EMAGE mouse gene expression spatial database

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EMAGE:14256

Acp2 acid phosphatase 2, lysosomal ( MGI:87882)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003990. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003990_01	euxassay_003990_02	euxassay_003990_03	euxassay_003990_04

euxassay_003990_05	euxassay_003990_06	euxassay_003990_07	euxassay_003990_08	euxassay_003990_09

euxassay_003990_10	euxassay_003990_11	euxassay_003990_12	euxassay_003990_13	euxassay_003990_14

euxassay_003990_15	euxassay_003990_16	euxassay_003990_17	euxassay_003990_18	euxassay_003990_19

euxassay_003990_20	euxassay_003990_21	euxassay_003990_22	euxassay_003990_23	euxassay_003990_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14256_wholemount_strong.wlz

14256_wholemount_moderate.wlz

14256_wholemount_weak.wlz

14256_wholemount_possible.wlz

14256_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14256_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4971
Entity Detected:	Acp2, acid phosphatase 2, lysosomal ( MGI:87882)
Sequence:	sense strand is shown >T4971 CAGTGATGGCCGGCAGACAGACTGGTTGGAGCCAGGCGGCTCTTCTCCAGTTCCTTCTTGGCATGTGCCT AACGGTGATGCCACCCATACAGGCCCGGAGTCTACGCTTTGTTACCTTGCTGTATCGACATGGAGATCGG TCACCAGTGAAGACATATCCCAAGGACCCCTATCAGGAAGAGAAATGGCCCCAGGGATTTGGTCAGCTAA CCAAGGAAGGGATGCTACAGCACTGGGAGCTGGGCCAGGCCCTGCGGCAACGCTACCATGGCTTTCTGAA CACCTCTTACCACAGGCAAGAGGTTTATGTGCGAAGCACAGACTTCGATCGTACTCTCATGAGTGCTGAG GCCAACCTGGCTGGACTCTTCCCTCCCAATGAAGTTCAGCACTTCAACCCTAACATTTCATGGCAGCCTA TCCCTGTTCACACTGTGCCCATCACTGAAGACAGGTTGCTGAAGTTTCCTTTGGGTCCATGTCCCCGTTA TGAGCAGCTGCAGAATGAGACTCGGCAGACACCAGAGTATCAGAACAGAAGTATTCAGAATGCACAATTT CTGAACATGGTGGCCAACGAGACAGGGCTTACAAACGTGACCCTAGAGACCATCTGGAATGTGTATGACA CACTCTTTTGTGAGCAAACCCATGGGCTGCTTCTGCCGCCCTGGGCCTCACCCCAAACCGTGCAGCGTCT GAGCCAGCTAAAGGACTTCAGCTTCCTCTTCCTCTTCGGGATCCACGAGCAAGTACAGAAGGCCCGGCTT CAGGGGGGAGTTCTGCTGGCTCAGATATTGAAGAACCTGACCCTAATGGCAACTACCTCTCAATTCCCTA AGCTTCTGGTTTATTCCGCGCATGACACTACCCTGGTTGCTCTGCAAATGGCATTGAATGTCTACAATGG GAAGCAAGCTCCCTATGCTTCCTGCCACATATTTGAACTGTACCAGGAAGATAATGGGAATTTCTCAGTC GAGATGTACTTTCGGAATGACAGTAAGAAGGCACCCTGGCCTCTGATCCTGCCTGGCTGTCCTCACCGTT GCCCACTGCAGGATTTCCTTCGCCTCACAGAGCCTGTCATACCCAAGGACTGGCAGAAGGAGTGCCAGCT AGCAAATGATACTGCAGACACAGAGGTAATTGTGGCATTGGCTGTCTGTGGCTCCATCCTCTTCCTTCTC ATAGTGTTGCTCCTCACCATCCTCTTCCGGATGCAGGCCCAGCCTCCTGGCTACCACCATGTTGCAGACA GGGAAGACCATGCTTGACAACCACTCAGTCCCCTTCCCTCTGCCTCCTAGGGGAGGTGGGCTGAGTCCTC GCTCCTGACCATTGCTGATCCCAGCCCAGGGACAGGGTTAGCCTTCCTGATTACCTACGCCCAAATGAAT GAGTGAGGCTCGGCTGGGTGTGCGGCACCTGGGGCTCACCATCTTCATGCCTGATGTTTACCGTGTACTG TGTTGGACACTGGCTTTCTCTAAATGGGATTTGCCTCTTCTATATTCCCTAAGGACTTGAGATGCAGACA AGCATTCAGATTTCACTCAAGACCTGGGACTAAAAAACAAAACAACAAAACCCCCATGAAAACCTGAAGA AGCTGGTACTTGATCTGCCTTGCTCTTCCACCGAGCCTTGCTCTTTATCTTCTAGCCCATATCTTTGGCA AATGGCTTGGAGGACATTGTCCCTTGGTACTTTTTTAGAGGCAAAAACTGCAAATCACTAGGAGTATAAA ATATGGCTATCAAGAACTCGGGTCATCCAACAGCAAAGTCATCCTTTGGCTGAGGCTACCGTCAGTGTCA ACCACAGGCAGGTCTGAAGGATACTTGGGCTGTACCTGGATGACAGTGGGAGGACACAGGTAGGAGTGAA ACACATGGAATCATGAGGTGACTCTCCTCAGCTGGGCTGGACTCTTCAGGGACCGTACACCTTGACCGTG GCCCCAAATAGGGCTGAAGTAGCTCCGAGGAAATGAAGGTTTTGACTGTGAGTGCTGGTTCTGTGCCTTT TTTCCCTTCCCCATTTCTGACATCCAAGAAGCACTCTTACCAGGTCAGTACTAGATCTACTCAGAAACCC TGTTGGGCTTTTGGGTTTTTAGTTAGATACAAAGGATATGCTTTTATTGGCCATGCTAAAATTGAACATA AAAGTTTTAAAATTCTCTGGTTATTTTGCTCCTATACATCAGAAAAAAGAAAAGTACCTTGGCTCTAGCA TAAACTTGGCCTCCAGGCTAGATATAGCTGGGACCCTGAGCGTAGCTGGTTTACTCTTTAATTTTCTGGA GCAAAATCTAGAGCAGTAACATGTCAGCATCCTAGTCCCACAGTATCCCTTGTCACAAGTGTCCCTGCTC CAACCCTAAGAATTGGGTACTTTTGGCCTATTAACAAACAGCTGCCACTCCTCACAGAATGTGTTCCCAT CTGTGGGGTCCAGTATTGCTGTCCCACACCATCTTCATAGTCTTTCATGATCTTTCTGTGACCCATCTTC CTCTTGGCTCCAAATGAGAATATGATAACCTAGAAGAAACAAGAAGTCTGAATGACACCTTCTGTTGCTG GTCTGTCTCAGAGGTTGGGGCTGTCTTGGGGAACTAACAGACTGACATTGCCATGACTAAAATAAAACCA GATGGGTGACCTAGGCCAGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5136781 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5136781 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14259 same embryo
	EMAGE:14260 same embryo
	EMAGE:14258 same embryo
	EMAGE:14257 same embryo
	EMAGE:14261 same embryo
	EMAGE:14262 same embryo
	EurExpress:euxassay_003990 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source