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EMAGE:14247

Hbb-b2 hemoglobin, beta adult minor chain ( MGI:96022)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14247 EMAGE:14247 EMAGE:14247 EMAGE:14247 EMAGE:14247
euxassay_001787_01 euxassay_001787_02 euxassay_001787_03 euxassay_001787_04 euxassay_001787_05
EMAGE:14247 EMAGE:14247 EMAGE:14247 EMAGE:14247 EMAGE:14247
euxassay_001787_06 euxassay_001787_07 euxassay_001787_08 euxassay_001787_09 euxassay_001787_10
EMAGE:14247 EMAGE:14247 EMAGE:14247 EMAGE:14247 EMAGE:14247
euxassay_001787_11 euxassay_001787_12 euxassay_001787_13 euxassay_001787_14 euxassay_001787_15
EMAGE:14247 EMAGE:14247 EMAGE:14247 EMAGE:14247 EMAGE:14247
euxassay_001787_16 euxassay_001787_17 euxassay_001787_18 euxassay_001787_19 euxassay_001787_20
EMAGE:14247 EMAGE:14247
euxassay_001787_21 euxassay_001787_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14247Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14247_wholemount_strong.wlz
14247_wholemount_moderate.wlz
14247_wholemount_weak.wlz
14247_wholemount_possible.wlz
14247_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14247_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
liver lobe
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4399
Entity Detected:Hbb-b2, hemoglobin, beta adult minor chain ( MGI:96022)
Sequence:sense strand is shown

>T4399
GTTGTGTTGACTTGCAACTTCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGTCTGCTGTC
TCTTGCCTGTGGGCAAAGGTGAACCCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTCT
ACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGGGTAATCC
CAAGGTGAAGGCCCATGGCAAAAAGGTGATAACTGCCTTTAACGAGGGCCTGAAAAACCTGGACAACCTC
AAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGC
TCCTGGGCAATGCGATCGTGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCCTGCTGCACAGGC
TGCCTTCCAGAAGGTGGTGGCTGGAGTGGCCACTGCCCTGGCTCACAAGTACCACTAAGCCCCTTTTCTG
CTATTGTCTATGCACAAAGGTTATATGTCCCCTAGAGAAAAACTGTCAAGTGTGGGGAAATGATGAAGAC
CTTTGGGCATCTAGCTTTTATCTAATAAATGATATTTACTGTCATCTCAAAAAAAAAAAAAAAAAAAAAA
AA
Notes:The probe template was PCR amplified from IMAGE:4920093 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4920093 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14245 same embryo
 EMAGE:14244 same embryo
 EMAGE:14242 same embryo
 EMAGE:14246 same embryo
 EMAGE:14243 same embryo
 EurExpress:euxassay_001787 same experiment
 MGI:4825330 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS