGGTCCTGTCTCCTTGGACTTTGTGCCTTGCTG GAGGGAACCACTGTTGGGTCTTCTTCCTTTTGCCCCACTGTCTCAACCTCAGCAGCTCTGAACTGTGGTG CTCTCGGGCTTCTCTTGTCCCCGAGAGCTTGGTGGGCAGCATTTCCCTGTGAACTGTTCAAAAAGGCATC CTTTCAGTACCCTGTCCCTGGTTAGTGTCTCTCTGTGACAGAGTTTGTATGTGTTGTGTGTGTCATCGTG TGTGAGACACAGCTTCATTGAGGTACATTCAAATGTCTTAAGGATGCATCTAAGGATAAGCAATTTTTTT TTTGGCCCATACTTAAGGAGGTTTGCCTTCACACACACACACACACACACACACACATACCATTGCTAAC TTAAACCATGCCCATGAGGTAGCTTGTGATAAAATACAGTGGCTGGTGTACAAAACCATTTGGCATGTTC CAACTGGCCTGGGAGTTTGTAGAACCTGAGCTTGTCCCTGCTGTCCCTGGAGAGCGCAAGAGGGCAGTGA AGCCTGTTTTATTGTGTGACTATTCTCAGGCAAGAAATTGCGCCCTGAAAGACTGGCTGACTATGAGGCC CAAAAGCAGTGGCTTTCTTCTTTTCTTGCTGGAGGGTGCCAGAGCTGGGCCCTAGTGTATGTATACTTGA GAGACCTGACTTGTCCTGGATTTGAGCTTTCACTGTCATTCTGGTTATAAAGTCCAGTGCTTTCTTGCCT TGGTAATATCATGAGCTTAAGACATGTATGTCAGTATCACAGTGCAGGTGTTGTTAGCATAATTCACCAT AATTCATGGTTCTACATCTGGTCCTTTTTCTGTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4918717 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4918717 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14245 same embryo
	EMAGE:14244 same embryo
	EMAGE:14247 same embryo
	EMAGE:14246 same embryo
	EMAGE:14243 same embryo
	EurExpress:euxassay_001786 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source