EMAGE mouse gene expression spatial database

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EMAGE:14246

Trpv6 transient receptor potential cation channel, subfamily V, member 6 ( MGI:1927259)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001795. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001795_01	euxassay_001795_02	euxassay_001795_03	euxassay_001795_04

euxassay_001795_05	euxassay_001795_06	euxassay_001795_07	euxassay_001795_08	euxassay_001795_09

euxassay_001795_10	euxassay_001795_11	euxassay_001795_12	euxassay_001795_13	euxassay_001795_14

euxassay_001795_15	euxassay_001795_16	euxassay_001795_17	euxassay_001795_18	euxassay_001795_19

euxassay_001795_20	euxassay_001795_21	euxassay_001795_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14246_wholemount_strong.wlz

14246_wholemount_moderate.wlz

14246_wholemount_weak.wlz

14246_wholemount_possible.wlz

14246_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14246_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 14 15 16 moderate expression: see section 03 06 17

stomach

strong

regional

strong expression: see section 04 05 06 moderate expression: see section 07

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4372
Entity Detected:	Trpv6, transient receptor potential cation channel, subfamily V, member 6 ( MGI:1927259)
Sequence:	sense strand is shown >T4372 CGGACGCGTGGGCGGCCAAGAGGAACAAAGCAGGAGCCCTCTTCAGGCTCCTAGGGGCAGCCACGGGAAG CCTCAGCAGCTCCACAGGTGAAGTAGGAGACAGAAGACAGGAGACGGGACCTCTACAGAGAGAGGACAGG CCGGCTCTTGGGGGTGCCAATGTGGCCCCAGGGTCGAGCCCAGTTGGGGTCTGGCATCAGCCTCAGCCCC CCAAGGAACCAGCCTTCCACCCCATGGGGTGGTCCCTGCCCAAGGAGAAGGGGTTAATACTCTGCCTATG GAACAAGTTCTGCAGATGGTTCCACAGACAAGAGTCCTGGGCTCAGAGCCGAGACGAGCAGAACCTGCTG CAGCAGAAGAGGATCTGGGAATCACCTCTTCTTCTAGCTGCCAAAGAAAACGATGTCCAGGCTCTGAGCA AGCTGCTCAAGTTTGAAGGATGTGAGGTGCACCAGAGAGGAGCCATGGGGGAAACTGCACTTCACATAGC AGCCCTCTATGATAACCTGGAGGCTGCAATGGTGCTAATGGAGGCTGCCCCAGAACTAGTCTTTGAGCCC ATGACTTCGGAGCTATATGAAGGTCAGACTGCACTACACATTGCAGTAATAAACCAGAATGTAAACTTGG TCCGTGCTCTGCTTGCCCGAGGGGCCAGTGTCTCTGCCAGAGCTACAGGCTCTGTCTTCCACTACAGGCC TCACAACCTCATTTACTATGGAGAGCACCCTTTGTCCTTTGCTGCCTGTGTGGGTAGTGAGGAGATTGTT AGACTGCTCATTGAGCATGGAGCTGACATCCGTGCCCAGGACTCTCTGGGAAATACAGTACTACACATAC TCATCCTGCAGCCCAACAAAACCTTTGCCTGCCAGATGTACAACTTGCTACTGTCCTATGATGGGGGAGA CCACCTGAAGTCCCTGGAACTTGTGCCAAATAACCAGGGACTCACCCCCTTCAAGTTGGCTGGGGTGGAA GGCAACATTGTGATGTTCCAACACCTGATGCAGAAGCGGAAACACATCCAGTGGACATATGGGCCATTGA CCTCCACACTGTATGACCTCACTGAGATTGACTCCTCAGGAGATGATCAATCCCTACTGGAACTTATTGT TACTACCAAGAAACGGGAGGCTCGCCAGATCCTGGACCAGACACCTGTAAAGGAACTGGTGAGCCTCAAG TGGAAGAGGTATGGGCGGCCCTACTTCTGCGTGCTGGGTGCCATCTATGTGCTGTACATCATCTGCTTTA CCATGTGCTGTGTCTACCGCCCGCTCAAGCCCAGGATCACTAACCGCACCAACCCCCGGGACAACACTCT GATGCAACAGAAGCTCCTTCAGGAGGCCTATGTGACCCCTAAGGATGACCTCCGGTTGGTGGGGGAACTC GTGAGCATCGTTGGGGCTGTGATCATCCTGCTGGTGGAGATTCCAGACATCTTCAGGTTGGGGGTCACTC GATTCTTTGGACAGACCATTCTCGGGGGGCCATTCCATGTCATCATTATCACTTATGCCTTCATGGTGCT GGTGACCATGGTGATGCGACTCACCAATGTAGATGGAGAAGTGGTGCCCATGTCCTTTGCTCTGGTGTTG GGCTGGTGCAATGTCATGTACTTTGCCAGAGGATTCCAGATGCTGGGTCCCTTCACCATCATGATTCAGA AGATGATTTTTGGTGACTTGATGCGATTCTGCTGGCTGATGGCTGTGGTAATTCTGGGATTTGCTTCAGC CTTCTATATCATTTTCCAGACAGAGGATCCCGATGAGCTGGGTCATTTCTATGACTACCCCATGGCACTG TTCAGCACCTTTGAACTCTTCCTCACCATCATCGATGGCCCTGCCAACTATGACGTGGATCTGCCCTTCA TGTACAGCGTTACCTACGCTGCCTTTGCCATCATCGCCACACTGCTCATGCTTAACCTCCTCATTGCCAT GATGGGTGACACTCACTGGAGAGTTGCCCATGAGCGAGATGAGCTCTGGAGAGCACAGGTTGTGGCCACC ACTGTGATGTTAGAGCGGAAGCTACCTCGTTGCCTGTGGCCTCGCTCTGGGATATGTGGGCGAGAGTATG GTCTTGGCGACCGCTGGTTCTTGAGGGTGGAAGACAGGCAAGATCTCAACAGACAACGCATCCGCCGCTA TGCACAGGCCTTCCAGCAACAAGATGGCCTCTACTCTGAGGACTTGGAAAAAGATTCAGGAGAAAAACTG GAGACAGCACGTCCCTTTGGTGCCTATCTGTCCTTTCCTACACCCTCAGTATCTCGAAGTACCTCCCGAA GCAGCACCAATTGGGAAAGGCTTCGACAAGGGGCCCTGAGGAAGGACCTGCGGGGGATAATCAACCGGGG CCTAGAAGATGGGGAGGGCTGGGAGTACCAGATCTGAATGTTGGCTCTCACCAAGCATCAGAACAGAATG AAAGAAAGACAGCTAAGGTCATCCTGCGAGTCCAAGGGAGAAGGAAGAGGAACATGCCAAGGAATGTACA CTGGGAATAAATGCTCCAGGGCTCCACAACTCCTTTATCTTGGAGCAAAAGGAAAAAAAAAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4911355 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4911355 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14245 same embryo
	EMAGE:14244 same embryo
	EMAGE:14242 same embryo
	EMAGE:14247 same embryo
	EMAGE:14243 same embryo
	EurExpress:euxassay_001795 same experiment
	MGI:4828944 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source